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Biomedical subjects

M Rupp

Publications and source records attributed to M Rupp.

At least 19 recordsLinked to original sources
PubMed1989

Quinoprotein ethanol dehydrogenase from Pseudomonas.

Dye-linked ethanol dehydrogenases from Pseudomonas aeruginosa ATCC 17,933 and P. putida ATCC 17,421 were purified to homogeneity and crystallized. The amino acid composition of the two enzymes is very similar and the number of the aromatic amino acid residues found per subunit are almost identical. With respect to their catalytic and molecular properties both ethanol dehydrogenases are similar to the quinoprotein methanol dehydrogenases known from methylotrophic bacteria. They show a high pH-optimum, need ammonia or an amine as activator and are dimers of identical subunits of a molecular mass of 60,000. The dimer is the catalytically active form. Each subunit carries one prosthetic group pyrroloquinoline quinone, which can be titrated by the suicide substrate cyclopropanone ethylhemiketal. In contrast to the general methanol dehydrogenases the two ethanol dehydrogenases have a low affinity for methanol and in addition to primary alcohols they also oxidize secondary alcohols. With secondary alcohols preferentially one of the two enantiomers is oxidized. The catalytic and spectral properties of the two enzymes are very similar to the quinoprotein ethanol dehydrogenase isolated from P. aeruginosa LMD 80.53 (Groen et al., 1984. Biochem. J. 223: 921-924). However this enzyme is reported to be a monomer of molecular mass 100,000.

Alcohol Oxidoreductases↗
PubMed1988

[Drug-induced alveolitis caused by salazosulfapyridine].

At onset of chronic rheumatoid arthritis a 36-year-old woman was started on a course of sulphasalazine. During the first four weeks the treatment she developed severe dyspnoea, mild fever, dry cough with chest pain, marked hypoxaemia and severely abnormal restrictive lung functions. Chest x-ray demonstrated diffuse alveolar-interstitial infiltrates. After discontinuing the drug and short-term administration of corticosteroids, blood gases and the chest x-ray reverted to normal within four weeks, but the abnormal lung functions persisted. The course of the illness and published reports on the side effects of sulphasalazine point to the need of carefully watching out for possible side effects during the first three months of treatment with this drug.

Adult↗
PubMed1988

Purification, crystallisation and characterization of quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa.

Pseudomonas aeruginosa ATCC 17933 when grown on ethanol produces high levels of a quinoprotein ethanol dehydrogenase, which amounts to 7% of the soluble protein. The enzyme has been purified to homogeneity and it crystallizes readily in the presence of polyethylene glycol 1550 or 6000. The ethanol dehydrogenase (Km(ethanol) = 14 microM) resembles the dye-dependent quinoprotein methanol dehydrogenases of methylotrophic bacteria, but has a low affinity for methanol (Km (methanol) = 94mM). In addition the enzyme oxidizes secondary alcohols. With its catalytic properties the ethanol dehydrogenase is similar to the enzyme isolated from P. aeruginosa LMD 80.53 (Groen, B., Frank, J. Jzn. & Duine, J.A. (1984) Biochem. J. 223, 921-924). In contrast to this enzyme from P. aeruginosa LMD 80.53, which is a monomer, the ethanol dehydrogenase isolated from P. aeruginosa ATCC 17933 is a dimer of identical subunits of relative molecular mass 60,000. The N-terminal amino acid is lysine. Inactivation with cyclopropanone ethylhemiketal reveals one molecule of pyrroloquinoline quinone per subunit. As shown by active enzyme sedimentation, the dimer is the enzymatically active form.

Alcohol Oxidoreductases↗
PubMed1988

[Infusions of amino acid solutions in the therapy concept of trauma patients. Comparative studies in the early phase of polytrauma].

