Search PubMed⌕ Search

Biomedical subjects

M Rotter

Publications and source records attributed to M Rotter.

At least 109 records · Page 6Linked to original sources
PubMed1979

[Yersinia enterocolitica--a still infrequently isolated pathogen in Austria (author's transl)].

Yersinia enterocolitica--recognized as a distinct taxonomic entity in 1964--belongs to the family of enterobacteriaceae. It has been isolated with increasing frequency from human and animal sources as well as from food and non-chlorinated water. Yersinia enterocolitica can produce enteritis in man, accompanied or followed in adults by erythema nodosum, arthralgia and/or acute arthritis. Rarely, septicaemia with a high mortality rate has been encountered. A cold-temperature enrichment method was used to examine 1135 faecal specimens; 11 were positive for Yersinia enterocolitica. Symptoms of enteritis were reported by all 8 patients whose faeces contained the bacterium; a brief description is given of the course of illness in each patient. Biochemical and serological properties of the isolates are discussed with special reference to some unusual results obtained with the commerical API-20 E system for identification of enterobacteriaceae when incubated overnight at 35 degrees C.

Adult↗
PubMed1979

[Has the rate of resistant staphylococci increased recently? (author's transl)].

In clinical material sent to the Clinic for Chemotherapy and the Institute of Hygiene of Vienna University between 1973 and 1978, Staphylococcus aureus was isolated from 0.9-2.3% and 4.2-11.9% respectively. The rate of resistance of the clinic material to oxacillin increased from 0.8 to 16.0%, to cephalothin from 3.3 to 30.5%, to gentamicin from 0.0 to 43.3%, and to erythromycin from 0.8 to 40.2%. In material sent to the Institute of Hygiene the rate of resistance to oxacillin was stable at 13.8%, and to cephalothin remained fairly constant at 2.4%. On the other hand, the rate of resistance to gentamicin increased from 0.5 to 48.5%, and to erythromycin from 19.8 to 56.9%. The reasons for these alarming increases in resistance and the conclusions which should be drawn are discussed.

Austria↗
PubMed1978

[Whole gut irrigation for large bowel preparation (author's transl)].

In a controlled trial whole gut irrigation (w.g.i.) was compared with elemental diet for 4 days as large bowel preparation. Cleansing effect, caloric intake and preparation time were significantly better in the first group (w.g.i.). In addition w.g.i. was routinely used studying 42 patients in an open trial which showed also a reliable cleansing effect and good practicability. At present the experience refers to a total of 163 preparations by means of w.g.i. In a further study the efficacy of w.g.i. for removal of blood from the intestinal tract in patients with bleeding esophageal varices was investigated. In 8 out of 11 cases a complete removal of the blood masses was obtained, 3 of these patients showed an improvement of the preexisting coma-stage.

Aged↗
PubMed1978

[Investigations on bloodcultures with three culture media containing Na-polyanetholsulfonate (aerobic iso- and hypertonic and anaerobic isotonic (author's transl)].

By using parallel culture techniques 330 blood specimens steming from patients of an intensive care unit were cultured simultanously in 3 media containing 0.05% Na-Poly-anetholsulfonate. Otherwise having an identical composition the media differed in the following respects : the isotonic medium A and the - by additional content of 10% sucrose - hypertonic medium B were used for aerobic culture. The anaerobic and isotonic medium C contained 2% Proteose-peptone, additionally. Without regards to the isolation of strictly anaerobic bacteria a total of 114 positive bloodcultures was obtained (tab. 1). The use of medium A resulted in 81 positive bloodcultures, that of medium B and C in 93 and 50, respectively (tab. 2). With the combined use of media A + B 103 positive bloodcultures would have been detected, with B + C 102, but with A + C 92 only (tab. 2). Of all 3 media the hypertonic medium B promotes bacterial growth most efficiently, but 13 strains grown in A did not grow in B (tab. 3), a fact that leads to the conclusion that isotonic as well as hypertonic aerobic media are employed in parallel advantageously. The effect of the hypertonic medium was expressed most distinctly with the isolation of grampositive bacteria (tab. 4). As during the period of this investigation the intensive care unit was afflicted by an outbreak of Serratia marcescens the predominance of this species among the other isolated microbes (tab. 5) is not surprising. While no difference of detection-time was observed between A and B, microbial growth in medium C seemed to be slower (fig). It was concluded that the use of 3 media, aerobic isotonic and hypertonic as well as anaerobic isotonic should be employed whenever possible.

