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Biomedical subjects

M Reid

Publications and source records attributed to M Reid.

At least 217 records · Page 12Linked to original sources
PubMed1973

Amino acid absorption by mouse ascites-tumour cells depleted of both endogenous amino acids and adenosine triphosphate.

1. Despite the depletion of both their content of exchangeable endogenous amino acids and reserves of ATP, starved hypo-osmotically shocked preparations of the tumour cells accumulated relatively large amounts of (14)C-labelled 2-aminoisobutyrate, l-alanine, glycine, l-leucine, l-methionine, l-phenylalanine and l-serine, against their respective concentration gradients, by a process apparently driven by the spontaneous flow of Na(+) ions into the cellular phase. Dependent on (a) which compound was used, (b) its concentration and (c) the direction of the Na(+) ion gradient, the peak value of the ratio of the cellular to extracellular amino acid concentration varied from about 0.4 to 7. 2. The extent to which ATP increased the ratio was defined for l-methionine. 3. Chemical analysis of the cellular amino acid content showed that this increased in parallel with the absorption of (14)C. 4. The accumulation of l-methionine and of glycine, against their own concentration gradients, continued in the presence of either 0.3mm-ouabain or 10mug of oligomycin/ml. Thus the sodium pump was probably not involved in the process when ATP was lacking. 5. l-Leucine caused 0.72+/-0.12 (s.e.m.; 6) extra equivalents of Na(+) to enter the shocked starved tumour cells in parallel with the uptake of leucine itself. Only a small loss of K(+) was induced. 6. The influx and efflux of l-methionine in preparations depleted of ATP were both markedly accelerated by the presence of Na(+) ions. 7. The observations provide further examples of the application of the ion-gradient hypothesis, according to which Na(+) ions act as co-substrates of the amino acid pump. The quantitative importance of parallel Na(+)-independent systems was studied with a new mathematical model.

Adenosine Triphosphate↗
PubMedDate not supplied

Opioid and benzodiazepine tolerance and dependence: application of theory to critical care practice.

Critical care clinicians frequently manage patient pain and agitation and promote ventilator stability through use of opioids and benzodiazepines. Often, doses of these drugs must be increased considerably over time as they lose their effectiveness-an indication of drug tolerance. Furthermore, patients can experience negative physiologic responses to withdrawal of these drugs-an indication of drug dependence. Withdrawal symptoms due to abrupt discontinuation of drug therapy can be profound and dangerous. It is important that clinicians understand the mechanisms of drug therapies and their potential negative sequelae. The purpose of this article is to present physiologic theories of opioid and benzodiazepine actions, as well as drug tolerance and dependence, as a basis of knowledge for clinical practice. A clinical scenario of an intensive care unit patient is presented, and a care plan is offered, to provide guidance to practitioners who care for patients experiencing the consequences of long-term opioid and benzodiazepine use.

Analgesics↗