Search PubMed⌕ Search

Biomedical subjects

M Ristić

Publications and source records attributed to M Ristić.

At least 19 recordsLinked to original sources
PubMed2005

[Off-pump myocardial revascularization (OPCAB) in patients with post-infarction unstabile angina, low ejection fraction and renal disfunction].

UNLABELLED: The purpose of this study is to present our experience in off-pump myocardial revascularization in patients with post-infarction unstable angina, left ventricular low ejection fraction and renal dysfunction. MATERIAL AND METHODS: From January 1998. until march 2002, at the Institute for Cardiovascular Diseases in Clinical Centre of Serbia, we have operated 20 patients with post infarction unstable angina, echocardiographicaly proved low ejection fraction (less than 30%) and renal dysfunction (Serum Creatinin 150 micrograms per liter) using this method. All patients were male and they were between 52 and 79 years old. Preoperative characteristics, surgical treatment and postoperative course are presented. RESULTS: There was no hospital mortality, as well as important morbidity. There was no worsening of the renal and myocardial function. Postoperative ICU stay was from 1 to 2 days. Postoperative hospital stay was between 3 and 7 days. CONCLUSION: Off-pump myocardial revascularization is safe and effective procedure in all patients with left ventricular low ejection fraction and renal dysfunction.

Aged↗
PubMed2004

The chemical composition of some Lauraceae essential oils and their antifungal activities.

The antifungal activity of Aniba rosaeodora, Laurus nobilis, Sassafras albidum and Cinnamomum zeylanicum essential oils were investigated against 17 micromycetes. Among the tested fungal species were food poisoning, spoilage fungi, plant and animal pathogens. In order to determine fungistatic and fungicidal concentrations (MIC and MFC) macrodilution and microdilution tests were used. Linalool was the main component in the essential oil of A. rosaeodora, while 1.8-cineole was dominant in L. nobilis. In sassafras essential oil safrole was the major component and in the oil of C. zeylanicum the main component was trans-cinnamaldehyde. The essential oil of cinnamon showed the strongest antifungal activity.

Antifungal Agents↗
PubMed2003

[Role of nitric oxide in treatment of acute lung injury after surgery with extracorporeal circulation].

Postoperative acute lung injury (ALI) compromises oxygen transfer across alveolar-capillary membrane with consecutive hypoxia, one of its indicators being reduction of oxygenation index PaO2/FiO2 below 40 kPa (300 mm Hg). Management of ALI includes different procedures like mechanical lung ventilation (MLV), drugs and others. One of the new possibilities for treatment of ALI is nitric oxide (NO) inhalation. The aim of this prospective study was to examine the role of NO inhalation in treatment of ALI. 14 patients with ALI developed immediately after operation with extracorporeal circulation (ECC) were included in the study. Group A (n = 8) inhaled NO and group B (n = 6) did not inhale NO during treatment of ALI. All other therapeutic measures were the same in both groups. The groups were similar in relation to demographic data, type of surgery and duration of ECC. PaO2/FiO2 was calculated before operation (T1), immediately after surgery (T2) and after lung recovery, when the need for MLV stopped (T3). The duration of MLV was also registered. PaO2/FiO2 (kPa) in referent times was in group A 54.9 +/- 1.6, 33.8 +/- 1.2 and 46.2 +/- 0.8 and in group B 52.2 +/- 1.1, 33.5 +/- 1.5 and 47.3 +/- 0.9, respectively. There was a statistically significant decrease of PaO2/FiO2 in T2 and T3 vs T1 in both groups (p < 0.05), while the difference between the groups was not statistically significant. The duration of MLV (h) in group B (28.5 +/- 1.6) was statistically significantly shorter than in group A (63.1 +/- 8.7) (p < 0.01). According to the results of this study we conclude that NO inhalation during ALI after surgery with ECC significantly reduces the duration of MVL and improves pulmonary recovery.

Administration, Inhalation↗
PubMed2003

[Interleukin-8 serum levels in patients with various types of open heart surgical procedures performed under extracorporeal circulation].

