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Biomedical subjects

M Rossignol

Publications and source records attributed to M Rossignol.

At least 91 records · Page 5Linked to original sources
PubMed1996

Diagnostic accuracy of technologies used in low back pain assessment. Thermography, triaxial dynamometry, spinoscopy, and clinical examination.

STUDY DESIGN: A prospective blind study compared three new technologies to assess back pain. OBJECTIVE: To assess the diagnostic accuracy and comparability of thermography, triaxial dynamometry, and spinoscopy in the assessment of recent onset work-related low back pain. SUMMARY OF BACKGROUND DATA: The role of these technologies in assessing patients with low back pain is unproved. METHODS: Forty-one patients with low back pain and 46 control subjects were assessed by each technology and by two clinical examiners blind to clinical status. Twenty patients were trained to simulate a healthy back without low back pain, and 50% of the control subjects were trained to simulate the presence of a low back pain disorder. Each technology was interpreted on two occasions by each of two readers. RESULTS: Thermography performed significantly worse than did triaxial dynamometry, spinoscopy, and clinical examination. The diagnostic accuracy of the last three was similar, and inter-rater comparability did not differ significantly. Among simulators, the diagnostic accuracy of triaxial dynamometry and spinoscopy was significantly higher than that of clinical examination, although considerable inaccuracy remained in assessing individual subjects. CONCLUSIONS: The diagnostic accuracy of thermography in recent onset low back pain does not support its use. Among those simulating normality or low back pain, triaxial dynamometry and spinoscopy have greater diagnostic accuracy than does a single clinical evaluation. However, for an individual, the inaccuracy that remains limits the use of triaxial dynamometry or spinoscopy for diagnosis in recent onset low back pain.

Adult↗
PubMed1996

MAT1, cdk7 and cyclin H form a kinase complex which is UV light-sensitive upon association with TFIIH.

MAT1, cyclin H and cdk7 are part of TFIIH, a class II transcription factor which possesses numerous subunits of which several have been shown to be involved in processes other than transcription. Two of them, XPD (ERCC2) and XPB (ERCC3), are helicases involved in nucleotide excision repair (NER), whereas cdk7, cyclin H and MAT1 are thought to participate in cell cycle regulation. MAT1, cyclin H and cdk7 exist as a ternary complex either free or associated with TFIIH from which the latter can be dissociated at high salt concentration. MAT1 is strongly associated with cdk7 and cyclin H. Although not strictly required for the formation and activity of the complex, it stimulates its kinase activity. The kinase activity of TFIIH, which is constant during the cell cycle, is reduced after UV light irradiation.

Amino Acid Sequence↗
PubMed1996

Auxin-induced differential sensitivity of the H+-ATPase in plasma membrane subfractions from tobacco cells.

Plasma membrane vesicles form suspension cultured tobacco cells were fractionated by counter-current phase partitioning. The subfractions exhibited identical biochemical characteristics and differed mainly by their sidedness. In addition, fluorescence polarization experiments indicated slight fluidity or order differences. These characteristics remained unchanged when cells were treated with auxin, prior to membrane purification. The sensitivity to auxin of the H+-ATPase catalysed proton translocation was investigated in plasma membrane subfractions. Identical sensitivity was measured in all subfractions from control cells. After auxin treatment of cells, a dramatic increase in the sensitivity was observed in one subfraction. Results are discussed in relation to the recent report of an auxin-induced clustering of the putative auxin-receptor (Diekmann et al., Proc. Natl. Acd. Sci USA 92, 3425-3429, 1995).

Cell Fractionation↗
PubMed1996

Renal effects of low-dose dopamine in patients with sepsis syndrome or septic shock treated with catecholamines.

OBJECTIVE: To evaluate the renal effects of low-dose dopamine in patients with sepsis syndrome or septic shock treated with catecholamines. DESIGN: Prospective, clinical study using sequential periods. SETTING: A 12-bed surgical intensive care unit in a university hospital. PATIENTS: 14 patients with sepsis syndrome and 15 patients with septic shock treated with exogenous catecholamines were studied. They had no diuretic treatment. INTERVENTION: Two periods of 2 h each with and without 2 micrograms.kg-1.min-1 of dopamine infusion. Hemodynamic and renal data were obtained at the end of each period. Measurements were repeated after 48 h of dopamine infusion in patients with sepsis syndrome. All data were evaluated by the Wilcoxon rank test. MEASUREMENTS AND RESULTS: In patients with sepsis syndrome, diuresis and creatinine clearance increased significantly by 100% and 60%, respectively, during low-dose dopamine infusion without any change in systemic hemodynamics. The renal response to dopamine decreased significantly after 48 h of dopamine infusion (P < 0.01). In patients with septic shock treated with catecholamines, no variation of either systemic hemodynamics or renal function was noted during low-dose dopamine infusion. CONCLUSION: The renal effects of low-dose dopamine in patients with sepsis syndrome decrease with time. No renal effect of low-dose dopamine was observed in patients with septic shock treated with catecholamines. These findings suggest a desensitization of renal dopaminergic receptors.

