On leadership: lessons learned from geese.
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Humans↗
Biomedical subjects
M Rice
Publications and source records attributed to M Rice.
Mentoring: the gift of giving.
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Education, Nursing, Continuing↗
Emergency nursing at a crossroads. Health care reform, hospital redesign, and other trends figure to transform the specialty. But emergency nurses know how to handle crises. Interview by Marietta Lee.
Health care reform, hospital redesign, and other trends figure to transform the specialty. But emergency nurses know how to handle crises.
Career Choice↗
A type-specific conformational epitope on the nucleocapsid of equid herpesvirus-1 and its use in diagnosis.
A type-specific monoclonal antibody was produced by immunizing mice with purified equid herpesvirus-1 (EHV-1). The EHV-1 specific mAb reacted with all the EHV-1 strains tested so far by indirect ELISA, immunofluorescence, and immunoperoxidase tests. No reactions were detected with the EHV-4, EHV-2, or EHV-3 strains tested. The indirect immunofluorescence and immunoperoxidase tests showed that the nuclei of infected cells were predominantly stained by this mAb. Triton treatment of the virus and immunogold labeling experiments indicated that the nucleocapsid of EHV-1 was the target antigen of the mAb. Preliminary results indicated that this mAb might be a useful tool in detecting specific antibody in horses that have been exposed to EHV-1. In a blocking ELISA, antibodies in sera from hamsters, mice and a foal which had been exposed to EHV-1 were differentiated from those in sera of animals exposed to EHV-4.
Animals↗
Clinical results of cryopreserved valved conduits in the pulmonary ventricle-to-pulmonary artery position.
Aortic valved homograft conduits (AVHC) have become valuable in the pulmonary ventricle (PV)-to-main pulmonary artery (MPA) reconstruction in congenital heart defects. Since 1985, 45 patients, ranging in age from 12 days to 32 years, underwent PV-to-MPA reconstruction utilizing cryopreserved AVHC. Operative deaths included seven patients (16%), six of whom died as a result of the complexity of their underlying heart defects. One late death (2%) occurred as a result of infective endocarditis 48 months after conduit placement. The 38 patients who survived the operation remained in the intensive care unit for a mean of 5.7 +/- 1.0 days (median: 4 days; range: 2 to 37 days). The mean hospital stay was 13.0 +/- 1.8 days (median: 9 days; range: 6 to 63 days). The mean follow-up was 40.0 +/- 3.6 months (median: 40 months; range: 10 months to 7.1 years). Only two patients (5%) required reoperation for conduit stenosis with systolic pressure gradients of 60 to 80 mm Hg at 10 and 14 months, respectively, after operation, and both reoperations were successful. During outpatient visits, 16 patients are totally asymptomatic, and 21 patients have minimal symptoms (New York Heart Association class II). Only 10 patients (26%) require digoxin, and 2 patients (5%) need diuretics as part of their medical regimen. Recent echocardiographic examinations show insignificant pressure gradients in all 37 currently surviving patients. Thus, barring operative mortality, which is almost always associated with the nature of the underlying heart defect, the use of cryopreserved AVHC is a safe and effective alternative for PV-to-MPA reconstruction.
Adolescent↗
Detection of astrovirus in the faeces of cats with diarrhoea.
Examination by electron microscopy of faeces from two separate cases of young cats with diarrhoea revealed the presence of 28 nm viral particles morphologically consistent with an astrovirus. No visible cytopathic effect was observed when the virus was inoculated into a feline kidney cell culture.
Journal Article↗
A prospective study of the psychological adjustment of parents and families of children with cancer.
This study compared the psychological adjustment of parents and families of children with cancer, with the adjustment of parents and families in the community. In the weeks after their children's diagnoses, the mothers of children with cancer reported significantly more anxiety and insomnia, somatic symptoms and social dysfunction than mothers in the community. These problems had declined by the time of a 1 year follow-up assessment. However, at the follow-up assessment the mothers of children with cancer reported significantly more symptoms of depression and somatic symptoms than mothers in the community. A similar pattern of findings was evident among the fathers although the fathers generally reported less distress than was reported by the mothers. The results also suggest that a year after the children's diagnoses, the families in which there was a child with cancer were functioning less effectively than the families in the community.
Adaptation, Psychological↗
A case of Burkitt's lymphoma presenting with digital ischaemia.