The early phase of injury is characterized, among other things, by enhanced proteolysis and changed protein synthesis. Posttraumatic catabolism with its negative nitrogen balance exercises a direct influence on the healing process. Our study aimed at finding out whether early application of complete parenteral feeding would improve protein metabolism. Two groups of patients were compared with one another: One group of test persons (n = 28) whom a complete solution (2.5 carbohydrate and 10% AA solution) was given directly after the injury, whereas a control group (n = 71) received parenteral feeding only from the first or second posttraumatic day. The serum levels of proteins and nitrogen loss in the urine served as parameters for the effectivity of infusion therapy, besides the determination of the serum glucose level (which was clearly lower than in the control group). A significant improvement was established in total protein and albumin (p less than 0.01) compared with the control group. Proteolytic activity, measured via the nonspecific plasma proteinase and alpha 1-antitrypsin activity, increases in the same measure as the protein level drops. Urea nitrogen also drops significantly, thus expressing better utilisability (p less than 0.005). Taking as basis a hypocaloric parenteral diet (5 mg./kg. body weight/min.) we can assume that the utilization of the amino acid intake will be optimal. It became evident that the selected ration of nitrogen-free to nitrogen-containing calory carriers was insufficient on the first posttraumatic day. Amino acids are required at an early stage after polytrauma to ensure rapid and complication-free regeneration of the patient.

Acute-Phase Proteins↗
PubMed1978

[Repetitive work and psychosomatic complaints].

200 workers of the Swiss watch industry were examined in an interdisciplinary study on the effect of repetitive work on the wellbeing of the worker. Women doing repetitive work with little autonomy complained more often about psychosomatic problems than the male workers doing non-repetitive work. This difference is interpreted as a difference of sexe rather than one of the work situation. However, there is a significant difference in the complaint about nervosity between women being paid monthly and women who were paid by piece or by hour with a premium.

Boredom↗
PubMed1978

[Elimination of meproscillarin by hemoperfusion (author's transl)].

Starting from toxic concentrations of the new scilla glycoside 14-hydroxy-3beta-[(4-O-methyl-alpha-L-rhamnopyranosyl)-oxy]-14beta-bufa-4,20,22-trienolide (meproscillarin, Clift) 5 in vitro hemoperfusions with the hemoperfusion system Haemocol are described. The test showed that meproscillarin may be rapidly eliminated from the blood by adsorption to activated charcoal (hemoperfusion). The text provides basic information for possible intoxications.

Adsorption↗
PubMed1977

[Short- and long-term prognosis of cardiac arrest in acute myocardial infarction (author's transl)].

Of 80 patients with acute myocardial infarction who had a cardiac arrest without shock 42 (52.5%) were resuscitated in a cardiological intensive care unit. Twenty-six were finally discharged from hospital. After an average of four years, 21 patients were still alive. Prognosis of primary ventricular fibrillation, the most frequent cause of circulatory arrest (51) was more favourable than that of primary asystole (23 patients). The younger the patient the better the prognosis: the average age of the 21 who survived for several years was 12 years less than those who had died. Follow-up examination indicated that 13 had signs of heart failure. Ventricular extrasystoles were demonstrated by ECG in 11. Eight had depressive episodes. It is likely that a further decrease in death-rate can be achieved only if the interval between onset of infarction and admission to an intensive care unit can be shortened.

Age Factors↗
PubMed1976

[Differentiation of infarct patterns in the computerized ECG and confirmation with clinical and coronarographic findings and cardiac catherization].

With a view to assessing the validity of ECG criteria for a personal computerization programme, definite external features have been introduced as controls to provide objective data on the patient's disease apart from the ECG picture. To analyse the reliability of this programme, the ECG infarction component of the following groups of patients was considered. 1) 84 ECGs of patients who have suffered myocardial infarction in the more or less distant past and the diagnosis of which was confirmed at the time by chemical and laboratory data. These patients were also submitted to angiocardiography. 2) 120 ECGs of patients who have suffered myocardial infarction in the comparatively recent past and diagnosis of which was confirmed by chemical and laboratory data. These patients were not submitted to angiocardiography. 3) 85 ECGs of definitely not coronaropathic patients in whom angiocardiography was performed. In these clinically well documented cases, computer diagnoses were well correlated with those of treating physicians. It should however be pointed out that in 18% of patients with myocardial infarction, the ECG did not confirm the lesion. In the control group also, ECG alterations were recorded in 18% cases. This is a high percentage because in numerous patients the presence of rheumatic or shunt valvulopathies or myocardiopathies was encountered.

Cardiac Catheterization↗
PubMed1975

[Atrial standstill (author's transl)].

A patient with a persistent form of atrial standstill is presented. The rarity of this arrhythmia is emphasized and the possible mechanisms involved and the diagnostic criteria are discussed.

Action Potentials↗