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M R Patters

Publications and source records attributed to M R Patters.

25 records · Page 2Linked to original sources
PubMed1980

Lymphoproliferative responses to oral bacteria in humans with varying severities of periodontal disease.

We assessed in vitro the lymphocyte blastogenic resp onsess of peripheral blood lymphocytes to antigen extracts of a large battery of oral microorganisms in a population of humans with varying severities of periodontal disease. When the magnitudes and frequencies of statistically positive blastogenic responses to various oral microorganisms were compared, three general patterns emerged. The Actinomyces species proved to be potent stimulators of lymphocyte blastogenesis in most subjects tested, whereas Streptococcus sanguis, Campylobacter, and Eikenella corrodens stimulated few individuals. The response to these organisms correlated poorly with the severity of periodontal disease in the tested patients. However, several gram-negative anaerobic organisms, including Bacteroides asaccharolyticus and Treponema denticola, elicited statistically more frequent positive response in subjects with destructive periodontitis compared with patients with gingivitis. These results, taken together with recent microbiological findings, suggest that the specificity of the lymphocyte blastogenic response to antigens of oral bacteria correlates with the presence of these organisms in the subgingival microflora during various periodontal disease states.

Actinomyces↗
PubMed1977

Lymphoproliferative response during resolution and recurrence of naturally occurring gingivitis.

The lymphocyte blastogenic response in humans to oral bacterial antigens was investigated during resolution and recurrence of naturally occurring gingivitis. The following conclusions were drawn: 1. Actinomyces and Fusobacterium and the host responses they evoke, are associated with gingival inflammation. 2. The magnitude of the blastogenic response to these antigens is directly related to the severity of gingival inflammation. 3. Scaling may cause a transient rise in the blastogenic response. 4. Conservative therapy not only improves the clinical state of gingival health, but also interrupts a possible pathogenic mechanism of periodontal disease.

Actinomyces↗
PubMed1976

Blastogenic response of human lymphocytes to oral bacterial antigens: characterization of bacterial sonicates.

Soluble sonicate supernatant preparations were made from Actinomyces viscosus (ATCC 19246), A. naeslundii (ATCC 12104), two strains of Veillonella alcalescens (strain HV-1 and a human oral isolate), Streptococcus sanguis (ATCC 10556), S. mutans (strain 6715-T2), Bacteroides melaninogenicus (strain K110), and Leptotrichia buccalis (isolated from human dental plaque). These supernatants were characterized with reference to their chemical and antigenic components and their biological activity determined by using in vitro lymphocyte blastogenesis as a measure of the host's cellular immune response. The sonicate supernatant of each bacterium contained protein, neutral sugars, methylpentose, and nucleic acids. Protein was the major component in all except L. buccalis, in which neutral sugars predominated. The antigenic components in each supernatant were detected by using rabbit antisera prepared against the whole bacteria and the sonicate supernatant. The supernatants showed a complex antigenic distribution on immunoelectrophoretic analysis. The supernatants were shown to be antigenic and not mitogenic in nature, since neither cord blood lymphocytes nor all adult lymphocytes were stimulated. The supernatant antigen preparations showed a reproducible, dose-dependent, and kinetic response in vitro, which was similar to that seen with the antigen preparation streptokinase-streptodornase.

Actinomyces↗
PubMed1976

Blastogenic response of human lymphocytes to oral bacterial antigens: comparison of individuals with periodontal disease to normal and edentulous subjects.

Cell-mediated immunity in humans to antigens derived from oral plaque bacteria was investigated by using the lymphocyte blastogenesis assay. Subjects with varying severities of periodontal disease including normal, gingivitis, periodontitis, and edentulous were compared. Mononuclear leukocytes were separated from peripheral blood and cultured with antigens prepared by sonication of Actinomyces viscosus (AV), Actinomyces naeslundii (AN), Veillonella alcalescens (VA), Leptotrichia buccalis (LB), Bacteroides melaninogenicus (BM), and homologous dental plaque (DP). The lymphocyte response of subjects with gingivitis or periodontitis was significantly greater than that of normal subjects to antigens of AV, AN, and DP, but did not differ from the response of edentulous subjects. Periodontitis subjects were significantly more reactive than edentulous and normal subjects in response to VA, LB, and BM. These findings suggest that the tested gram-negative bacteria and the host response they evoke are associated with advanced periodontal destruction.

Actinomyces↗