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Biomedical subjects

M S Nelson

Publications and source records attributed to M S Nelson.

48 records · Page 3Linked to original sources

Pregnancy, diabetes, and Jewish dietary law: the challenge for the pregnant diabetic woman who keeps kosher.

One system of management for the pregnant woman with Type I diabetes is to instruct her in the strategies of achieving and maintaining normoglycemia with the use of self blood glucose monitoring, split doses of insulin, and titration of insulin to cover the meal plan. Normoglycemia is achieved only when the meal plan is adhered to in a rigid fashion to facilitate the insulin dose surrounding the meal. A 30 kcal/kg diet is divided into three meals and three snacks, of which 40% is composed of carbohydrate. These frequent small feedings must be perfectly timed to meet the insulin injected. Such systems require 35 hours of intensified training to become skilled in the techniques of diabetes self-care, but still the regimen does not provide much flexibility. When a pregnant woman also wishes to honor her religious beliefs by keeping kosher and so following another set of rigid laws surrounding the meal plan, adherence to both sets of rules becomes a challenge. Specifically, attention to separating milk and meat and eating only kosher foods must be part of the program. This article describes not only the basic diabetes self-care method but also how to adapt the method to the laws of keeping kosher while sustaining normoglycemia.

Blood Glucose↗

Acute urinary retention secondary to an incarcerated gravid uterus.

Acute urinary retention has many etiologies. In the pregnant patient, especially at the end of the first trimester, an incarcerated gravid uterus is a rare but possible cause. The patient discussed is a 30-year-old, gravida 4, para 2012, woman who was 14 weeks pregnant and who presented complaining of lower abdominal pain, urinary frequency, urgency, and nocturia. Based on the patient's symptoms, a diagnosis of a urinary tract infection was made, and antibiotic therapy was begun. Her symptoms failed to resolve, however, and she was referred for further evaluation, which eventually revealed an incarcerated gravid uterus. The patient was referred to an obstetrician who re-positioned her uterus without difficulty, and she subsequently had an uneventful pregnancy.

Adult↗

Lead toxicity masquerading as sickle cell crisis.

We recently saw a 12-year-old black boy with known sickle cell disease who had been seen many times for abdominal pain thought to be secondary to a vasoocclusive crisis. The patient eventually was admitted, after a seizure and the onset of obtundation. The etiology of his acute encephalopathy remained unclear until bone films of his knees fortuitously revealed "lead lines." The patient was treated and did well subsequently. This case emphasizes the importance of considering other diagnoses when a sickle cell patient presents with a crisis.

Anemia, Sickle Cell↗

Cutaneous B cell lymphoma: diagnostic use of monoclonal antibodies.

A patient presented with a solitary plaque initially interpreted on light microscopy as benign lymphoplasia. Monoclonal antibody studies were used to define the B cell origin of the infiltrate and to characterize its probable malignant potential. Monoclonal antibody typing is a useful technique to evaluate lymphocytic infiltrates that may be difficult to categorize on the basis of routine light microscopy.

Antibodies, Monoclonal↗

Three new phenotypes of human red cell acid phosphatase: ACP1FA, ACP1GA, and ACP1GB.

Three new phenotypes of human erythrocyte acid phosphatase (ACP1) have been detected and found to be unique by direct comparison with previously identified ACP1 variants. One of these new electrophoretic variants, labeled as ACP1FA, has been detected in the Hispanic population of California. The electrophoretic variants identified as ACP1GA and ACP1GB have been detected in a black family in North Carolina. A family study has shown that ACP1G is transmitted as an allele of ACP1.

Acid Phosphatase↗

A computer-assisted population frequency study of 14 polymorphic blood grouping systems in North Carolina.

Results of a population frequency study conducted on 14 polymorphic blood grouping systems in the North Carolina white and black populations are reported. A microcomputer program has been developed to facilitate the handling and storage of the large volume of data generated during the two years it took to complete this study. This computer program was also designed to calculate the combined phenotypic frequency for a given set of blood types.

Blood Group Antigens↗

A feasibility study of human leukocyte antigen (HLA) typing for dried bloodstains.

This paper constitutes a feasibility report on the use of the human leukocyte antigen (HLA) system for the typing of dried bloodstains. Antigens tested include the HLA-A2, A3, A10, B7, B8, and B14 alleles. An aging study conducted on A3 positive bloodstains showed that HLA-A3 could be reliably detected on bloodstains stored up to 30 days at 22 degrees C. Unlike most earlier reports on HLA typing of bloodstains, no cross-reactivity problems were detected with the antisera used in this study. In addition to the successful typing of bloodstains, we were also able to type fresh, neat seminal and saliva stains in the A2 and A10 antigenic systems.

Blood Grouping and Crossmatching↗

Biochemical and genetic characterization of the Lowell variant. A new phenotype of 6-phosphogluconate dehydrogenase.

A new electrophoretic variant of 6-phosphogluconate dehydrogenase (6PGD) has been detected in the Caucasian population in North Carolina, and family studies have shown this variant to be transmissible. This variant has been given the trivial name "Lowell" and the allele controlling its synthesis in conjunction with 6PGDA has been named 6PGDL. The banding pattern produced by the Lowell variant after electrophoresis is not affected by either NADP or 2-mercaptoethanol. Inhibition studies with urea and iodoacetate have shown that this variant is inhibited to approximately the same degree as the Richmond and Friendship variants. Thermostability studies have shown that the isozyme bands encoded by 6PGDA, 6PGDElcho, 6PGDC, and 6PGDL have a relative thermal stability in the order 6PGDA greater than 6PGDElcho greater than 6PGDC greater than 6PGDL.

Electrophoresis, Starch Gel↗

Detection of the rare PGM1(3) allele. Further biochemical and genetic characterization of the PGM1(3) isozymes.

A family possessing the rare PGM1(3) allele has been found in North Carolina, and criteria for the electrophoretic separation and accurate typing of the PGM1(3) isozymes are outlined. The PGM1(3) isozymes detected proved to be useful in helping to determine parentage in an incest investigation. The pattern of segregation of the PGM1(3) allele in four generations of this family and thermostability studies on the PGM1(3) isozymes are presented.

Alleles↗

Developmental expression and biochemical characterization Nassarius obsoleta.

Electrophoretic analysis of catalase isozyme patterns of Nassarius obsoleta indicates that these isozymes are products of two separate loci. Five aminopeptidase isozymes have also been detected in N. obsoleta and the data suggest that at least two loci encode these aminopetidase isozymes. Experiments designed to test for the interconvertibility of the isozymes indicated that the two catalase isozymes and that two of the five aminopeptidase isozymes tested were not conformational artifacts but distinct genetic products. No change in catalase isozyme expression, but considerable quantitative variation in catalase specific activity was noted during early developmental stages of N. obsoleta. Both qualitative and quantitative changes were noted in aminopeptidase expression during early developmental stages. This paper also describes several physicochemical parameters for each of the two enzymes under study.

Age Factors↗