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Biomedical subjects

M R Neuman

Publications and source records attributed to M R Neuman.

At least 55 records · Page 3Linked to original sources
PubMed1981

Chlamydial hemagglutinin: interaction of ether-ethanol extracted fraction with sensitive erythrocytes.

Chlamydial particles and soluble hemagglutinin were separated by differential centrifugation from the supernatant of L-cells and the allantoic fluid of chick embryo infected with C. psittaci 6BC and C. trachomatis TW-3. Concentrated hemagglutinin was fractionated with ether-ethanol; specimens were compared using sensitive erythrocytes of adult white Leghorn chickens. The ether-ethanol extract had a 40- to 80-fold higher hemagglutinin titer than the crude hemagglutinin or the ether-ethanol insoluble fraction. The extracted hemagglutinin also showed a higher complement-fixing activity than the other two fractions. Extracted hemagglutinin was stable for 3 months at 4 degrees and --70 degrees C when sonicated immediately before hemagglutinin; it agglutinated to a similar titer at 37 degrees, 25 degrees and 4 degrees C, showing the reaction to be temperature independent; it agglutinated to a similar titer within a pH range of 7.0 to 8.0 McIlvaine citrate buffer-saline solution and Dulbecco's phosphate buffer solution without Ca++ and Mg++ at pH 7.0 were both suitable for hemagglutinin-titration. Hemagglutination failed to take place in non-electrolyte solutions.

Allantois↗
PubMed1980

A Palladium-palladium oxide miniature pH electrode.

A wire-form miniature palladium-palladium oxide electrode has been fabricated for pH measurement. The electrode exhibits a super-Nernstian behavior and gives a mean pH response of 71.4 millivolts per [pH] (standard deviation, 5.2 millivolts). Uncorrected zero current potential values can be used to determine the pH value of the medium to within 0.012 pH. The electrode should find applications in biological, medical, and clinical studies.

Buffers↗
PubMed1980

QRS wave detection.

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Electrocardiography↗
PubMed1980

Host influence on the density of chlamydiae in Renografin gradients.

Egg-grown and L cell-grown C. psittaci 6BC strains formed two bands when centrifuged through preformed Renografin gradients. No additional bands were observed by extending the time of centrifugation. Particles present in the bands which were collected from the Renografin gradients were examined by electron microscopy. Purified elementary bodies from both egg- and L cell-grown sources were located in a lower, minor bind; egg-derived elementary bodies showed a higher density (p < 0.01) than elementary bodies derived from L cells. Particles present in the major, upper band of both egg- and L cell-derived strains had similar densities and contained mixed populations of elementary bodies and polymorphic reticulate bodies.

Animals↗
PubMed1980

Infectivity of penicillin-induced Chlamydia psittaci forms for cell cultures from the chorioallantoic membrane of chick embryos.

L-cells were infected at high multiplicity with meningopneumonitis organsims (MP) and incubated in medium with penicillin (200 units/ml) and without the drug. At intervals up to 36 hours cells were removed and L-cell associated chlamydial infectivity was determined for cell cultures from the chorioallantoic membrane (CAM) of chick embryos which were treated before inoculation with Diethylaminoethyl (DEAE)-Dextran. In control L-cells, the infectivity decreased at 8 hours and reached it lowest level 16 hours after infection. In penicillin-treated L-cells in contrast, infectivity remained at low levels from 2 and up to 36 hours after infection. At 16 hours after infection about 90% of both penicillin-treated and nontreated L-cells contained single or multiple Giemsa-stained intracytoplasmic inclusions which in both series contained almost exclusively reticulate bodies (RB) by electron microscopy. RB from 16 hour infected penicillin-treated and non-treated L-cells were partially freed from cell debris and their infectivity (ID50) for CAM-culture cells (TC) was assayed 3 times. Penicillin-induced RB had TCID50 of 10(4.0)/ml compared to control RB of 10(4.5)/ml. The TCID50 of the original MP strain for CAM-culture cells was 10(8.0)/ml. Penicillin-induced RB and control RB from 16 hour L-cell samples were frozen and thawed 3 times and titrated in CAM cells; the TCID50 of both series dropped to 10(1.0)/ml while the TCID50 of the original MP inoculum after freezing and thawing 3 times was 10(7.0)/ml.

