Biomedical subjects

M Purdy

Publications and source records attributed to M Purdy.

17 recordsLinked to original sources

PubMed1997

The problem of self-assessment in nurse education.

Nurse education is expected to fulfil two functions: the facilitation of individual personal development and professional selection. The need to produce competent nurses compromises the facilitation of personal development. This is reflected in the minor role assigned to self-assessment in preregistration nurse education. The problematic nature of self-assessment in nurse education is illustrated through a discussion of three issues: self-assessment and power, self-assessment and stereotyping and self-assessment and context.

Clinical Competence↗

PubMed1997

Humanist ideology and nurse education. I. Humanist educational theory.

Nurse education is dominated by the humanist perspective and the educational theory that it generates. Following a brief description of the perspective's phenomenological foundations and definition of humanist ideology, humanist educational theory is illustrated in an outline of the key contributions of John Dewey, Carl Rogers, Malcolm Knowles and Paulo Freire. The article concludes by noting Freire's sociological challenge to the individualism of the humanist perspective. This challenge recognizes the ideological and social control role of education in securing the reproduction of power relations and leads to questioning the function of individualism and the interests that humanist ideology may serve.

Education, Nursing↗

PubMed1997

Humanist ideology and nurse education. 2. Limitations of humanist educational theory in nurse education.

This article questions the viability of humanist educational theory in nurse education and raises the issue of which interests are served by humanist ideology. The limitations of the humanist approach are traced. Self-directed learning is shown to be problematic in nurse education, leading to tensions between independent learning and required course content, and the appropriateness of student-centred learning to the professional education of nurses is queried. The need to produce safe practitioners compromises the humanist model. Lifelong learning, for example, becomes institutionalized, and its self-directed character transformed into a mandatory process of lifelong professional education. The humanist model has become the new orthodoxy in nurse education and operates as a form of social control. Through its individualism the approach supports a competency model, which in turn restricts the potential diversity of 'product'. This individualistic bias denies the social reality of nursing and fails to empower the nurse by emphasizing individual growth at the expense of social learning. The article concludes that humanist ideology serves the needs of a free-market philosophy. If nurse education is to be challenging it must break with individualism and seek to develop a different rationale, that of a collectivist ideology.

Bias↗

PubMed1994

Isolation of CD34-positive hematopoietic progenitor cells.

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Antigens, CD↗

PubMed1994

Nurse education and the impact of professionalisation.

This article asks what kind of educational process nurse education can follow. Educational theory offers two views; the rationalist and the humanist perspectives. Both involve problems for nurse education. Professionalisation is identified as the major force determining the educational process in nurse education. It prevents progressive humanist education and reinforces traditional rationalist education. This process continues with integration into higher education. Professionalisation must be abandoned as an occupational strategy, if an alternative educational process is to be developed in nurse education.

Education, Nursing, Baccalaureate↗

PubMed1994

Hepatitis C and E in non-A non-B fulminant hepatic failure: a polymerase chain reaction and serological study.

A significant proportion of patients with fulminant hepatic failure have clinical, biochemical and histological features suggestive of acute viral hepatitis, without serological evidence of either hepatitis A or B. The contribution of hepatitis C to such cases of non-A non-B fulminant hepatic failure is presently uncertain while hepatitis E is well recognized as a cause of fulminant hepatic failure in endemic areas. Nested polymerase chain reaction for detection of both hepatitis C and E virus as well as two serological assays for anti-hepatitis C virus and anti-hepatitis E virus western blotting (both IgG and IgM) were performed on acute sera of 42 consecutive cases of non A, non B-fulminant hepatic failure and on convalescent sera of 17 of 20 patients who underwent orthotopic liver transplantation. Fresh liver tissue, obtained at the time of transplantation, was also studied by polymerase chain reaction in eight cases. Evidence of an acute hepatitis E virus infection (hepatitis E virus RNA amplified from serum by polymerase chain reaction or serum IgM positive to western blot) was found in eight patients. One patient had anti-HCV at presentation but assays on later sera proved negative. Convalescent sera and sera obtained after orthotopic liver transplantation were all negative to both anti-HCV assay systems, but HCV RNA was not found in either serum or liver tissue in any case.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗

PubMed1994

Transplantation of enriched CD34-positive autologous marrow into breast cancer patients following high-dose chemotherapy: influence of CD34-positive peripheral-blood progenitors and growth factors on engraftment.