Serum concentrations of proinflammatory cytokine interleukin-8 (IL-8) in 15 patients with surgically revascularized myocardium by triple venous cardiopulmonary bypass (CPB x 3) and in 10 patients with implanted artificial aortic valves (AV) were measured. Average IL-8 concentrations in (CPB x 3) patients and in those with artificial aortic valve were 7.3 +/- 11.6 pg/mL 24 hours before surgery, i.e. 3.3 +/- 3.4 pg/mL; six hours after surgical procedure 32.7 +/- 71.4 pg/mL, i.e. 8.9 +/- 9.9 pg/mL; and 24 hours after surgery 10.9 +/- 9.7 pg/mL, i.e. 8.3 +/- 4.6 pg/mL. Extracorporeal circulation (ECC) caused significant increase of IL-8 serum concentration in both investigated groups six hours after surgery. Comparing preoperative values of the both groups, as well as those of 6 and 24 hours after surgery, no significant values of IL-8 were found. Various types of open heart surgical procedures had no influence on the extent of the production and secretion of proinflammatory IL-8 cytokine measured in patients during 24 hours after surgery.

Adult↗
PubMed2002

Composite conversion and temperature rise using a conventional, plasma arc, and an experimental blue LED curing unit.

The objective of this study was to evaluate the degree of conversion and temperature rise in three different composite materials when illuminated by an experimental light source [blue superbright light emitting diodes (LEDs)] and compared with plasma light and traditional photopolymerization unit. The degree of conversion and temperature rise were measured using Fourier transform infrared (FTIR) spectroscopy and digital multimeter, respectively. The results revealed significantly higher degree of conversion values in case of conventional curing than with other two light sources whereas temperature rise was significantly lower when blue LEDs and plasma light were used. There were great differences in light intensities between blue LEDs of only 9 mW cm-2 compared with plasma light of 1370 mW cm-2 and Elipar II of 560 mW cm-2. Better match of LED spectral distribution peak to camphorquinone absorption distribution peak probably explains much lower intensities used for similar photopolymerization effect like in the case of rapid plasma lamp curing.

Absorption↗
PubMed2002

Photopolymerization of composite resins with plasma light.

Everyday improvements in components and characteristics of composite materials have induced faster development of curing units. Besides standard halogen curing units and soft-start photopolymerization light sources, some experiments with argon and pulsed laser light and low intensity blue superbright light emitting diodes have been made. On the other hand, rapid polymerization with strong plasma light is also clinically applicable. The aim of this study was to measure the degree of conversion and temperature rise for three restorative composite materials: Tetric Ceram (Vivadent, Schaan, Liechtenstein), Pertac II (ESPE, Seefeld, Germany) and Z100 (3M Dental Products, St Paul, MN, USA) during polymerization with plasma light Apollo 95E (DMDS, Dental/Medical Diagnostic Systems, Fleury d'Aude, France) and compare it with the results of polymerization with a halogen curing unit, Elipar Trilight (ESPE, Seefeld, Germany). The results revealed the degree of conversion values in the case of polymerization with plasma light to be almost equal to those obtained by curing with the halogen curing unit, whereas the temperature rise was almost negligible.

Analysis of Variance↗
PubMed2001

Degree of conversion and temperature rise during polymerization of composite resin samples with blue diodes.

To ensure an adequate clinical composite filling light source for photopolymerization is of great importance. In everyday clinical conditions commonly used unit for polymerization of composite material is halogen curing unit. The development of new blue superbright light emitting diodes (LED) of 470 nm wavelengths comes as an alternative to standard halogen curing unit of 450-470 nm wavelengths. The purpose of this study was to compare the degree of conversion (DC) and temperature rise of four hybrid composite materials: Tetric Ceram, Pertac II, Valux Plus and Degufill Mineral during 40 s illumination with standard halogen curing unit Heliolux GTE of 600 mW cm(-2) intensity, Elipar Highlight soft-start curing unit of 100 mW cm(-2) (10 s) and 700 mW cm(-2) (30 s) intensity and 16 blue superbright LED of minimal intensity of 12 mW cm(-2) on the surface and 1 mm depth. The results revealed only a little bit higher DC values in case of polymerization with even 66 times stronger halogen curing units which showed twice higher temperature than blue diodes. Temperature and DC obtained are higher on the surface than on 1 mm depth regardless on the light source used.

Analysis of Variance↗
PubMed2001

[Comparative analysis in the use of natural and artificial vascular grafts on hematologic characteristics in patients].

