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Biomedical subjects

M Rieger

Publications and source records attributed to M Rieger.

90 records · Page 5Linked to original sources
PubMed1979

Induction of suppressor cell mechanism in antilymphocyte serum-induced skin allograft tolerance in mice.

In the B10.D2 leads to B10.D2(M504) strain combination (H-2D incompatibility), 20--40% of skin allografts survive for more than 100 days in ALS-treated recipients. Allograft tolerance in ALS-treated recipients could not be abolished by adoptively transferred normal or immune syngeneic spleen cells, but it could be adoptively transferred by spleen or lymph node cells to sublethally irradiated syngeneic mice. The suppressive activity of transferred cell population markedly declined after treatment with anti Thy-1.2 serum and complement. Cells with suppressor activity could be demonstrated by adoptive transfers as early as 5 days after skin grafting and ALS treatment. The results showed that the long-term allograft-promoting effect of ALS was caused not only by a decrease in the graft-rejection cell potential of the recipients (as demonstrated by other authors earlier) but also by the activation of a T cell-mediated suppressor mechanism.

Animals↗
PubMed1979

Immunoprofile studies in patients with pulmonary tuberculosis. III. Study of haemolytic complement in serum and phagocytic activity of blood neutrophils.

The total level of haemolytic complement in serum, and the phagocytic activity of blood neutrophils were found to be significantly increased in patients with active pulmonary tuberculosis prior to treatment. A relationship could not, however, be demonstrated between the elevation in the two studied values and the extent of tuberculous changes in the lungs of the patients.

Complement System Proteins↗
PubMed1979

Immunoprofile studies in patients with pulmonary tuberculosis. IV. Tuberculin skin reaction and test of inhibition of blood leukcoyte migration.

Study of the relationship existing between in vivo and in vitro correlates of cell immunity has revealed that there is no marked correlation between the magnitude of skin induration of the tuberculin skin test and the degree of inhibition of migration of blood leukocytes stimulated by PPD in patients with newly detected, bacteriologically confirmed pulmonary tuberculosis.

Cell Migration Inhibition↗
PubMed1978

[Cardiotoxicity in long term treatment with cytostatics (author's transl)].

During cytostatic combination treatment with five different anti-tumor agents two female patients suffered from cardiac failure which proved fatal. This was mainly due to doxorubicine (adriamycin) and daunorubicine. In an 8-year-old patient the cumulative dose was reached after 14 months of treatment (total dose of adriamycin 420 mg/m2, of daunorubicine 280 mg/m2) and in a 7-year-old patient after 16 months (total dose of adriamycin 480 mg/m2, of daunorubicine 280 mg/m2). Cardiac failure which occurred 12 months after the onset of treatment in a 12-year-old girl (total dose of adriamycin 310 mg/m2 and of daunorubicine 280 mg/m2) could be brought under control. The cumulative dose of adriamycin should be limited to 500 mg/m2 in children without risk factors.

Bone Neoplasms↗
PubMed1978

Evidence of suppresssor cell-mechanism of allograft tolerance induced by spleen extract and hydrocortisone.

The mechanism of allograft tolerance in mice induced by cell-free spleen extract in combination with hydrocortisone was investigated. HC potentiated tolerance induction when given after the spleen extract. When HC was given before, or during the first 2 weeks of antigenic treatment, it counteracted rather than potentiated the tolerance-inducing effect. Spleen or lymph node cells transferred from tolerant donors to untreated or sublethally irradiated syngeneic recipients suppressed the recipients' response to the particular alloantigens. The suppressive activity of lymphoid cells from tolerant donors was abolished when before their transfer the subpopulation of T cells was eliminated by means of anti Thy-1.2 serum and complement. This finding suggests that suppressor T cells may be involved in tolerance induced by spleen extract and hydrocortisone.

Animals↗
PubMed1977

The involvement of a suppressor mechanism in neonatally induced allograft tolerance in mice.