Adult↗
PubMed1996

Back school in a first episode of compensated acute low back pain: a clinical trial to assess efficacy and prevent relapse.

OBJECTIVE: To assess the efficacy of a back school program for patients with a first episode of acute work-related low back pain requiring compensation. DESIGN: A randomized single-blind controlled trial. SETTING: A private physiatrics outpatient clinic. PATIENTS: The mean duration of low back pain was 15 days. INTERVENTION: Eligible patients were randomized to a standard treatment program that included daily physiotherapy (n = 86) or the same program with the addition of back school (n = 82). The back school program consisted of three 90-minute sessions given by a single trained instructor at 0, 1, and 8 weeks. MAIN OUTCOME MEASURES: The primary outcomes were the time off work for the presenting episode of back pain and the number and duration of recurrences in the year following the study onset. Secondary outcomes included the level of pain, spinal mobility, active straight-leg raising, and functional disability assessed by the Oswestry and Roland-Morris scales. RESULTS: Those randomized to the back school group gained significantly more knowledge, based on the multiple choice examination (p = .0001) and performed the exercise program significantly better (p = .0001) than the standard care group. There were no differences between the two treatment groups for either of the primary outcomes. The median time to return to work from randomization was 33 days for both the back school and the standard care groups (p = .48). The number of compensated recurrences of low back pain over 1 year was similar (back school = 14, standard care = 10, p = .16), as was the median duration of these episodes (back school = 25 days, standard care = 70 days, p = .21). There were no significant differences favoring the back school group for any of the secondary outcomes at the posttreatment, 6-month, or 12-month assessments. CONCLUSION: A back school intervention in addition to standard care resulted in no reduction in the time to return to work or the number or duration of recurrences of low back pain requiring compensation over a period of one year.

Absenteeism↗
PubMed1996

Cloning of a cDNA encoding a developmentally regulated 22 kDa polypeptide from tobacco leaf plasma membrane.

A polypeptide doublet (P18-P19, ca 22 kDa, pI 4.5) has been shown to accumulate in tobacco leaf plasma membrane in a development-dependent way, under constant environmental conditions. P18 and P19 were purified by 2D-PAGE and microsequenced. Microsequences revealed only small differences between the two polypeptides. A PCR-based cloning strategy identified a cDNA displaying a 591 bp ORF. The encoded polypeptide contained P19 specific microsequences. It was expressed in E. coli and a specific rabbit antiserum was raised. Western-blots confirmed its identification as P19. The accumulation pattern of hybridizable mRNA around the floral induction period was similar to that of P18 and P19. Searching of databases revealed no significant hits except unidentified plant ESTs. P18 and P19 are proposed as the first example of plant-specific and developmentally regulated plasma membrane proteins.

Amino Acid Sequence↗
PubMed1996

Mitochondrial intronic open reading frames in Podospora: mobility and consecutive exonic sequence variations.

The mitochondrial genome of 23 wild-type strains belonging to three different species of the filamentous fungus Podospora was examined. Among the 15 optional sequences identified are two intronic reading frames, nad1-i4-orf1 and cox1-i7-orf2. We show that the presence of these sequences was strictly correlated with tightly clustered nucleotide substitutions in the adjacent exon. This correlation applies to the presence or absence of closely related open reading frames (ORFs), found at the same genetic locations, in all the Pyrenomycete genera examined. The recent gain of these optional ORFs in the evolution of the genus Podospora probably account for such sequence differences. In the homoplasmic progeny from heteroplasmons constructed between Podospora strains differing by the presence of these optional ORFs, nad1-i4-orf1 and cox1-i7-orf2 appeared highly invasive. Sequence comparisons in the nad1-i4 intron of various strains of the Pyrenomycete family led us to propose a scenario of its evolution that includes several events of loss and gain of intronic ORFs. These results strongly reinforce the idea that group 1 intronic ORFs are mobile elements and that their transfer, and concomitant modification of the adjacent exon, could participate in the modular evolution of mitochondrial genomes.

DNA, Fungal↗
PubMed1996

Physician referral to physical therapy in a cohort of workers compensated for low back pain.