Investigation of two groups of patients was performed. Both male and female patients, 34 to 69 years old, in which extracorporeal circulation was established in the course of open heart and ascendental aorta surgeries were included in the investigation. The first group consisted of 20 patients with surgically revascularized miokard by triple venous grafts, and the second included 20 patients with partial replacement of ascendental aorta using synthetic dacron graft. It was concluded that extracorporeal circulation leads to a significant decrease of platelet count and agreability after surgery, as well as decrease of red blood cell count and hemoglobin and hematocrit value. However, contact of circulating blood with the synthetic dacron graft surface, in surgical procedures on ascendental aorta, leads consequently to a significantly higher decrease of platelet count and agreability, with the significant decrease of red blood cell count and decrease of hemoglobin and hematocrit value. Based on the obtained results it is possible to plan requirements for allogenous blood/red blood cells which are significantly higher in patients undergoing partial replacement of ascendental aorta using artificial dacron graft compared with patients after surgical revascularization of miocard with triple venous graft.

Adult↗
PubMed2001

[Stability of myocardial perfusion during revascularization under propofol anesthesia].

During coronary artery bypass grafting surgery (CABGS) there are many factors which may disturb myocardial oxygen balance with consequent irreversible myocardial damage. Anesthesiologists are expected to use drugs with tendency to optimize hemodynamics and myocardial oxygen balance, making in that way conditions for preserving myocardial perfusion. In this study, performed on 19 patients undergoing CABGS under dominant propofol anesthesia, there were no signs of intraoperative ischemia manifested by statistically significant dislocation of ST segment in EKG leads D11, V5 and aVL.

Anesthesia, Intravenous↗
PubMed1999

Guaianolides from Centaurea nicolai: antifungal activity.

A new guaianolide, 3-deacetyl-9-O-acetylsalograviolide A, along with four known closely related lactones, salograviolide A, 9-O-acetylsalograviolide A, kandavanolide and salograviolide B were detected in the aerial parts of the flowering plant Centaurea nicolai. Antifungal tests performed on salograviolide A and its 9-O-acetyl and 3-O-deacetyl-9-O-acetyl derivatives revealed inhibitory activity against Aspergillus niger, A. ochraceus, Penicillium ochrochloron, Cladosporium cladosporoides, Fusarium tricinctum and Phomopsis helianthi. Neither of them was active against Trichoderma viride.

Antifungal Agents↗
PubMed1995

Contamination of broiler chicken's mash and litter with moulds, aflatoxins, ochratoxin A and zearalenone.

Contamination of broiler chicken's mash and litter with moulds, aflatoxin B1 (AB1), G1 (AG1), ochratoxin A (OA) and zearalenone (F-2 toxin) was examined on a broiler fattening farm. All broiler chicken's mash samples tested were contaminated with moulds. The highest total viable counts of moulds (3.3 x 10(5) per g) were found in broiler starter diet. Moulds isolated from mash were classified into 8 genera and 27 species. Many of them (Aspergillus spp., Fusarium spp., Penicillium spp.) are known as mycotoxin-producing moulds. Fusarium moniliforme was the dominant species in mash mycopopulations. Moulds were also found in all litter samples. The highest fungus count (80 x 10(5) per g) in litter was found at the beginning of fattening. Fungi isolated from litter samples belonged to 9 genera and 22 species. Aspergillus, Penicillium and Mucor were the most prevalent genera. None of the broiler chicken's mash and litter samples tested was contaminated either with AB1, AG1, OA or with F-2 toxin. F. moniliforme was zearalenone-negative also under laboratory conditions.

Aflatoxins↗
PubMed1993

The influence of inedible raw material rendering method on meat meal quality.

The dependence of meat meal quality on inedible raw material rendering method was studied. The conventional batch dry rendering method with screw press defatting and the semi-continuous wet rendering method with centrifugal defatting were employed for processing inedible raw material (76.5% soft offals, 15% industrial bones and 8.5% swine cadavers). Amino acid destruction in the protein component was higher with the conventional batch dry rendering method. The contents and biological activities of lysine, methionine and cystine show that the nutritive value of meat meals produced by the conventional batch dry rendering method is lower than that of meat meals obtained by the semi-continuous wet rendering method.

Animal Feed↗
PubMed1992

A stereological investigation of rat endocrine pancreas after a long-term low-protein diet.

