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Biomedical subjects

M Rothmund

Publications and source records attributed to M Rothmund.

At least 253 records · Page 14Linked to original sources
PubMed1987

The effect of oxotremorine on in-vitro secretion of parathyroid hormone.

The effect of the muscarine-like acting parasympathomimetic drug oxotremorine and its antagonist atropine on PTH release was tested using single cell suspensions obtained from a) primary parathyroid adenomas, and b) secondary hyperplastic parathyroid tissue from patients undergoing chronic hemodialysis. For both cell-types we found a dose-dependent suppression of PTH release with oxotremorine. The suppressive activity could be antagonized with atropine, suggesting that parasympathomimetic nerval impulses play a suppressive role in the regulation of PTH release. Muscarine-receptors appear to be responsible for this mechanism.

Adenoma↗
PubMed1987

[Crohn disease and squamous cell carcinoma of the anorectal transition].

Squamous cell carcinoma of the anorectal junction originated from anal fistulas in two female patients with Crohn's disease which have been suffering from anal and rectovaginal fistulas for many years. In addition one tumour showed paraneoplastic parathyroid hormone synthesis. This findings point out Crohn's disease as a premalignant lesion. Chronic fistulas associated with Crohn's disease must be followed up carefully by clinical examination, endoscopy and biopsy. To prevent malignancy and to cure the anal region and the origin of such fistulas, resective surgical technics should be taken into consideration early.

Adult↗
PubMed1987

[Reoperations in persistent and recurrent secondary hyperparathyroidism].

Forty-seven reoperations for persistent or recurrent secondary hyperparathyroidism (HPT) in 29 patients are reported. The patients are part of a group of 181 patients operated on for secondary HPT between 1975 to 1986. Most reoperations were performed in the neck (26 operations in 17 patients). The main cause were remaining parathyroid glands in the mediastinum (9 patients), followed by normally localized glands in the neck, which had not been discovered during previous operations. In 7 patients a hyperplasia of the autografted tissue was the reason for recurrent disease. They were treated by resection of the autograft. In 10 other patients autologous cryopreserved parathyroid tissue was reimplanted because of persistent postoperative hypoparathyroidism.

Adolescent↗
PubMed1986

[Regional chemotherapy of liver metastases of colorectal carcinoma using implanted gas pressure pumps. Results of a prospective phase II study].

22 patients with liver metastases of colorectal carcinomas were given floxuridine infusions into the hepatic artery using a totally implantable pump system (Infusaid, model 400). The course of the disease was compared to that of 22 untreated controls matched for age, sex, extent of metastatic disease and performance status. No therapy-related mortality was observed in the treatment group, morbidity was 37%. Response rate was 77% based on CEA decrease by more than 33% after 6 months, and 64% related to a more than 50% reduction in metastasis size. Median survival time was 10 months in the control group as compared to 26 months in the therapy group; the one-year survival rate was 37.5% and 88%, respectively.

Adult↗
PubMed1986

[Surgical indications in acute pancreatitis].

The indication for surgery in acute hemorrhagic necrotizing pancreatitis (AHNP) depends on the severity of the disease and the clinical course. Both factors must be determined daily, based on clinical and laboratory data. CAT-scan does not contribute much to indication. An early operation is necessary if despite an optimal intensive care septic symptoms and signs persist and renal and respiratory failure occur. Surgery is indicated 2-3 weeks after onset of AHNP if septic complications (re)-occur. A close follow up is mandatory.

Abscess↗
PubMed1986

The secretion of parathyroid hormone and its fragments from dispersed cells of adenomatous parathyroid tissue at different calcium concentrations.

The effect of varying calcium concentrations on PTH release from dispersed cells of adenomatous parathyroid tissues (n = 15) was studied. During high calcium concentrations PTH secretion was inhibited up to 62.5% as compared to low calcium concentrations. There was no correlation between iPTH secretion rate and suppressibility. Each adenoma had a different iPTH secretion rate. Three adenomas showed a high suppressibility (28.0%, 53.8%, and 62.5%). The supernates of their media were chromatographed and examined by midregion and C-terminal specific PTH-RIAs. Carboxyl-terminal PTH fragments were found to be released by two adenomas. There was no evidence for the release of midregion PTH fragments. Comparison of incubation media revealed that the adenoma with the lowest suppressibility released the highest amount of intact PTH (per 100,000 cells/ml). This adenoma exclusively secreted intact PTH, whereas PTH fragments were only released from two adenomas with higher suppressibility. In these media the ratio of PTH fragments to intact PTH increased with the calcium content of the media. The ratio was also dependent on the suppressibility of the adenomas; i.e. the ratio was greater when the suppressibility was higher. This suggests that in adenomas the intracellular hormone degradation is responsible for the degree of PTH suppression. As the suppressibility of PTH secretion is reduced in hyperfunctioning parathyroid tissue, diminished intracellular hormone degradation seems to be involved in the pathogenesis of primary hyperparathyroidism.

Adenoma↗
PubMed1986

[Simplified procedure for cold preservation of human parathyroid gland tissue for replantation].

A simplified technique for cryopreservation of human parathyroid tissue is described. It allows to achieve the same quality of tissue than using the programmable expensive freezing method which is usually employed up to now. Cryovials are placed in a methanol bath. The temperature is decreased by -1 degree C/min down to -25 degrees C, thereafter by -10 degrees C/min down to -70 degrees C by repeated adding of cryo ice-cubes into the methanol. Thereafter the vials are stored in LN2. The temperature lowering is controlled by a stop watch and a thermometer in a reference vial. This simple and cheap technique may allow a more general application of cryopreservation in surgery of hyperparathyroidism.

Cell Survival↗
PubMed1986

[Autotransplantation of cryopreserved human parathyroid tissue].

Replantation of autologous cryopreserved parathyroid tissue is a procedure well established in surgery of hyperparathyroidism. It has proved to be a good approach to successful surgical treatment of hypocalcaemia in the wake of parathyroid operations. Cryopreservation of parathyroid tissue is indicated in cases of subtotal parathyroidectomy, total parathyroidectomy with autotransplantation of fresh tissue, re-operation for persistent or recurrent hyperparathyroidism, and surgery for hyperparathyroidism following thyroidectomy.

Freezing↗
PubMed1986

The effect of cryopreservation on hormone secretion in vitro and morphology of human parathyroid tissue.

The hormone secretion from human parathyroid tissue autografted after cryopreservation can fail frequently. In this study we examined the effects of the cryopreservation procedure on parathyroid hormone secretion in vitro and the viability of parathyroid cells to identify possible reasons for graft failure. Cryopreservation did not affect quantitative parathyroid hormone release from single-cell suspensions or its calcium-and magnesium-dependent regulatory mechanisms. However, morphometric analysis of thawed 1 mm3 graft particles showed various degrees of necrosis when compared with fresh grafts. Thus partial necrosis of cryopreserved tissue appears to contribute to poor transplantation results compared with immediate replantation of fresh parathyroid tissue. This limitation can be overcome by increasing the number of frozen graft particles used for autotransplantation. The percentage of viable cells should be accounted for by morphometric analysis and this factor used when calculating the number of graft particles employed.

Cell Survival↗