BACKGROUND AND PURPOSE: This study described the physical therapy referral of workers compensated for back injury; characterized physical therapy by duration and choice of therapeutic techniques; and compared workers who were and were not referred for physical therapy in terms of age, gender, diagnosis, and absence from work. SUBJECT: A cohort of 2,147 subjects were randomly selected from 54,401 workers compensated for back injuries in 1988. METHODS: Each subject was followed for 2 years from date of entry into the study. Data were obtained from the Quebec Worker's Compensation Board computerized files, medical files, and initial reports completed by physical therapists. RESULTS: Of the cohort, 389 subjects (18%) had received physical therapy after referral by their physician. Exercise, heat, ultrasound, back education, manipulation, and transcutaneous electrical nerve stimulation were the most frequently selected treatments. Implementation of physical therapy within 1 month of back injury had a strong protective effect on return to work within 60 days. Female gender and presence of a specific diagnosis were predictors for greater than 60 days' absence. CONCLUSION AND DISCUSSION: This study demonstrated that physicians request physical therapy services based on certain patient characteristics. Patients who were referred earlier tended to return to work sooner than those who were referred later, which indicates that timing of physical therapy is an important factor in the rehabilitation of workers with low back pain.

Absenteeism↗
PubMed1996

Evaluation of the utility of spirometry in a regional public health screening program for workers exposed to welding fumes.

The objective of this study was to determine the usefulness of spirometry in the screening of chronic pulmonary problems related to exposure to welding fumes, in a regional public health-based screening program. Pulmonary questionnaires and spirometric tests were administered twice at 5-year intervals on 229 welders/cutters (mean age, 39.9 years from 31 metal manufacturing plants. The baseline mean value at the first test for the forced expiratory volume in one second (FEV1) was 102% of predicted, the forced vital capacity (FVC) was 107%, and the FEV1/FVC ratio was 79%. The mean annual changes in the three spirometric indicators were -47 mL, -46 mL, and -0.3%, respectively. The usefulness of spirometry was assessed by looking at the relationship between exposure to welding fumes and changes in spirometric indices, in multivariate linear regression analysis, accounting for age, height, and smoking. A high lifetime exposure to welding fumes was associated with better lung functions in the cross-sectional approach (healthy worker effect) but not in the longitudinal approach, where no association was found. We concluded that the public health regional application of this screening program generated too many sources of variation for spirometry to fulfill the objective of early detection of pulmonary function decline related to exposure to welding fumes. Based on these observations, we recommend that maintaining questionnaire screening, with spirometry reserved as a second-line diagnostic intervention.

Adult↗
PubMed1995

The prognostic consequences in the making of the initial medical diagnosis of work-related back injuries.

STUDY DESIGN: A cohort of 1848 workers, representative of all sectors of industry, who were compensated for a low back injury in 1988 but not in the previous 2 years, was followed over 24 months. OBJECTIVES: To determine the prognostic value of the physician's initial diagnosis of back problems. SUMMARY OF BACKGROUND DATA: In the absence of a standardized classification of diagnoses of back pain, this study aimed to provide an element of validity to a classification previously proposed that consists of "specific" and "nonspecific" back pain. METHODS: Medical charts were reviewed at the Quebec Worker's Compensation Board to extract the diagnosis made by the treating physicians within 7 days of the first day of absence from work. Diagnoses were categorized into "specific" (lesions of vertebrae and discs) and "nonspecific" (pain, sprains, and strains). The history of compensated work absence for low back pain in the following 24 months was obtained. RESULTS: A specific diagnosis was found in 8.9% (165) of the workers, accounting for 31.0% of the patients who accumulated 6 months or more of absence in 2 years. Increasing age and daily amount of compensation also were associated with an increased risk of chronicity. CONCLUSIONS: The physician's initial diagnosis was highly associated with the risk of chronicity. The explanation for this result is complex, involving the nature of the underlying lesion as well as the impact of the diagnosis "label" on the worker and on the physician-patient relationship.

Absenteeism↗
PubMed1995

A retrospective comparison of spinal and general anesthesia for vaginal hysterectomy: a time analysis.

The authors sought to compare time efficiency of spinal versus general anesthesia. The charts of 106 consecutive patients who had undergone a vaginal hysterectomy were analyzed. This analysis divided the patients into three groups: Group 1, spinal anesthesia; Group 2, general anesthesia; Group 3, spinal anesthesia with subsequent general anesthesia. The perioperative time course was divided into six intervals from entry into the operating room to discharge from the postanesthesia care unit (PACU). Total time was calculated by adding the six intervals. There were 85 patients in Group 1, 17 patients in Group 2, and 4 patients in Group 3. The mean times for surgical readiness once the anesthesiologist was present for Group 1, Group 2, and Group 3 were 21.4 +/- 7.3, 21.4 +/- 6.0, and 25.0 +/- 5.8 min, respectively. The total time for the three groups was 278.3 +/- 72.0, 245.9 +/- 23.1, and 295.0 +/- 101.2 min, respectively (P < 0.01 Group 1 vs Group 2). The difference in total time between Groups 1 and 2 was accounted for mainly by the stay in the PACU. This study concludes that there is no difference in the efficiency of operating room time use between spinal and general anesthesia.