Stereological analysis of rat endocrine pancreas was carried out after 4 months of low-protein intake. Thirty make Wistar rats, aged 2 months at the beginning of the experiment, were divided into three groups. The control group (NP) received a standard laboratory diet. The experimental group (LP) received a low-protein diet, and the pair-fed animals also received a standard laboratory diet, as the NP rats did, but restricted to the amounts consumed by the LP group. All groups were offered drinking water ad lib. Weibel's multipurpose lattice was used to determine the volume fraction of cells and numerical density of islets in the endocrine pancreas. The results showed that the long-term low-protein diet decreased the volume fraction of B-cells and increased that of A-cells. Numerical density of islets was significantly increased, whereas the absolute number was decreased. The mean number of B-cells per islet was found to be decreased and that of D-cells increased. The mean radius of the islets was decreased, as was the mean surface area.

Animals↗
PubMed1990

[In vitro culture of cells from patients with leukemia].

The development of cytological, cytochemical and immunological investigations of leukemic cells as well as new cytostatic drug combinations have led to great progress in the diagnosis and treatment of acute leukemia (AL). However, a heterogeneity in response to the same kind of therapy has been noted in patients with the same cytological type of the disease. This points out the importance of investigations of leukemic cells in culture which should provide more data on proliferation and reactivity of these cells. The population of tumor cells in heterogeneous in morphological and biochemical properties, in proliferative capacity and other characteristics. In cell culture it is possible to investigate the growth of cells and the effects of various agents on normal and malignant cells. In this study we investigated the growth of cells from bone marrow of patients with acute leukemias and acute phase of chronic leukemias. Cells from 17 patients were studied. The patients were from Internal Clinic A in Belgrade and the investigations were performed before treatment. Bone marrow cells were cultured in vitro on plastics and growth of the adherent cells was followed in the course of 2-3 weeks. The results show that bone marrow cells adhered and proliferated in 12/17 patients, there was low proliferation in 4/17 and none in 1/17. It is shown that in these conditions a successful growth of fibroblast-like shaped cells can be obtained with the bone marrow cells, but it is still not possible to correlate the course of the disease and the proliferation in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)

Humans↗
PubMed1990

[Hereditary factors in the etiology of chronic lymphocytic leukemia].

Leukocyte counts, hemoglobin concentration, PAS positivity index in lymphocytes, leukocyte alkaline phosphatase, karyotype, HLA phenotype as wall as the quality of the cellular and humoral immunity have been studied in 45 patients with chronic lymphocytic leukemia (CLL) and in 103 their closest relatives (sibs, parents). The aim was to detect the possible preleukemic condition in the relatives as a strong propensity towards developing has been previously established in families of patients with CLL. In the CLL patients our studies have confirmed the results of the determination of similar parameters by other authors, namely a higher percentage of PAS+ lymphocytes than normal together with a variety of humoral and cellular immunity disturbances. Regarding the morphological and cytochemical changes in lymphocytes in family members of the CLL patients a higher frequency of PAS+ lymphocytes have been observed: it has been established in over 10 per cent lymphocytes (normally 4-8 per cent) in 31 (30%) examinees and in 14 (13.5%) of these persons the percentage of PAS positivity was equal to that found in CLL patients. Cellular immunity examinations established that in one third of examined E rossete counts counts were lower and in 2 persons the degree of this decrease corresponded to the decrease observed in CLL patients. In one fifth of the examined family members T4:T8 ratio was reduced. In the 13 per cent of the examined M rossete counts were increased, a fact which supports the notion that immunity of B cells exists in relatives of CLL patients. The authors contend that the increased percentage of PAS+ lymphocytes and inadequate functional maturity of the T and B cells in a relatively large number of family members of the CLL patients, intimates that a hereditary disturbance in the lymphocyte make up and their role in the immunity pathways exists and could possibly represent one of the factors implicated in a high frequency of CLL in some families.

Aged↗
PubMed1990

[Morgagni's diaphragmatic hernia--case report].

A sucostosternal hernia (type Morgagni) is a rare diaphragmatic anomaly of congenital origin; in that sense are data that from 100 operated on diaphragmatic hernias, only three belong to this type. Although it is known that 20% of patients with congenital diaphragmatic hernia have other congenital anomalies, a coincidence of a Morgagni's hernia with heart anomalies, in available to use (domestic and foreign) references, there has not yet been reported. Besides a Morgagni's hernia, our patient also had an insufficiency of mitral and tricuspidal valves, atrium septum defect (sec.), pulmonary hypertension and Hiss' right branch block. Surgery was successful: the diaphragmatic defect (size: 7 cm. x 3 cm.) repaired, and the complex heart anomalies corrected in an extracorporeal circulation.

Adult↗