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Biomedical subjects

M Rosa

Publications and source records attributed to M Rosa.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

Liver fatty acid-binding protein in two cases of human lipid storage.

FABPs in the various tissues play an important role in the intracellular fatty acid transport and metabolism. Reye's syndrome (RS) and multisystemic lipid storage (MLS) are human disorders characterized by a disturbance of lipid metabolism of unknown etiology. We investigated for the first time L-FABP in these two conditions. Affinity purified antibodies against chicken L-FABP were raised in rabbits, and found to cross-react specifically with partially purified human L-FABP. L-FABP content in liver samples of two patients with RS and MLS was investigated by immuno-histochemistry, SDS-PAGE and ELISA. L-FABP immuno-histochemistry showed increased reactivity in the liver of RS patient and normal reactivity in MLS liver. L-FABP increase in RS liver was confirmed by densitometry of SDS-PAGE and ELISA method. By these two methods the increase amounted to 180% and 199% (p less than 0.02), respectively, as compared to controls. A possible role of L-FABP in the pathogenesis of RS is discussed.

Adolescent↗
PubMedDate not supplied

Validating facemask use for gas exchange analysis in patients with congestive heart failure.

PURPOSE: Functional limitation, often associated with congestive heart failure (CHF), is most accurately assessed with measurement of peak VO2 (VO2peak) during exercise testing. VO2peak strongly predicts survival in CHF patients and is used to triage patients for cardiac transplantation. However, the traditional mouthpiece/noseclip equipment used for gas exchange analysis is often cumbersome and uncomfortable. Several alternative facemask designs tested previously in healthy cohorts were inferior to the mouthpiece for measuring VO2peak. In this study, a series of facemasks designed specifically for VO2peak testing (Hans-Rudolph) was employed. Although previously validated in healthy young adults, this apparatus has not been validated in patients with severe CHF. METHODS: The Hans-Rudolph facemasks were compared with the mouthpiece apparatus in 30 patients with severe CHF. Each subject completed a cardiopulmonary exercise test with each device. Twelve subjects were randomized to evaluate a possible training effect. To enhance recruitment, 18 additional subjects were recruited after they had completed a test with the mouthpiece. RESULTS: There was no evidence of a training effect and no differences in gas exchange measurements were found between the two devices. The weight-adjusted VO2peak measurements were 17.1 +/- 5.3 ml O2/kg/min for the mouthpiece group and 17.3 +/- 5.6 ml O2/kg/min for the mask group (P = 0.54). CONCLUSIONS: The facemask was shown to be equivalent to the mouthpiece for measuring VO2peak in CHF patients. Concerns of hypoventilation and decreased VO2peak associated with previous facemask designs were not substantiated. Since successful exercise testing depends on maximal exertion, providing a choice of equipment may facilitate cooperation without sacrificing accuracy.

Adult↗
PubMedDate not supplied

[Collaborative study on peritoneal dialysis (PD) as first dialysis treatment in an Italian region: 1994-2000].

BACKGROUND: In Lazio, only about 5% of uremic patients are on peritoneal dialysis (PD). The present study focuses on the parameters of PD selection, the treatment schedules, and the clinical outcomes of PD patients in the nine public facilities offering a PD program. A cohort of 249 first-time PD patients, from July 1, 1994 to December 31, 2000, was retrospectively considered. METHODS: For the enrollment of the patients, the Regional Dialysis Registry databank was consulted. On December 31, 2000, a systematic review of patient charts was performed to extract the reasons for the PD choice, details of PD schedule, peritonitis episodes, reasons for drop-out, and patient survival rates. In regard to technique success-defined as the probability of having a patient alive on PD-change of modality and death were considered as final events. In regard to patient survival, only death, even in the first 2 months after a shift to hemodialysis, was considered the end point. RESULT: The main PD selection reasons were patient and/or nephrologist preference in 90% of cases. One-hundred eighty-nine patients (76%) had been started on CAPD. During the follow-up, 38.2% dialysis schedules had been modified at least once. At the end of follow-up, 41.2% patients were on APD. The peritonitis rate was one episode per 30 patient-months (1 per 27 patient-months in CAPD; 1 per 37 patient-months in APD; p = 0.08). The technique success rate was 66.3% after 2 years and 49.8% after 3 years. The patient survival rate was 81.1% after 2 years and 68.7% after 3 years. CONCLUSIONS: Patients chose PD as a first dialysis treatment mainly because of reasons unrelated to their clinical status. The technique's success, patient mortality rates, and the peritonitis rate do not explain the low PD diffusion in the region. The peritonitis rate meets the target criteria for excellence recommended by the Italian Society of Nephrology. The observed outcomes may have been favored by the selection of motivated patients and by the increased use of APD.

Adult↗
PubMedDate not supplied

[Isolation of Streptococcus agalactiae in adults: serotypes, adhesiveness to epithelial cells].

Serotype distribution and adherence to epithelial cells (HeLa and Hep-2) were studied in 230 strains of Streptococcus agalactiae (GBS) isolated in adult patients with GBS infection or in asymptomatic carriers. In the population investigated, serotypes Ia and III predominated in the strains isolated from both carriers and patients. Adhesivity was significantly greater in strains isolated from the former, regardless of serotype. Thus in adults, serotype cannot be correlated with virulence of a given strain.

Adult↗
PubMedDate not supplied

[Attitudes, knowledge and methods of assistance used by general practitioners in Novara in treating drug addicts. Study of 100 doctors].

The study examines the relationship between the general practitioner and the drug addict and aims to evaluate some aspects of the work of the health service, above all with reference to the methods of assistance, attitudes and knowledge relating to the problem of treating drug addicts. A questionnaire was sent to 100 general practitioners in Novara; it consisted of 15 questions relation to the doctor's opinion of the drug addict, the methods and place of intervention, the doctor's judgement regarding his/her own preparation and function, and the frequency of contact with drug addicts. The findings show that the majority of doctors have a positive attitude towards the problem, but that there is also a widespread feeling of impotency, dislike, loneliness, disinformation, and in addiction a feeling of educational inadequacy on the subject.

Attitude of Health Personnel↗
PubMedDate not supplied

Cytologic changes simulating malignancy in thyrotoxic goiters treated with carbimazole.

The cytologic patterns in toxic goiters treated with carbimazole were studied. Most cases exhibited changes, especially the unusually large nuclei, that could be mistaken for malignancy in panoptic-stained material. Study of the nucleolar morphology by means of the toluidine blue stain, however, showed a clear difference from malignant nucleolar types, with the nucleoli displaying types as in the cells of benign euthyroid colloid goiters. No increased transcription activity was seen in the nuclei. Nucleolar staining is thus recommended to avoid diagnostic errors in these and similar atypical cases. The apparent cytostatic effect of carbimazole on thyroid cells is in accord with other reports in the literature.

Acid Phosphatase↗