Anaerobiosis↗
PubMed1978

[Effect of portocaval shunt and arterialization of the liver on antibodies to Escherichia coli in patients with cirrhosis of the liver (author's transl)].

The effect of a portocaval shunt with and without portal arterialization of the liver on serum immunoglobulin concentrations and on the incidence of antibodies to 8 different serotypes of Escherichia coli was studied in 29 patients with cirrhosis of the liver. Compared with healthy controls, the serum concentrations of IgG, IgA and IgM were significantly elevated in cirrhotic patients. No difference in immunoglobulin concentrations could be observed between shunted and arterialized cirrhotics. The incidence of E. coli antibodies was significantly higher in patients with cirrhosis of the liver, showing a further increase in patients with portocaval shunt operations. Portal arterialization of the liver after portocaval shunting did prevent this additional increase, presumably by restoring the antigen clearing capacity of the cirrhotic liver, thus avoiding an additional stimulation of the antigen response after the portocaval shunt. The quantitative contribution of E. coli antibodies to the hyperimmunoglobulinemia of patients with cirrhosis of the liver seems to be of little significance. The results of this study underline the significance of the portal hepatic blood flow for the function of the reticulo-endothelial system of the liver.

Antibodies, Bacterial↗
PubMed1978

[Evaluation of procedures for hygienic disinfection of hands: comparison of two methods, for artificially contaminating hands and use of an automatic colony-counter (author's transl)].

Testing procedures for the Hygienic Disinfection of hands, two kinds of artificial contamination have been compared: Application of one drop of bacterial suspension onto each fingertip as described in the DGHM-"Richtlinien" and immersion of hands as described in our previous papers. When using our method, fingertips released higher numbers of testbacteria. Since pre- as well as post-values were elevated, disinfection-tests applying one of each contamination method resulted in log10 reductions which did not differ significantly. Using an automated scanning colony counter we were able to save 2/3 of time otherwise required for manual counts. Up to 10(2) colonies per plate manual counts were practically identical with the counters results, whereas with higher colony-numbers the counter showed an increasing tendency to under-estimate the numbers of colonies. Since colonies of skin-staphylococci are not recognised by the automated counter, we cultured our rinsing-fluids on nutrient agar containing 0.05% of sodium-desoxycholate in order to inhibit the autochthonous skin flora. This supplement influenced the colony counts of our test-bacterium to a very minor degree only, the reduction factors were not at all altered.

Bacteriological Techniques↗
PubMed1978

[Increasing incidence of Serratia marcescens bacteraemia in intensive care patients (author's transl)].

The incidence of serratia marcescens in an intensive care unit was investigated in course of several years. After a trial of Cephalosporin-Gentamycin prophylaxis, infection and death due to serratia rose dramatically. The significance of decreased resistance to infection, hygiene regimes as well as mode of administration of antibiotics is related to selection of this rare microorganism causing septicemia.

Adolescent↗
PubMed1978

[Investigations on the antibacterial activity of fosfomycin on DST-agar (author's transl)].

Since in some European countries the activity of antimicrobial substances are assessed on Disc-Sensitivity-Test (DST)-agar, routinely, it has been investigated whether and under which conditions Fosfomycin might be tested on DST-agar with the agar-dilution and -diffusion technique. A total of 320 strains representing 7 species were used as teststrains, and Mueller-Hinton (MH)-agar was employed as reference medium. On DST-agar the MIC-values were increased for some genera like Klebsiella by a factor of 128, a fact that was explained by the sixfold concentration of antagonistically acting Phosphate in DST-agar. Addition of glucose-6-phosphate (G-6-P) enhanced the sensitivity towards Fosfomycin only with some species. As the endpoint of growth is difficult to determine on DST-agar its use for agar-dilution tests with Fosfomycin can not be recommended. For agar-diffusion tests, however, this medium may be used provided that a thin film of growth or single colonies within the inhibition zones are being disregarded and that the disc content is increased from 50 microgram as being usual at present to 100 or 200 microgram. Diameters of inhibition zones that were obtained with such discs on DST-agar correlated very well (r = --0,89 und --0,93, respectively) with MIC-values derived from agar-dilution tests on MH-agar (+ G-6-P). With discs containing only 50 microgram no inhibition zones are formed by strains that should still be classified as "sensitive" (MIC: 8--32 microgram/ml).

