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Biomedical subjects

M Pettersson

Publications and source records attributed to M Pettersson.

63 records · Page 4Linked to original sources
PubMed1982

DNA sequence analysis of the region encoding the terminal protein and the hypothetical N-gene product of adenovirus type 2.

We have determined the complete nucleotide sequence of a 5783-base pair segment of adenovirus type 2 DNA, located between map coordinates 15.8 and 31.7. This region of the adenovirus-2 genome encodes the three segments of the tripartite leader, the i-leader and the two species of virus-associated RNA. The established sequence reveals two translational reading frames encoded by the viral 1-strand which have remarkably large coding capacities. One of these is located between map coordinates 28.9 and 23.5 and encodes a hypothetical polypeptide with an estimated Mr = 74,000. This polypeptide is in an accompanying paper (Smart, J. E., and Stillman, B. W. (1982) J. Biol. Chem. 257, 13499-13506) identified as the precursor for the terminal protein which is covalently attached to the ends of adenovirus DNA. The second large open translational reading frame starts with an ATG triplet at coordinate 22.9 and encodes a hypothetical polypeptide with a predicted Mr = 120,400. This polypeptide is most likely the product of the N-gene, previously mapped between coordinates 18.0 and 22.5 (Galos, R. S., Williams, J., Binger, M. H., and Flint, S. J. (1979) Cell 17, 945-956). The sequence of the r-strand discloses several open translational reading frames, one of which is located within the i-leader.

Adenoviruses, Human↗
PubMed1975

Renin-angiotensin system in essential hypertension.

In a random sample of normotensive and hypertensive fifty-year-old men plasma-renin-activity (P.R.A.), plasma-renin-concentration (P.R.C.), and renin substrate were measured using radioimmunoassay for angiotensin I. P.R.A. in normotensives and untreated hypertensives were normally distributed with slight skewness to the right. The mean P.R.A. for untreated hypertensives (0.65 ng. per ml. per hour) was slightly, but not significantly, lower than that of the normotensive reference group (0.78 ng. per ml. per hour). Previously untreated hypertensives who had been off treatment for four weeks had either high or low P.R.A. depending on the previous treatment. No differences in the angiotensin-generation rate were noted as judged from the P.R.A./P.R.C. ratio. No differences in the renin-substrate concentration between the groups were found. The findings suggest that renin changes in essential hypertenion are secondary to pressure changes. Thus, the renin-angiotensin system may not be of primary pathogenetic importance in the development of essential hypertension.

Angiotensin II↗
PubMedDate not supplied

Gastrin releasing peptide (GRP): effects on basal and stimulated insulin and glucagon secretion in the mouse.

GRP is a pancreatic neuropeptide and may be of importance for the neural control of insulin and glucagon secretion. In this study, we investigated the effects of GRP on basal and stimulated insulin and glucagon secretion in the mouse. Intravenous injections of GRP at dose levels exceeding 2.12 nmol/kg were found to rapidly increase basal plasma levels of both insulin and glucagon. Furthermore, at a low dose level without effect on basal plasma insulin levels, GRP was found to potentiate the insulin response to both glucose (by 40%; p less than 0.05) and to the cholinergic agonist carbachol (by 57%; p less than 0.01). Also, GRP was at this dose level found to potentiate the glucagon response to carbachol (p less than 0.01). Glucose abolished GRP-induced glucagon secretion. Moreover, methylatropine given at a dose level that totally abolishes carbachol-induced insulin secretion inhibited GRP-induced insulin secretion by 39% (p less than 0.05) and GRP-induced glucagon secretion by 25% (p less than 0.01). L-Propranolol at a dose level that totally abolishes beta-adrenergically-induced insulin secretion inhibited GRP-induced insulin secretion by 52% (p less than 0.01) and GRP-induced glucagon secretion by 15% (p less than 0.05). In summary, we have shown that GRP stimulates basal and potentiates stimulated insulin and glucagon secretion in mice, and that the stimulatory effects of GRP on insulin and glucagon secretion are partially inhibited by muscarinic blockade by methylatropine or by beta-adrenoceptor blockade by propranolol. We conclude that GRP activates potently both insulin and glucagon secretion in the mouse by mechanisms that are partially related to the muscarinic and the beta-adrenergic receptors.

Animals↗
PubMedDate not supplied

The use of low-resolution FT-IR spectrometry for the analysis of alcohols in breath.

Fast and reliable diagnostic methods are needed for detection or exclusion of industrial solvents as a cause of intoxication. Analyzing human breath reveals the presence of any volatile substance. A portable Fourier transform infrared (FT-IR) multicomponent point-of-care analyzer was developed for exhaled breath. The analyzer proved to be accurate and precise in laboratory tests for simultaneous measurement of methanol and ethanol in water. Ethanol, in addition to normal contents of breath, was simultaneously analyzed in human experiments, and the results correlated well with blood samples. FT-IR method has a traceable calibration to physical properties of the analyte. The measured spectra can also be saved and analyzed later. Breath analysis with FT-IR is fast and easy, and no preparation of the sample is needed.

Adult↗
PubMedDate not supplied

FT-IR breath test in the diagnosis and control of treatment of methanol intoxications.

A portable Fourier transform infrared (FT-IR) multicomponent point-of-care analyzer was tested for the diagnosis of methanol intoxications. Breath analysis with FT-IR was fast and easy, and no sample preparation was needed. The analyzer was adequately sensitive and accurate in detecting and quantitating clinically relevant amounts of ethanol and methanol in the breath of seriously ill patients. FT-IR spectrometry was also suitable for nearly on-line monitoring of the exhaled ethanol and methanol during hemodialysis. The breath analysis results correlated well with blood samples. The FT-IR method used also has a traceable calibration to physical properties of the analyte, and the measured spectra can be saved for later analysis.

Adult↗