Paraneoplastic syndromes are rare in paediatric oncology. This report describes a four-year-old Cantonese child whose sole presenting symptom was acrocyanosis who was found to have Burkitt's lymphoma in association with the presence of positive antinuclear antibodies, antineutrophil cytoplasmic antibodies, antiendothelial antibodies and elevated serum immunoglobulin G concentrations. Digital ischaemia has not been described as a presenting symptom of Burkitt's lymphoma, although this association has been reported in 25 patients with other malignancies.
Antibodies↗
Factors associated with closed head injury in a pediatric population.
The purpose of this study was to identify characteristic factors in children who sustained closed head injury (CHI) between birth and less than 15 years of age. A two-year retrospective audit of charts from a large metropolitan pediatric hospital with a trauma center yielded a sample of 138 charts that met the established criteria for inclusion in the study. Using a descriptive design, data were gathered on age, sex, race, cause of injury, severity of injury, season of accident, time of accident and length of hospital stay. Data analysis revealed that males were 1.5 times more likely to sustain CHI than females. Both sexes showed the highest incidence of CHI during the first year with a second less dramatic peak at around 6 years of age. Transportation-related causes accounted for 57.2% of the injuries with falls accounting for another 22.5%. Of the transportation-related injuries, 27.5% were associated with motor vehicle accidents. The lack of restraint use for subjects in the motor vehicle accident group was associated with 5 deaths as opposed to no deaths in subjects who were restrained. Over 70% of the injuries occurred in the time periods of 2:00-6:00 p.m. (31.9%) and 6:00-10:00 p.m. (40.7%). CHI occurred less frequently in the winter (13.8%) than any other season. Using the Glasgow Coma Scale scores, 56.5% of the injuries were categorized as mild, 17.4% were moderate and 26.1% were severe. There was an 8% mortality rate secondary to injury in the sample.
Accidental Falls↗
Adenovirus precipitating antibodies in the sera of brush-tailed possums in New Zealand.
Sera collected from the Australian brush-tailed possum Trichosurus vulpecula in New Zealand in 1975 and 1989 were tested for the presence of antibodies to adenovirus. Of the 231 sera tested in an agar gel diffusion test, eight (3.5%) had precipitating antibodies to the group specific antigen of mammalian adenoviruses. Available data allowed 99/231 sera to be classified as being obtained from either adult (total 62) or sexually immature (total 37) possums. From the adult animals, 4/62 (6.5%) sera were positive, while no reactive sera were detected among the 37 immature animals. The results provide evidence that natural infection by an adenovirus occurs in possums in New Zealand. Further work needs to be carried out to isolate this adenovirus to enable detailed serological and epidemiological studies to be performed. A species-specific possum adenovirus could have potential in the biological control of this species.
Journal Article↗
Analysis of the herpes simplex virus type 1 promoter controlling the expression of UL38, a true late gene involved in capsid assembly.
The cistrons encoding the herpes simplex virus type 1 (HSV-1) UL37 and UL38 genes are adjacent to one another but are transcribed from opposite strands of the viral DNA. The UL37 gene encodes a 1,123-amino-acid protein of unknown function, while the 465-amino-acid UL38 protein is involved in capsid assembly. Previous work from our laboratory indicated that the transcripts encoding these proteins are expressed with significantly different kinetics in productive infection. In the present communication we confirm the kinetic classes and precisely map the cap sites of the UL37 and UL38 mRNAs. A bifunctional reporter gene vector was used to demonstrate that divergent promoters control the expression of these reporter genes in trans-activation assays. The UL38 promoter is functionally separable from that controlling UL37 in a recombinant virus. We used deletion analysis to demonstrate that as few as 29 bases 5' of the mRNA cap site are adequate for full activity of the UL38 promoter in trans-activation assays. Finally, we analyzed the protein-binding properties of the UL38 promoter; several sites that form complexes containing ICP4, with clear homology to those identified in the HSV-1 gamma 42 promoter, are present. Thus, in general, the properties of this promoter are quite similar to those of other gamma promoters.
Animals↗
A simple guide for design, use, and evaluation of educational materials.
Few health workers in Latin America are trained in how to produce effective and appropriate health education materials. In order to provide an instrument and a strategy to help overcome this problem, the Pan American Health Organization developed an effective methodology to orient the design, use, and evaluation of health education materials. This article describes the development of this methodology and summarizes the basic operating principles and criteria for evaluation. These operating principles include developing educational materials from the community perspective, ensuring that they are an integral part of a health education program, relating them to health services delivery, pretesting the materials, and distributing instructions for use along with these materials. An evaluation scale helps health personnel assess materials that have been designed elsewhere and decide if they would be appropriate for their own target audiences. The scale also promotes self-evaluation by the community in the development of its own materials. This simple and straightforward methodology has been successfully applied in courses and seminars as well as in the design, use, and evaluation of health education materials throughout Latin America.