Agar↗
PubMed1977

A simplified one-step procedure for the simultaneous determination of complement receptor lymphocytes and lymphocytes with membrane-bound immunoglobulins.

A simple one-step procedure for the demonstration of complement receptor lymphocytes (CRL) with complement-coated bacteria (BC) as indicator particles is described. With this assay the percentage of CRL in normal peripheral blood ranged between 6 and 21% (mean 11%). In a separately performed combined assay for lymphocytes with membrane-bound immunoglobulins (M-Ig) and lymphocytes, which form rosettes with complement-coated bacteria (BC-RFC), four different fractions of lymphocytes could be detected: (M-Ig+-BC+, M-Ig+-BC-,M-Ig--BC+, M-Ig--BC-). These results suggest that the subpopulation of lymphocytes with complement receptor sites overlaps with, but is not totally identical with the lymphocyte subpopulation bearing Ig on the surface.

Adult↗
PubMed1977

[Microbiological diagnosis of septicemia (author's transl)].

To detect microorganisms in the blood it is necessary not only that the microbiologist uses reliable methods, but also that the clinician takes a sufficient number of blood samples at the right point in time using a correct method for drawing the blood. The best results are obtained if the blood sample is transferred to the culture media at the bedside. The media should contain anticoagulants, osmosis stabilizers and preparations to neutralize the microbial action of the blood (caused by intrinsic and extrinsic factors). Up until now sodium polyanetholsulfonate ("Liquoid") has proved to be the most suitable additive. The procedure used for blood culturing must enable growth of aerobes, anaerobes and microbes with cell-wall damage. Today, modern methods such as radiometry, impedance measurement and microcalorimetry are used or are in the process of being developed which facilitate screening for positive cultures. Antigens, cell-wall constituents and metabolites of bacteria and fungi present in the blood stream can be detected by means of counter-immunoelectrophoresis, the Limulus test and gas chromatography, without culturing being necessary. Concentration techniques such as filtration and centrifugation are also being refined to enable a more reliable and earlier detection of septicemia.

Adult↗
PubMed1977

[Usability of three alcohols for a standard disinfection method to be employed for the evaluation of procedures for the hygienic disinfection of hands (author's transl)].

Following a former suggestion (4) always to evaluate, the efficacy of procedures for Hygienic disinfection of hands in comparison with the results of a certain standard disinfection method, Ethanol, iso- and n-Propanol were tested in various concentrations and for various times of action on their usability in such a standard method. The disinfecting power was dependent upon (i) the alcohol (Ethanol less than iso-Propanol less than n-Propanol), (ii) the concentration (Ethanol: 60 less than 70 less than 80% ml/ml, iso-Propanol: 50 less than 60 less than 70, n-Propanol: 40 less than 50 less than 60 = 70) and (iii) the time of action (0,5 less than 1 less than 2 min). n-Propanol proved to be the fastest acting disinfectant. However, as standard disinfection method iso-Propanol (60% ml/ml) being used for 1 min has been proposed. Furthermore, the following results that have been obtained also in former investigations (8) could be confirmed: (i) there is no systematic difference between the release of test-bacteria from the fingertips of right and left hands of test-persons (ii). The efficacy of procedures for Hygienic disinfection of hands is besides other factors influenced by the testpersons. This factor may be eliminated by using the same testpersons for both, the disinfection procedure under investigation and the standard method. The results of both may, then, be related to each other and the efficacy of the former may be evaluated in comparison to the latter.

1-Propanol↗
PubMed1977

[Testmethod for the evaluation of procedures for the hygienic disinfection of hands. Part 1: Discription of testmethod (author's transl)].

A detailed test design for the evaluation of procedures for the hygienic disinfection of hands is described together with suitable methods for statistical analysis of the results. In principle the release of testbacteria from the finger-tips of artificially contaminated hands is measured before and after disinfection. A procedure under test is accepted if it is not less effective than a standard-disinfection procedure. As such the disinfection with iso-propanol 60% (ml/ml) applied through 1 min was agreed upon. This standard procedure is to be performed the same day with the same day with the same testpersons and under identical environmental conditions prior to the procedure under test.

1-Propanol↗