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Biomedical subjects

M Pitzurra

Publications and source records attributed to M Pitzurra.

At least 37 records · Page 2Linked to original sources
PubMed1980

[Microbiological dosage of the antitumoral drugs (author's transl)].

The AA. give a complete list of now available microbiological methods to titrate chemo-cytostatic drugs in organic fluids. The described techniques are of particular interest for many reasons: they are easy, inexpensive and rapid; they are reproducible, very sensible and specific. The latter quality privileges the microbiological test in comparison with RIA methods. The introduction into clinical practice of chemotherapy routine monitoring will improve the handling of so active drugs as cytostatics and, toward a more rational treatment of the patients and a better definition of individual minimum active doses.

Animals↗
PubMed1979

[Technic for studying antitetanus immunity].

The Authors carried out a research work on problems relating to technical aspects to the tetanus immunity tests. Some interesting conclusions have been reached: - Purified and diluted tetanus toxin is best preserved at - 20 degrees C, in pH 7.4 physiological salt solution added with 1% peptone. - Vaccination in small laboratory animals must be done injecting adsorbed anatoxin by a microsyringe, in order to avoid dilution of the original compound. - Protection test using low, i.v. toxin doses is more reliable than serological tests in assessing the acquired resistance level in immuno-prophylaxed animals. - Passive haemagglutination test is much more sensitive when done with turkey instead of sheep red blood cells.

Animals↗
PubMed1978

The state of antitetanus protection in the commune of Perugia.

A serological survey was carried out on the population in the Perugia district to assess the protection level against tetanus. The antitoxin titre was evaluated by indirect haemagglutination test in 983 sera from healthy subjects of both sexes and all age-groups. Only 285 males and 144 females, 33.47% of the subjects, had been immunized prior to the investigation. The research made it clear that the serological antibody level does not always correspond to the anamnestic data on previous immunization. Therefore, it appears that prophylactic treatment against tetanus should be based on objective data, that is, serum antibody titre, in all subjects at risk. This information, thanks to the indirect haemagglutination test, is not difficult to obtain.

Adult↗
PubMed1978

Haemagglutination antibody titre levels which give certain protection against tetanus toxin.

The lowest haemagglutination titre which gives safe protection against tetanus toxin, in sera of vaccinated subjects of both sexes and of all age groups has been assessed, by comparative haemagglutination and neutralization tests. It was established that sera with 1/32 haemagglutination antibody titre have a toxin neutralizing capability of greater than or equal to 0.1 I.U./ml. Lower haemagglutination titers do not assure protective capacity against tetanus toxin.

Animals↗
PubMed1978

Serum and skin lysozyme activity in non-diabetic and diabetic subjects.

Lysozyme activity was significantly reduced in the skin of patients with clinical diabetes, but not in the skin of other diabetics or in serum of all these patients. Sex and age had no influence on serum or skin lysozyme activity in either nondiabetic or diabetic subjects. The reduction of cutaneous lysozyme activity is suggested as a factor for the seriousness and the relapses of cutaneous infections in subjects with clinical diabetes.

Adult↗
PubMed1978

Serum antibody level in rabbits submitted to antitetanus active-passive immunization with heterologous horse serum.

Serum antibody titres have been assessed in rabbits submitted to active and active-passive immunization against tetanus with AlPO4 adsorbed vaccine and horse heterologous immune serum. Heterologous antibodies survive in the blood stream no more than six days. Endogenous antibodies, with the adopted vaccination schedule, emerge fifteen days after the first vaccine inoculation. In rabbits undergoing active-passive immunization, the appearance time of endogenous antibodies is delayed, all the more so as the antiserum injected dose increases. Such data stress the opportunity to give up with the use of heterologous immune sera in tetanus prophylaxis, in association with vaccine as well.

Animals↗
PubMed1977

Enhanced early response to tetanus toxoid, in rabbits.

AIPO4 adsorbed tetanus toxoid injected in rabbits according different vaccination schedules, gives rise to antibody responses with varying lag periods. Shortening the intervals among antigen injections, or increasing the quantity of toxoid per dose, makes it possible to reach protective serum antibody levels in 10 days. That is to say, in a period of time shorter than the mean tetanus incubation in man. Although difficult to transfer to man, the exposed data suggest a possible differentiated antitetanus vaccination in subjects at risk, non protected.

Animals↗
PubMed1976

Anti-candida antibodies in human serum. Detection and definition of Ig classes by a new elution technique.

The Partigen Elution Test (P.E.T.), a partially quantitative method for the determination of Ig classes to which the antibodies against superficial Candida antigens belong, has been assessed. The sensitivity of the test is comparable to that of the Ouchterlony test. The P.E.T. has the advantage of simplicity, great reproducibility and absolute specificity. With P.E.T. the level of single class antibodies can be quantitated on the basis of the diameter of immunoprecipitation ring. It seems possible to establish a correlation between the number of precipitation lines in the Ouchterlony test and the diameter of the precipitation ring in LC-Partigen plates. In addition as association among the width of the ring diameter, the Ig classes implicated and the severity of Candida affection is apparent.

Antibodies, Fungal↗