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Biomedical subjects

M S Collins

Publications and source records attributed to M S Collins.

At least 73 records · Page 4Linked to original sources
PubMed1984

Preparation and characterization of a nontoxic polysaccharide-protein conjugate that induces active immunity and passively protective antibody against Pseudomonas aeruginosa immunotype 1 in mice.

Acid treatment of Pseudomonas aeruginosa immunotype 1 lipopolysaccharide generated a low-molecular-weight polysaccharide fraction that was detectable in agar gel immunodiffusion but did not induce antibodies or resistance to infection in mice. The polysaccharide was treated with periodate to generate additional aldehyde groups. Oxidized polysaccharide was covalently coupled by reductive amination to 1,4-diaminobutyl-derivatized bovine serum albumin. Physical properties of the conjugate were characterized by gel filtration and high-pressure liquid chromatography. The gelation activity of the conjugate in the Limulus amoebocyte lysate assay was 4,000-fold less than native lipopolysaccharide by weight. Mice immunized with the conjugate resisted challenge with P. aeruginosa immunotype 1 that killed 90% of mice immunized with saline. Immunization with the conjugate vaccine induced humoral immunoglobulin G that passively protected normal and burned mice. These results indicate that conjugation of nonimmunogenic polysaccharide antigen of P. aeruginosa restores immunogenicity similar to that of native lipopolysaccharide without restoring endotoxicity inherent in lipopolysaccharide.

Animals↗
PubMed1984

Tumor models to investigate the therapeutic efficiency of immunomodulators.

The development of successful approaches to immunotherapy is dependent on the proper consideration of the pathobiology of metastasis and a better understanding of the principles of biological response modification. Immunomodulation as a therapeutic modality has not proven notably effective in animals with preexistent palpable disease, an observation in agreement with the results from clinical protocols. Therefore, rather than developing experimental immunotherapy models of immunoprophylaxis or models with minimal tumor burden, we believe it more appropriate to develop models based on preexistent metastatic disease in a tumor-conditioned host. The most rigorous test of a biological response modifier's efficiency prior to clinical trials will be provided from immunotherapeutic trials of palpable autochthonous tumors.

Acetylmuramyl-Alanyl-Isoglutamine↗
PubMed1983

Characterization of viruses which represent further distinct serotypes (PMV-8 and PMV-9) of avian paramyxoviruses.

Representatives of paramyxoviruses isolated from waterfowl in the U.S.A., goose/Delaware/1053/76, and Japan, pintail/Wakuya/20/78, were shown to be serologically closely related but distinct from other avian paramyxoviruses. Another isolate, from domestic ducks in the U.S.A., was shown to be representative of a further distinct serotype of avian paramyxoviruses. We propose that, in the current system of nomenclature these serotypes be designated PMV-8, for which the prototype strain would be PMV-8/goose/Delaware/1053/76 and PMV-9, for which the prototype strain would be PMV-9/domestic duck/New York/22/78.

Animals↗
PubMed1983

Anti-Pseudomonas aeruginosa activity of an intravenous human IgG preparation in burned mice.

A commercially available human IgG chemically modified for intravenous infusion (IGIV) was tested in burned mice for activity against eight strains of Pseudomonas aeruginosa. An 8.7 to 9.6% full-thickness burn was made with a gas flame on the shaved backs of anesthetized mice. A suspension of P. aeruginosa in 0.5 ml saline was then injected subcutaneously in the burn site. Inocula included seven American Type Culture Collection reference strains representing the seven Fisher-Devlin-Gnabasik immunotypes plus an additional strain of immunotype 1. IGIV immunotherapy did not significantly reduce mortality in burned mice challenged with immunotypes 5 and 6 but was highly protective against immunotypes 1-4 and 7. Groups of mice challenged with these five types and treated with approximately 100 or 400 mg IGIV/kg body weight had cumulative mortality rates at 15 days ranging from 0 to 30%, versus an overall mean 84.3% mortality in human serum-albumin treated controls. IGIV was protective if given up to 12 hours after challenge. These data indicate that IGIV has significant in vivo activity against P. aeruginosa and suggest that IGIV immunotherapy may be of value in the treatment of major thermal trauma in man.

Animals↗
PubMed1982

Identification of paramyxoviruses isolated from birds dying in quarantine in Great Britain during 1980 to 1981.