Evaluation Studies as Topic↗
Overwhelming odds: caregiving and the risk of institutionalization.
In this study we explored whether informally or formally provided long-term care services reduce the risk of institutionalization among the impaired elderly and the nature of the relationship between support and risk. We relied principally on the 1982 National Long-Term Care Survey (NLTCS) with supplementary data from the 1984 NLTCS and the national American Housing Survey. Five specific types of effects were tested: direct effects of formal and informal care on institutional risk; buffering effects of support moderating risk factors; supplementation effects of formal support on the risk-reducing impact of informal care; facilitating effects of the housing environment in enabling support to continue; and intervening effects mediating the direct effects. The effects of each type of support differ substantially. The analyses do not confirm the notion that formal, paid care reduces institutional risk. In fact, the use of paid caregivers is associated with greater risk of institutionalization, other things equal. The results for informal care are more mixed. A general measure that accounts for all types of informal support has no effect on institutional risk in any of the formulations tested. But the marginally significant effects of quality and proximate support in the direct effects model are consistent with the hypothesis that caregiving reduces institutional risk. The findings also indicate that a small number of housing or locational features are facilitators of formal support and particular types of informal support.
Activities of Daily Living↗
Pulmonary malacoplakia and Rhodococcus equi infection in a patient with AIDS.
We describe a case of pulmonary malacoplakia and Rhodococcus equi cavitary pneumonia in a patient with AIDS. The relationship between Rhodococcus equi, a rare bacterial human pathogen, and malacoplakia, an unusual type of chronic granulomatous inflammation, is discussed.
Acquired Immunodeficiency Syndrome↗
SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.
We have reconstituted protein translocation across plasma membrane vesicles of Escherichia coli using purified proOmpA and trigger factor, a 63 kd soluble protein. Treatment of membrane vesicles with urea inactivates them for translocation unless a factor present in cytoplasmic extracts is added during the translocation reaction. Sedimentation analysis showed that the stimulatory activity is of distinctly higher mol. wt than trigger factor. Cytoplasmic extracts from a strain that greatly overproduces the SecA protein are highly enriched in the stimulatory activity for untreated membranes and restore translocation to urea-treated membranes, suggesting that this protein is the stimulatory factor. This assay was used to monitor the isolation of SecA protein from the overproducing strain. The purified protein is soluble, yet binds peripherally to membranes with high affinity and supports translocation. Using pure proOmpA, SecA protein, trigger factor and urea-treated membranes, the protein export process was resolved into binding and translocation steps. We find that proOmpA binds to membrane vesicles with or without SecA protein, but that translocation only occurs when SecA was bound prior to proOmpA.
Amino Acid Isomerases↗
Isolation of a reovirus from a broiler chicken flock with high early mortality.
A reovirus was isolated from a flock of 12,000 broiler chickens which experienced a total mortality of 6.3% up to 35 days. The reovirus produced large syncytia in primary chicken kidney cell cultures with eosinophilic intracytoplasmic inclusion body formation. Infected cells reacted with fluorescein conjugated specific anti-reovirus serum. Negatively strained virions had a double shelled appearance with overall diameter of 65 to 70 nm. The nucleic acid had ten discrete segments with electrophoretic mobilities as would be expected for a reovirus.
Journal Article↗
[Minimal standards in the practice of health education--a pioneer project].
PAHO/WHO sought an institution which could be asked to prepare a definition--in concrete, operational terms--of the basic responsibilities and areas of activity of the health professionals in charge of the educational actions appropriate to the health system. The School of Public Health (Health Education area) of the University of S. Paulo accepted this responsibility for the study of the definition of the health educator's role. This article sets out some of the positive results which have arisen, as a result of that study, over the two last years.
Brazil↗
School performance and psychological adjustment of children treated for leukemia. A long-term follow-up.
This paper describes a long-term follow-up study in which the prevalence of emotional/behavioral problems in a group of 32 children and adolescents treated for leukemia is compared with both their siblings and a matched control group drawn from the general population. The performance of the groups at school and in social activities is also compared. Separate reports on the children were obtained from parents, teachers, and the children themselves using Child Behavior Checklist Lists (CBCLs). In general, there are now fewer differences between the leukemic group and the sibling and control groups than in a previous assessment; however, all of the informants consistently report that children treated for leukemia are still performing significantly worse at school than the matched control group. Possible causes for the continuing poor school performance of the children treated for leukemia are discussed.
Achievement↗