Biomedical subjects

M S Mostafa

Publications and source records attributed to M S Mostafa.

At least 19 recordsLinked to original sources

PubMed2005

Amelioration of lead toxicity on rat liver with Vitamin C and silymarin supplements.

The aim of the present study was to investigate the impact of the combined administration of Vitamin C and silymarin on lead toxicity. Male albino rats were subdivided into three groups: the first was a control group, the second received lead acetate in diet as 500 mg/kg diet daily, the third received the same lead acetate dose and supplemented with Vitamin C (1 mg/100g body weight) and silymarin (1 mg/100g body weight) by gastric tube three times per week. Blood samples were taken after 2, 4 and 6 weeks of treatment. Significant lead-induced elevations in serum ALT, AST, GGT and ALP activities were observed after different periods of treatment. However, serum LDLc was decreased. The intensities of RNA and apoptotic fragments of DNA were measured as optical density by Gel-pro program. Lead acetate decreased the intensity of DNA at 6 weeks and induced apoptotic DNA fragments reversibly with time. After 2 weeks of lead administration dilation and congestion of terminal hepatic veins and portal vein branches were observed. Lead also induced hepatocyte proliferation without any localized distribution among zones 1-3. Portal inflammatory infiltrate with disruption of the limiting plates (interface hepatitis), steatosis, apoptosis and mild fibrosis were detected especially by sixth week of lead administration. Combined treatment of lead-exposed animals with Vitamin C and silymarin showed marked improvement of the biochemical, molecular and histopathological findings. These experimental results strongly indicate the protective effect of Vitamin C and silymarin against toxic effects of lead on liver tissue.

Administration, Oral↗

PubMed2004

Host-parasite relationships between Schistosoma mansoni and Echinostoma liei and their intermediate host Biomphalaria alexandrina using RAPD-PCR analysis.

Biomphalaria alexandrina are known to be intermediate hosts for both Schistosoma mansoni and Echinostoma liei. RAPD-PCR assay offers a new approach to host-parasite relationships. This was performed by investigating the genetic variation and compatibility among S. mansoni, E. liei and their intermediate host B. alexandrina with special emphasis on variations occurring in snails infected with S. mansoni and/or E. liei. Six primers were screened for DNA analysis and gave total patterns from 28 to 37 reproducible bands for each species. All specimens analyzed by the RAPD-PCR gave interpretable electrophoretic banding patterns that were polymorphic and compatible in the amplified products of these primers within each species.

Animals↗

PubMed2000

Cryptosporidiosis in Benha, study of some recent modalities in diagnosis and treatment.

A total prevalence of cryptosporidiosis was carried out on 1087 diarrheal patients of all age groups attending the outpatient clinics using modified Ziehl-Neelsen stain (MZN) and Meriflour direct immunofluorescent technique (IFA). IFA was more sensitive, specific and gave positive results 19.5% than MZN stain which gave a positivity 13.5% Cases were divided into 3 groups, each group was treated by one of nitazoxanid (NTZ), or co-trimoxazole or placebo. The most effective drug was NTZ which cured 39 patients (79.6%) out of 49 patients followed by co-trimoxazole that gave cure of 20 (41.6%) out of 48 patients. Placebo cured 20 (40%) out of 50 patients.

Adolescent↗

PubMed1990

Epidemiology of breast cancer, descriptive and analytical review.

The descriptive portion of this review explores briefly the historical literature for breast cancer. Also, it discusses occurrence, morbidity, mortality, personal risk factors, geographical distribution, effects of time and the latent period of breast cancer. The analytical portion of this review describes the role of genetics, viral and bacterial agents, glandular secretion and diet on the pathogenesis of breast cancer. In conclusion a variety of interrelated genetic, environmental and endocrine factors appear to be associated with increased risk of breast cancer, but no single factor or combination of variables presently known is sufficient to explain the etiology of this malignancy.

Breast Neoplasms↗

PubMed1987

Prevalence of trachoma in school children and ophthalmological outpatients in rural Egypt.

The prevalence of trachoma in school children and ophthalmological patients in rural villages of the Qalyub Governorate of Egypt was determined by clinical and laboratory diagnostic procedures and reported as mild, moderate, or severe according to the WHO classification scheme. Of 777 primary school students examined in 3 villages, 204 (26%) had clinically active trachoma. The overall prevalence of the disease in this population ranged from 16% to 35%. The prevalence of infection was higher in younger groups and decreased throughout primary school. Of 312 patients with ocular complaints examined at the village outpatient clinics, 100 (32%) had trachoma infections. Monoclonal FA staining showed higher sensitivity in detecting positive cases of trachoma than did Giemsa staining. This study has shown that trachoma is still prevalent in rural Egypt and that the monoclonal FA staining is a relatively sensitive and practical test for the laboratory diagnosis of trachoma in a field study, where reasonable facilities for culture diagnosis are not available.

Adolescent↗

PubMed1986