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Biomedical subjects

M Richardson

Publications and source records attributed to M Richardson.

At least 199 records · Page 11Linked to original sources
PubMed1994

Connected to the future. The latest computer technology can ease paperwork burdens and expand your horizons.

The art of medicine and the science of computing have become irrevocably intertwined in the past few years, as advances in technology have made completing the most tedious and time-consuming tasks a snap. Current technology gives a physician the ability to gather and exchange information at the touch of a button from an ever-growing network of resources around the world.

Forecasting↗
PubMed1994

Physician, manage thyself. Texas doctors form IPAs to gain control of health-care delivery.

Mention managed care to most physicians, and the first thing they think of is loss of independence. Specifically, they associate managed care with having to answer to someone they may not agree with over how to treat their patients and run their practices. As managed care plans penetrate ever deeper into Texas, more and more physicians are looking for ways to minimize that loss of autonomy. And in many cases, they are finding it in the form of physician-driven independent practice associations (IPAs). Such associations are growing rapidly in virtually every corner of the state. Texas physicians, fearful of what health-system reform may bring to them and their patients and determined to control their fates, are becoming allies in IPAs. Texas, in fact, is quickly catching up with many urban states in the formation of IPAs. However, to meet specific local needs, several groups are introducing some unique forms of practice associations to the mix.

Delivery of Health Care↗
PubMed1993

The complete amino acid sequence of a thrombin-like enzyme/gyroxin analogue from venom of the bushmaster snake (Lachesis muta muta).

The complete amino acid sequence of a thrombin-like enzyme with gyroxin activity isolated from the venom of the bushmaster snake Lachesis muta muta was determined by automated and DABITC/PITC microsequencing of the intact protein; fragments derived from it by separate cleavages with cyanogen bromide, iodosobenzoic acid and hydroxylamine; and peptides resulting from enzymatic digestions with trypsin, pepsin, chymotrypsin, and elastase. The protein, which is composed of 228 residues, contains four putative sites of N-linked glycosylation and exhibits significant sequence similarities with other serine proteases reported from snake venoms.

Amino Acid Sequence↗
PubMed1993

On glucose transport and non-enzymic glycation of proteins in vivo.

Non-enzymic glycation is a chance event which may occur whenever a protein is in solution with a reducing sugar. The product of this reaction is a covalently linked glycated protein. Plasma proteins are commonly targets of glycation, particularly in the hyperglycaemic circulation, and yet little is known of the changes to protein function caused by glycation or the catabolic fate of these glycated proteins in vivo. The following article examines the effect of glycation on the catabolism of albumin, the principal glycated protein in plasma, by comparing the catabolisms of radio-labelled samples of rabbit naturally glycated and unglycated albumins in normal and diabetic rabbits. We reason that the relatively small changes (5-10%) to the half-life (T1/2) of albumin and to its distribution to body compartments caused by glycation in vivo may reflect an evolutionary adaptation of albumin (and of all plasma proteins) to resist glycation and, possibly, an accelerated catabolism. Presumably, any increase in the rate of catabolism of a glycated protein followed by its de novo replacement with native (unglycated) protein would create an extra energy demand of the host. In contrast to the vertebrates, the use of non-reducing disaccharides as transport fuels in the circulation systems of insects and higher plants may be an adaptation to allow transport of relatively high concentrations of sugar without the problems caused by glycation.

Animals↗
PubMed1993

Transient morphological and biochemical alterations of arterial proteoglycan during early wound healing.

Proteoglycan alterations in the carotid arteries of normolipemic rabbits during the first 14 days after injury were evaluated by morphometric analysis of ruthenium red-stained sections using transmission electron microscopy and by incorporation of 35S-sulfate. Two types of de-endothelializing injury were compared, air-drying and balloon catheter. Three days after either injury, a transient increase in concentration of 35S-labeled glycosaminoglycans, changes in distribution of 35S-sulfate among glycosaminoglycans, and a two- to threefold increase in 35S-sulfate content (measured as 35S/micrograms glycosaminoglycan) were observed. Morphometric analysis revealed an alteration in medial proteoglycan distribution and morphology at 3 days after injury, which was more evident after air-drying than balloon injury. In response to either injury, metabolically activated smooth muscle cells were associated with very large proteoglycan granules (diameter: > 60 nm) which possibly contained glycosaminoglycans with longer chains and/or altered charge densities. By 14 days after either injury, the distribution of medial proteoglycan returned to normal and a neointima formed. The neointima was thicker following balloon injury, but irrespective of the nature of the injury proteoglycan concentration was higher in the re-endothelialized than in non-reendothelialized areas. The alterations in extracellular proteoglycan of the intima-medial layers induced by these two forms of injury may influence the pattern of wound healing but are not associated with lipid deposition within the time frame examined.

Air Movements↗
PubMed1993

The resistance of cowpea seeds to bruchid beetles is not related to levels of cysteine proteinase inhibitors.

A cDNA encoding a cysteine proteinase inhibitor was isolated from a cDNA library prepared from developing seeds of an insect-resistant line of cowpea. The sequence of the encoded protein was homologous with those of other plant cysteine endoproteinase inhibitors, and with Type 2 cystatins from animals. Southern blot analyses indicated that small gene families were present in both resistant and susceptible lines of cowpea, while northern blot analyses showed similar levels of expression. It is concluded that the levels of expression of the inhibitor do not account for the differences in insect resistance of the two lines.

Amino Acid Sequence↗