Animals↗
PubMed1980

A two-channel radiotelemetry system for clinical fetal monitoring.

A radiotelemetry system for monitoring fetal heart rate and uterine contractions during labor has been developed and evaluated. The two-channel transmitter utilizes a miniature intrauterine pressure sensor which frequency modulates a subcarrier. The fetal electrocardiogram picked up by a scalp electrode is summed with the subcarrier and this direct frequency modulates the RF carrier. The transmitter has been constructed in a miniature package using conventional electronic components. A receiver system which obtains the original signals and feeds them to a conventional fetal monitor has been developed for use with this transmitter. Clinical evaluation has shown the telemetry system to provide efficacious signals.

Electrocardiography↗
PubMed1979

Evaluation of fetal monitoring by telemetry.

A radiotelemetry system for the direct monitoring of fetal heart rate and intrauterine pressure during labor has been evaluated at 5 different institutions. A conventional fetal scalp electrode and a special intrauterine pressure sensor are connected to a radio transmitter placed on the patient's thigh. The receiver can be located up to 50 feet away from the transmitter and is either a self-contained monitor or a unit that converts a conventional fetal monitor to a telemetric one. The telemetry recordings are of similar quality to those obtained from conventional monitors. Telemetry allows for greater patient comfort and mobility as well as greater convenience to the clinical staff. Continuous data can be obtained from patients while they are ambulatory or sitting in a chair as well as while they are in bed.

Electrodes↗
PubMed1978

Continuous cervical dilatation monitoring by ultrasonic methods during labor.

Continuous recording of cervical dilatation during labor has been investigated in 13 pregnancies. The recordings were obtained with an ultrasonic cervimeter that continuously monitors cervical dilatation from the transit time of ultrasound signals between two piezoelectric crystals attached on the uterine cervix. A small spring-loaded clip allowed each crystal to be fixed on the rim of the cervical os. Clinical accuracy was +/- 0.6 cm. When the ultrasound recording of cervical dilatation is compared to the intrauterine pressure curve, it is characterized by a baseline and wave-shape curve of dilatation (DWP). The maximal amplitude component is called cervical maximal plasticity. The onset of the DWP is related to cervical resistivity, and the end of DWP reflects the relaxation time of cervical dilatation. The data show that as dilatation enters the active phase of labor, the plasticity, the resistivity, and the duration of relaxation of the cervix increase. These observations are discussed and related to the structural changes of the cervix during labor.

Cervix Uteri↗
PubMed1975

Monitoring of intrapartum phenomena.

We have discussed in the first part of this paper the place of labor monitoring in the delivery of health care to the pregnant woman and the fetus. We have shown that monitoring is only practical and realistic in centers which are equipped to welcome high risk patients. Adequate personnel and facilities should be available on a 24 hr basis. The collaboration of the pediatrician and of the neonatal nursery is essential to the success of the operation. A great part of successful attempts to decrease perinatal mortality and morbidity has to do with prevention. Our population has to be educated during childhood and adolescence about reproduction. The second part of the paper describes the measurement of intrauterine pressure and fetal heart rate and the value of data interpretation. The advantages and limitations of the techniques are outlined. The problems which the doctors and engineers encounter during these procedures are briefly reviewed. It is concluded that monitoring of labor is still in an early developmental stage. Man power and technical innovations22 are necessary to assure a reduction of 15 per cent of the total perinatal mortality with the use of this technique.

Catheterization↗
PubMed1975

Fetal monitoring techniques.

It is seen that there are several possible techniques for monitoring the fetus during labor. In addition to those described above, one can take microblood samples from the fetus and analyze for blood gases and pH. The latter is usually the only parameter measured and often is helpful in assessing the fetus. Yet even with spot checks of this chemical determination added to the monitored data, the overall value of fetal monitoring in definitively diagnosing the onset of significant fetal distress must be questioned. Further work and new monitoring techniques and parameters are clearly needed to improve the care of the high risk mother and fetus during labor and delivery.

Computers↗