The possible involvement of a suppressor cell mechanism in neonatally induced allograft tolerance was tested in two different mouse-strain combinations. In an H-2 incompatible donor-recipient combination presented by the congeneic strains B10.D2 and B10.D2(M504), adoptive transfer of tolerance with 5 X 10(7) spleen cells to syngeneic sublethally (400 R) irradiated recipients was successful resulting in a markedly prolonged test graft survival or even take. The result of tests for negative tolerance (which should be suceptible to abolition by means of cells from normally reactive donors) did not indicate that neonatally induced tolerance across the given barrier rested on this principle. The suppressor mechanism seemed to be involved in neonatally induced allograft tolerance also in the other strain combination (B10-40NX).

Animals↗
PubMed1975

Allograft tolerance as in immunologically active process: analysis in a restricted H-2 incompatibility due to mutation (M504) in mouse strain B10.D2.

Cell-free spleen and liver extracts were used for attempted induction of allograft tolerance across a restricted H-2 barrier due to a chemically induced mutation in the B10.D2 mouse strain. When using mice of the latter strain as donors and the new congenic strain carrying the mutant H-2 hyaplotype as recipients, and with long-term administration of the cell-free antigens in adult life up to 100% yields (according to the dose of antigen) of permanently tolerant animals (particularly males) was obtained with spleen extract, but not with liver extract. This contrasts with a higher tolerance-inducing efficiency of liver extracts in various non-H-2 incompatibilities. In the reversed donor-recipient combination, neither spleen nor liver extracts gave positive results. The immune status of the permanently tolerant animals was analyzed and it was shown that with their bone marrow normal responsiveness can be conferred upon lethally irradiated syngeneic hosts whereas the specific allograft tolerance can be adoptively transferred to sublethally irradiated recipients by means of spleen or lymph node cells. It is concluded that in the present system the state of allograft tolerance is maintained by an immunologically active process of peripheral lymphocytes.

Animals↗
PubMedDate not supplied

Use of phthaloyl protecting group for the automated synthesis of 3'-[(hydroxypropyl)amino] and 3'-[(hydroxypropyl)triglycyl] oligonucleotide conjugates.

The chemical stability of oligonucleotides (ODNs) containing 3'-propanolamine was investigated. Invariably, all the ODNs synthesized from Fmoc-protected 3-aminopropane-1,2-diol-CPG support gave a mixture of three compounds at the end of automated synthesis as analyzed by denaturing PAGE and HPLC. On the basis of analytical procedures, these compounds were identified to be 3'-[N-acetyl-N-(hydroxypropyl)amino],3'-[(hydroxypropyl)amino], and 3'-hydroxyl ODNs. The instability of the amino protecting group under the synthesis conditions was responsible for this observed heterogeneity. In order to evaluate the stability, a comparative study on the chemical stability of the ODN containing amino-protecting groups such as [(9-fluorenylmethyl)oxy]carbonyl (Fmoc), trifluoroacetyl (TFA), and phthaloyl was undertaken. The results indicate that the phthaloyl group provided the best stability for the synthesis of 3' amine-modified ODNs, and the protecting group is cleaved and deprotected in concentrated ammonium hydroxide:40% aqueous methylamine, 1:1, for 5-10 min, at 56 degrees C. The 3'-hydroxypropyl)triglycyl] ODN conjugates were also synthesized from Fmoc- and phthaloyl-protected (hydroxypropyl)triglycine-CPG supports.

Base Sequence↗
PubMedDate not supplied

Bioabsorbable polyglyconate interference screw fixation in anterior cruciate ligament reconstruction: a prospective computed tomography-controlled study.