During 1980 to 1981 a total of 61 paramyxoviruses were isolated from imported captive birds dying during the quarantine period required under the Importation of Captive Birds Order 1976. These viruses were identified as avian paramyxovirus serotype PMV-1, 13 isolates (eight Newcastle disease viruses of high virulence, five of low virulence); PMV-2, 38 isolates; PMV-3, nine isolates and one isolate provisionally typed as PMV-7.

Animals↗
PubMed1981

The structural polypeptides of avian paramyxoviruses.

The structural polypeptides of twenty-three avian paramyxoviruses from five serotypes were examined by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulphate under reducing and non-reducing conditions. All virus polypeptide profiles consisted of 7--10 polypeptides of which two were glycosylated. Some variation was seen in the profiles of viruses from the same serotype, but groups formed on the basis of their serological relationships in haemagglutination inhibition tests were identical to those formed on the basis of similarities in their polypeptide profiles.

Antigens, Viral↗
PubMed1981

Paragonimus westermani: a cause of cavitary lung disease in an Indochinese refugee.

A Laotian immigrant with persistent cough and hemoptysis was found to have multiple small cavities on chest roentgenogram. Ova of the lung fluke Paragonimus westermani were found in the sputum, and the patient responded to bithionol therapy. Pulmonary paragonimiasis should be considered in the differential diagnosis of cavitary lung disease in Indochinese refugees entering the United States.

Adult↗
PubMed1980

Avian infectious bronchitis virus structural polypeptides: effect of different conditions of disruption and comparison of different strains and isolates.

Variations in the conditions used for disruption of purified virus involving differences in heat treatment and reducing agent concentration produced little affect on the polypeptide profiles of the Massachusetts 41 (M 41) strain of avian infectious bronchitis virus obtained by polyacrylamide gel electrophoresis. Comparisons of the structural polypeptides of 12 IBV isolates, consiting of M 41, six serologically related viruses and representatives of five other serotypes, showed that the viruses could be placed in three groups on the molecular weights of the major glycopolypeptides. These were 31,000 and 86,000 for M 41, the six related viruses and the serologically distinct SE 17; 27,000 and 89,000 for Iowa 97 and Holte and 27,000 and 91,000 for Connecticut and T strain.

Centrifugation, Density Gradient↗
PubMed1980

The polypeptide composition of isolated surface projections of avian infectious bronchitis virus.

Disruption of avian infectious bronchitis virus (IBV) particles with 4% Triton X-100 and 1.0 M-KCl and centrifugation through a sucrose gradient containing 0.1% Triton X-100 and 1.0 M-KCl enabled separation of the petal-shaped surface projections. By negative-contrast electron microscopy the separated projections appeared mainly as rosettes containing 3 to 12 projections radiating from a central core, although single projections and rosettes containing up to 16 projections were seen. SDS-PAGE of these preparations revealed two polypeptides of 86,000 and 66,000 mol. wt. The larger polypeptide was glycosylated.

Coronaviridae↗
PubMed1980

Isolation and properties of viruses from poultry in Hong Kong which represent a new (sixth) distinct group of avian paramyxoviruses.

Eight viruses isolated in Hong Kong were shown to be serologically related. One was obtained from the tracheal swab of a chicken and four were from cloacal swabs of ducks sampled at a poultry dressing plant. Three isolations were made from samples taken at a duck farm: two from pond water and one from faeces. Representatives of these isolates were shown to be paramyxoviruses but were serologically distinct from other avian and mammalian paramyxoviruses by haemagglutination inhibition and neuraminidase inhibition tests. Slight variations were seen in the properties of three isolates examined in detail. All three were apathogenic for chickens. The structural polypeptides of one isolate, PMV-6/duck/Hong Kong/199/77, were examined by SDS-polyacrylamide gel electrophoresis. Seven polypeptides were detected, with mol. wt. 180000, 76000, 60000, 55000, 51000, 48000 and 40000. The isolates represent a sixth serologically distinct avian paramyxovirus group.

Animals↗
PubMed1979

Properties of a newly isolated, serologically distinct avian paramyxovirus.

The morphological, bio-physical and growth properties of the isolate duck/Hong Kong/D3/75 (D3/75) were consistent with this virus being a member of the paramyxovirus group. Using haemagglutination inhibition and neuraminidase inhibition tests no serological relationships between D3/75 and other paramyxoviruses could be demonstrated. The structural polypeptides of D3/75 were also typical of paramyxoviruses, consisting of 6--7 polypeptides ranging in molecular weight from 46,000--200,000 under reduced conditions. Two polypeptides were glycosylated.

Animals↗