PURPOSE: To evaluate the capacity of enriched CD34-positive (CD34+) progenitor cells to reconstitute hematopoiesis in poor-prognosis breast cancer patients following administration of a high-dose alkylating agent chemotherapy regimen. PATIENTS AND METHODS: Forty-four breast cancer patients received high-dose chemotherapy followed by autologous bone marrow support (ABMS) with CD34+ hematopoietic progenitor cells in five sequentially treated cohorts. Following infusion of CD34+ marrow, cohort no. 1 received no growth factor, cohort no. 2 received granulocyte colony-stimulating factor (G-CSF), and cohort no. 3 received granulocyte-macrophage colony-stimulating factor (GM-CSF). Cohort no. 4 received the CD34+ fractions of both marrow and peripheral-blood progenitor cells (PBPCs) plus G-CSF. Cohort no. 5 received only the CD34+ PBPCs plus G-CSF. Immunohistochemical staining for breast cancer was performed on all hematopoietic cell products before and after the positive selection procedure, to assess quantitatively the level of tumor-cell contamination. RESULTS: Cohorts no. 1, 2, 3, 4, and 5 achieved a granulocyte count > or = 500 x 10(9)/L in a median of 23, 10, 16, 11, and 11 days, with a platelet count greater than 20,000 x 10(9)/L documented in a median of 22, 23, 32, 12, and 10 days, respectively. The time to granulocyte reconstitution was significantly shorter for patients who received CD34+ PBPCs alone (cohort no. 5), or in combination with CD34+ marrow (cohort no. 4), when compared with those who received only the CD34+ marrow fraction (P < .01). From 1 to greater than 4 logs of breast cancer cell depletion were documented after CD34-selection, for patients in whom tumor was initially detected. CONCLUSION: CD34+ marrow and/or PBPCs provide reliable and timely hematopoietic reconstitution in breast cancer patients receiving high-dose chemotherapy. Contamination of both marrow and PBPCs with breast cancer cells was reduced using this positive selection technique.

Adult↗

PubMed1994

Comparative assays of megakaryocyte progenitors in CD34+ peripheral blood progenitor cell fractions.

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Antigens, CD↗

PubMed1993

New strategies in marrow purging for breast cancer patients receiving high-dose chemotherapy with autologous bone marrow transplantation.

High-dose chemotherapy and autologous bone marrow transplantation (ABMT) are commonly used to treat selected patients with high-risk breast cancer. A limitation of ABMT is that clonogenic cancer cells could be collected with the bone marrow and produce a relapse of diseases when reinfused into patients. Purging the marrow ex vivo may eliminate the tumor cells, but it can also delay engraftment. We employed two different purging methods whereby breast cancer cells were depleted without delaying engraftment. The addition of WR-2721 (amifostine) to 4-hydroperoxycyclophosphamide (4-HC) reduced the time to engraftment by 10 days compared with marrow purged with 4-HC alone (26 versus 37 days, respectively). The positive selection of CD34+ hematopoietic progenitors produced engraftment within 21 days. The use of granulocyte colony-stimulating factor (G-CSF) accelerated the engraftment time of CD34+ hematopoietic progenitors to 11 days.

Amifostine↗

PubMed1985

Respiratory epithelial cells masquerading as agents of primary amebic meningoencephalitis: distinguishing features.

CSF does not normally contain motile cells within flagella or cilia. When motile cells are seen in the CSF, they may be incorrectly assumed to be amebae. We studied a patient with a traumatic mucocele, in whom motile ciliated respiratory epithelial cells were seen in the CSF and within a frontal lobe brain abscess.

Amebiasis↗

PubMed1985

Molecular epidemiology of two US orbiviruses: bluetongue virus and epizootic hemorrhagic disease virus.

The degree of relatedness between 4 US serotypes (10, 11, 13 and 17) of bluetongue (BT) virus (BTV) were determined by both oligonucleotide fingerprint analyses of double-stranded (ds) RNA as well as tryptic peptide analyses of viral coded polypeptides. Similar studies were undertaken using different alternate isolates of particular serotypes as well as 2 serotypes (1 and 2) of epizootic hemorrhagic disease (EHD) virus (EHDV), a closely related serogroup of orbiviruses. The results indicate that all BT viruses are genetically related to each other and although EHDV-1 and EHDV-2 originated from a common gene pool, the 2 serogroups, EHDV and BTV, are not genetically interactive.

Bluetongue virus↗

PubMed1984

Cloning of the bluetongue virus L3 gene.

The genes of the bluetongue virus (BTV) serotype 17 have been cloned into pBR322 by tailing both strands of the double-stranded RNA with polyadenylic acid, transcribing them with reverse transcriptase with an oligodeoxythymidylic acid primer, hybridizing the cDNA products, and completing them into duplex structures with the Klenow fragment of Escherichia coli DNA polymerase. After cloning the double-stranded cDNA molecules into pBR322, the complete sequence of the cloned L3 gene was determined. The clone is 2,772 nucleotides long (1.78 X 10(6) daltons), excluding the 3' polyadenylic acid sequence, and has an open reading frame which codes for a protein of some 901 amino acids (103,412 daltons). This clone can hybridize L3 RNA segments of three other U.S. BTV serotypes, BTV-10, -11, and -13 in addition to -17 but not the equivalent RNA segment of epizootic hemorrhagic disease virus of deer, an orbivirus related to BTV.

Animals↗

PubMed1976