PURPOSE: It was the purpose of the study to evaluate a new polyglyconate bioabsorbable interference screw for graft fixation in anterior cruciate ligament (ACL) reconstruction. TYPE OF STUDY: Prospective randomized. MATERIALS AND METHODS: Forty patients who underwent endoscopic ACL reconstruction were included in the study and randomized intraoperatively. Group A consisted of 20 patients (6 women, 14 men; mean age, 29.6 years) who had femoral bone block fixation with a bioabsorbable interference screw and tibial fixation with a titanium interference screw. Group B included 20 patients (5 women, 15 men; mean age 29.6 years) who had fixation of both femoral and tibial bone blocks with titanium interference screws. There was no significant difference between the groups with regard to age, gender, height, weight, time from injury to surgery, activity level, and concomitant injuries. RESULTS: Clinical results (using IKDC, Lysholm, Tegner scores) of the 2 groups as well as instrumented laxity measurements (KT-1000) did not show significant (P >.05) differences at any stage of follow-up. No complications with respect to graft fixation could be found. Computed tomography scans, performed within the first postoperative week, at 6 weeks, and at 3, 6, 12, and 24 months postoperatively revealed a uniform picture for all patients within the groups, showing completed screw degradation at 12 months in group A. CONCLUSION: Polyglyconate interference screw fixation for patellar tendon grafts has not been found to be associated with increased clinical complications or significant osteolysis. It provided equivalent fixation and clinical results compared with titanium screws. However, replacement of the screw with bone did not take place for up to 3 years postoperatively.

Absorbable Implants↗
PubMedDate not supplied

Prognostic relevance of soluble interleukin-2 receptors in patients with ovarian tumors.

Soluble interleukin-2 receptors (sIL-2R) are measurable in the sera of patients with ovarian cancer and several other benign and malignant diseases. However, the function of these sIL-2R is still unclear. Since high levels of sIL-2R are thought to be an indicator of an activated immune system we investigated the correlation of sIL-2R concentration and prognosis of ovarian cancer patients. sIL-2R measurement was performed on the preoperative sera of 130 patients with benign, and 119 patients with malignant ovarian tumors. The IMMULITE sIL-2R assay by DPC Biermann, Bad Nauheim, Germany was used. In ovarian cancer patients sIL-2R concentrations were significantly higher than in those with benign tumors. By defining the 95th percentile of the sIL-2R concentration distribution in patients with benign diseases as the cut-off (1200 U/ml) 35% of the ovarian cancer patients had elevated concentrations. Concentrations of sIL-2R increased with FIGO stage. FIGO-III patients with highly elevated sIL-2R concentrations tended to have better prognosis than those with sIL-2R levels within normal range in contrast to FIGO IV patients. Since sIL-2R concentrations indicate an immunological activation in ovarian cancer patients our data give hints of the possible role of sIL-2R in the assessment of the risk of recurrence in ovarian cancer patients.

Adenofibroma↗
PubMedDate not supplied

IMMULITE OM-MA assay: a useful diagnostic tool in patients with benign and malignant ovarian tumors.

Measurement of CA125 is well established in the diagnosis and follow-up of ovarian cancer. CA125 determination is usually performed with the monoclonal antibodies OC125 and M11. For the IMMULITE OM-MA chemoluminsence assay (DPC Biermann, Bad Nauheim, Germany) the monoclonal antibody OV185 is used. We analysed CA125 serum concentrations of patients with benign and malignant ovarian tumors with the IMMULITE OM-MA assay by defining a cut-off at 90% specificity level. Sera of 130 patients with benign and 119 patients with malignant ovarian tumors at different FIGO stages were analysed. By calculating a cut-off of 46 U/ml, in 87 ovarian cancer patients, elevated CA125 concentrations were detected (78% sensitivity). There were no significant differences between CA125 concentrations of tumors of different histological type. The measurement of CA125 by IMMULITE OM-MA is a simple and sensitive procedure.

Adult↗
PubMedDate not supplied

A study of age-related variables among physically abused children.

One of every five substantiated cases of child abuse and over 50 percent of child-abuse-related deaths are the result of physical injury. The charts of 266 children, newborn to seventeen years of age, seen at Texas Children's Hospital during a two-year period were reviewed. Each child was assigned to one of four age-groups. Approximately 90 percent of all cases involved children five years old or younger, with 60 percent being less than two years of age. The results suggest that age is an important factor in the location, type, and severity of injuries due to the physical abuse of a child.

Adolescent↗