Aged↗
PubMed1993

Indoleacetic acid pretreatment of tobacco plants in vivo increases the in vitro sensitivity to auxin of the plasma membrane H(+)-ATPase from leaves and modifies the polypeptide composition of the membrane.

The sensitivity to auxin of the H(+)-ATPase-mediated proton translocation was investigated in vitro using purified plasma membrane vesicles from tobacco leaves. In vivo pretreatment of tobacco plants with auxin promotes a 100-fold increase of the in vitro sensitivity to auxin. This effect is specific for biologically active auxins and is dose- and time-dependent. In addition, pretreatment with auxin induces the accumulation of several polypeptides in the plasma membrane. These polypeptides constitute the first set of hormone-responsive polypeptides evidenced in plant membranes.

Cell Membrane↗
PubMed1993

Comparison of spinal health indicators in predicting spinal status in a 1-year longitudinal study.

A 1-year longitudinal study was performed to assess and compare the predictive qualities of spinal health indicators (excluding the cervical spine) among aircraft assembly workers having at least 1 year seniority in a large assembly plant. Ten health indicators were compared to determine their sensitivity and predictive power with regard to back compensation and absenteeism in 12 months follow-up, and the presence of any limitation at work due to the back, or symptoms to the back at the end of the interval. The initial response to a self-administered questionnaire was obtained from 269 male workers, of whom 205 (76.2%) completed the follow-up questionnaire. Initial prevalence of symptoms to the back was 42.3%, limitation in performing at work was 28.1%, consultation of a health professional was 7.3%, and a history of compensation for the back (ever) was reported by 30.4%. During the year of follow-up, 16 (6%) of the 269 workers initially enrolled were granted 17 compensated episodes. Of the 205 workers who responded to the follow-up, 33 (16.1%) have been absent from work (with or without compensation) because of their back. The presence, at the beginning of the study, of a limitation in performing at work or in activities of daily living and a history of compensation (ever) were the three indicators independently associated with the occurrence of compensation or absenteeism (total work disability) related to a back problem during the following year.(ABSTRACT TRUNCATED AT 250 WORDS)

Absenteeism↗
PubMed1993

Fatal occupational injury rates: Quebec, 1981 through 1988.

OBJECTIVES: The purpose of the study was to estimate the death rates from occupational injuries in the province of Quebec for the period 1981 through 1988. METHODS: Worker's compensation files were used to ascertain numbers of deaths, which were used as the numerators in figuring the rates (it was estimated that these files reported 83% of the true number of deaths among men). Annual average estimates of the labor force were used as denominators. RESULTS: From 1981 through 1988, compensation was awarded for 1227 fatal work injuries. Among men (96% of the victims), rates declined from 1981 to 1988 (from 12.7 to 8.1 per 100,000); women's rates were stable (< or = 1.0 per 100,000). Compared with men, women had excess mortality from violent acts. Motor vehicle crashes accounted for 36% of all fatal injuries in 1984 and 1985 and declined thereafter. Fatal injury rates in forestry and mining rose to a 1987 maximum of 67.6 per 100,000. The construction sector had the largest number of deaths, despite a decline in rates from 1981 to 1988 (from 27.8 to 15.9 per 100,000). CONCLUSIONS: Except for construction and agriculture, reported fatal occupational injury rates in Quebec were similar to those in the United States. Motor vehicle crashes, falls, violent acts, and farming-related injuries were the most frequent causes of death.

Accidents, Occupational↗
PubMed1992

rol A modulates the sensitivity to auxin of the proton translocation catalyzed by the plasma membrane H(+)-ATPase in transformed tobacco.

In order to investigate the effect of the rol A gene product on the plasma membrane response to auxin, a clone of rol A-transformed tobacco was prepared. Auxin sensitivity was measured by the auxin concentration which induced the highest stimulation of H(+)-ATPase-mediated proton translocation on isolated plasma membrane vesicles. Both transformed and control plants exhibited identical auxin-sensitivity changes during vegetative and induction to flowering periods. However the sensitivity of flowering-transformed plants was 100-times higher than that of control plants. Consistent observations were also made when using rol A+B+C-transformed plants. These results suggest that the rol A gene product could either participate in or affect the reception/transduction pathway of auxin signal at the plasma membrane.

Biological Transport↗