Pneumonia. 2. Clinical aspects and treatment.
Explore the source record for details and available documents.
Humans↗
Biomedical subjects
M R Hall
Publications and source records attributed to M R Hall.
Pneumonia. 1. General aspects and classification.
Explore the source record for details and available documents.
Classification↗
Ovarian function in the giant tiger prawn (Penaeus monodon) as determined by in vitro bioassay.
Ovary tissue fragments of the giant tiger prawn Penaeus monodon were incubated in vitro with L-methionine[35S] plus L-cysteine[35S] as a metabolic labeling reagent. The labeled cytoplasmic and secreted proteins synthesized in vitro during incubations under various conditions were subjected to SDS polyacrylamide electrophoresis and visualized by autoradiography. Vitellogenin (Vg) was immunologically identified and shown to be actively synthesized and released into the incubation medium. The synthesis and release of Vg into the incubation medium was optimized and shown to be linear over a 16-h period. Comparisons between different ovarian regions and different stages of development revealed that the level of Vg synthesis and accumulation in the incubation media was variable depending on stage of development and region within the ovary. Coincubation of ovarian fragments with sinus gland extracts showed a dose-related inhibition of total protein and Vg synthesis. The in vitro ovarian bioassay is suitable for examining the effect of hormonal inputs of P. monodon.
Animals↗
Diurnal variation and effects of feeding on blood glucose in the giant tiger prawn, Penaeus monodon.
Previous studies on crustacea have demonstrated significant diurnal rhythms in blood glucose. However, glucose concentration in the blood of food-deprived Penaeus monodon, held in indoor or outdoor tanks, did not exhibit a diurnal rhythm under photoperiods of 8 h light and 16 h darkness (8L: 16D) or under a 13.5L: 9.5D photoperiod, with simulated or natural full moon conditions. Prawns held on photoperiods of constant light, 20L : 4D, 16L : 8D, 12L : 12D, 8L : 16D, 4L : 20D, or continuous darkness did not have significantly different mean blood glucose levels. Mean blood glucose levels varied between 0.77 and 1.39 mmol/L, depending on conditions. Pronounced and significant increases in blood glucose levels occurred within 20 min of feeding, with peak levels after 100 min. The rise in blood glucose level observed after feeding was independent of the eyestalks, and hence putative crustacean hyperglycaemic hormone, and was not from endogenous carbohydrate stores. Under appropriately controlled conditions, blood glucose concentrations can be used as an index of nutritional status in penaeid prawns.
Animals↗
Persistence of human cytomegalovirus DNA sequences without cell-virus homology in human placental explants in culture.
Using histopathology, virology, and molecular probing, we investigated the potential of human first-trimester placental tissue to support human cytomegalovirus (HCMV) infection. Histopathologic examination of infected placental explants revealed cellular changes characteristic of HCMV infection. Immunohistochemical staining of infected explants with human convalescent sera demonstrated expression of HCMV antigens within the cytotrophoblast. Dot blot hybridization of DNA extracted from infected placental tissue using cloned Hind III "W" fragment of HCMV genome indicated the presence of HCMV sequences without virus-cell homology in infected explants from one to ten days post-infection. In general, the sequences detected by the viral probes increased in abundance with time as infection continued.
Antigens, Viral↗