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Biomedical subjects

M Reich

Publications and source records attributed to M Reich.

At least 37 records · Page 2Linked to original sources

Isolation and characterization of antagonists for the biocontrol of the postharvest wound pathogen Botrytis cinerea on strawberry fruits.

Antagonistic bacteria and yeasts were isolated from the epiphytic flora of stored strawberry fruits and evaluated for their ability to protect strawberry fruit wounds after harvest against Botrytis cinerea. Among selected potential antagonists, three strains of Candida reukaufii (5L3, 10CL4, 10L2) and one strain of Candida pulcherima (10L8) still protected fruit wounds when applied at 10(3) CFU/wound, reducing lesion or conidiophore development. In the same conditions, two Enterobacteriaceae (10B1, 5B4) highly reduced pathogen development. Strain 5B4 was still highly inhibitory when inoculated at 10(2) CFU/wound. The six strains applied on fruits did not produce any significant change in color, brightness, and firmness of fruits. The two yeasts, 5L3 and 10L8, and particularly the two bacteria, 5B4 and 10B1, were selected for further studies. The four antagonists effectively colonized fruit wounds and strongly inhibited spore germination of B. cinerea in vitro. The bacterial cells surrounded the germinating spores of B. cinerea and attachment of 5L3 cells on germinating spores were additionally observed. Bacterial antagonists, particularly the strain 5B4, multiplied and rapidly used carbohydrates in strawberry fruit juice despite the low pH (pH 3.5). The efficiency of the bacterial antagonists on fruit wounds was related to their growth and nutritional properties.

Beverages↗

[Recognize the victim in need: the effect of situational cues and priming on identification of need for help].

An experimental study investigated the joint influence of priming and situational cues on the perceived neediness of a target. A scrambled sentence test was used to prime two groups with the concepts of neediness and safety. In a third group a neutral concept was activated. A free description of the target and a subsequent adjective rating were applied as dependent measures. Although results indicated that both factors affected the attribution of neediness on a free description, the effects of priming were only apparent on this measurement and disappeared on the second task.

Adult↗

Severe microcirculatory abnormalities elicited by E. coli hemolysin in the rabbit ileum mucosa.

Decreased capillary flow and heterogeneity of microvascular perfusion are hallmarks of septic circulatory disturbances, and the gastrointestinal mucosa is considered to be particularly prone to such abnormalities. We investigated the impact of Escherichia coli hemolysin (HlyA), a medically relevant pore-forming bacterial toxin, on the mucosal microvasculature in a constant-flow blood-perfused rabbit ileum model. Microsensor techniques were employed to assess spatial distribution of mucosal hemoglobin oxygenation and relative mucosal hemoglobin content, as well as mucosal-arterial PCO(2) gap. Administration of low doses of HlyA (0.005 to 0.1 hemolytic units [HU]/ml) into the mesenteric artery provoked a transient vasoconstrictor response. Whereas physiological mucosal oxygenation is homogeneous, severe heterogeneity in capillary blood flow distribution appeared, paralleled by a marked increase in the mucosal-arterial PCO(2) gap. In addition, HlyA provoked a dose-dependent increase in relative hemoglobin concentration (rel Hb(conc)) values and edema formation, suggesting postcapillary vasoconstriction and capillary leakage. The observed changes occurred while fully maintaining mesenteric oxygen delivery. We conclude that low doses of HlyA may elicit severe mucosal microcirculatory disturbances in the rabbit ileum under maintenance of global hemodynamics, reminiscent of septic perfusion abnormalities. Pore-forming bacterial toxins may thus be considered as contributors to splanchnic mucosal damage under conditions of severe infection and sepsis.

Animals↗

A computer based, temperature controlled bipolar electrocoagulation system: II. Optimal temperature and coagulation time.

OBJECTIVE: A computer based, temperature controlled bipolar coagulation system has been developed. This system was evaluated for its efficiency on electrocoagulation of rabbit uterine horns at a low temperature. STUDY DESIGN: In purpose to find an optimal setting, rabbit uterine horns were coagulated at various temperature and time. They were examined histologically and histochemically up to six weeks after coagulation. RESULTS: Electrocoagulation at 90 degrees C for 30 s induced a total occlusion of the lumen when examined six weeks later. CONCLUSION: This coagulation system with low temperature is sufficient for a total occlusion of uterine lumen in the rabbit.

Animals↗

[Bourneville's tuberous sclerosis and autism].

Tuberous sclerosis is often associated with developmental and behavioural disorders including typical or partial autistic syndrome. However, it may be difficult to recognize tuberous sclerosis behind an infantile autism during the early stages of the disease. Therefore, tuberous sclerosis must be regularly looked for on the basis of its major and minor criteria in any cases of infantile autism. The child psychiatrist is preferentially involved in the management of the various aspects of this association, ie, behavioural or character disorders, difficulties in social relationships and communication, mental retardation, feeding disorders, and psychological consequences for the families. The support provided may be complemented by that offered by the Association for Bourneville's tuberous sclerosis.

Autistic Disorder↗

[Superficial damage to the MemoryLens in implantation].

BACKGROUND: Besides silicone and acrylic lenses thermoplastic lenses, such as the MemoryLens, are being increasingly used. Thermoplastic lenses are implanted through a small incision in a rolled state so that they unfold in the capsular bag. Here we describe a complication that has had appeared in two patients. PATIENTS, METHODS AND STATUS: After phacoemulsification a MemoryLens was implanted in 35 years of 35 patients through an incision in the clear cornea revealing two cases of scratched anterior surfaces after implantation. The scratches were caused by the tip of the forceps through excessive pressure and mechanical force during implantation due to an incision slightly too small. CONCLUSIONS: By a lens diameter of 2.31 mm in a rolled state we do not only recommend a forceps with rounded ends but also an incision width of 3.8 mm in order to perform a safe implantation without damage to the lens surface.

Aged↗

[Discussion group: for caregivers confronted with death in the hospital environment].

Caregivers facing death and supporting dying patients don't have often the possibility to express their suffering inherent in their practice. Repression of affects can be harmful increased by all the "unsaid" and leads to splitting defence mechanisms among them. Caregivers will verbalize this suffering if a specific space and time for talking is provided. Paradoxically the medical team found it unnecessary and equated to a "one more meeting". A talking group is considered as a tool for the caregivers and will adapt itself to the institutional functioning. It has not to be denatured from its real function. It has to avoid pitfalls such as paying back conflicts. This group will survive the institutional assaults if some specific requirements are respected. Thus, the group will achieve, or at least facilitate better relationships between caregivers and patients.

Adaptation, Psychological↗