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Biomedical subjects

M Petrov

Publications and source records attributed to M Petrov.

At least 37 records · Page 2Linked to original sources
PubMed1985

[Simplified method for the nonsurgical transplantation of embryos in mice].

A new, simplified method for the nonsurgical transplantation of mouse embryos was developed. Use was made of embryos of sexually mature mice of the Balb/c and C 57BL lines, transplanted intraline or cross-like to recipients of both lines. It was established that nonsurgical methods of transplantation with laboratory animals were less time-consuming and were more readily applicable. Besides, they proved cheaper and did not require special preparatory work as they were simplified as compared with the methods known and used up to then. The new method is a valuable experimental model and contributes to the expansion of fundamental research in biology.

Animals↗
PubMed1983

[Hygienic microbiological studies of a dried egg mixture].

A study on the production of powdered egg melange has been carried out according to the modern principles and the methodology of the hygiene-and-microbiological standardization of food products. It has been established that by microbiologic indices the produce of powdered eggs corresponds to the normative documents, however, some key technologic and hygiene factors are disclosed that affect its microbial flora. Data makes it reasonable to actualize the microbiologic standards provided for in the standardization documents, introduce new microbiologic control indices, and optimize the technologic process and the hygiene regime of producing and storing the product.

Animals↗
PubMed1983

[Experiments with embryo transplantation in cattle in Bulgaria].

Summarized are the results of the first attempts to transplant embryos in cattle in this country over the 1979-1982 period. An average of 6.4 ovulations have been obtained by the induction of superovulation in 48 donor heifers with the use of gonadotropic preparations (Gestyl, Anteron, Folligon) in the middle of the luteal phase at dose of 2000-2500 IU as well as with prostaglandine preparations (Estrumate, Oestrophan) injected 48 hours later. Surgical and nonsurgical methods were employed to recover a total of 78 ova (25 per cent of those that ovulated). Sixty-one (78 per cent) of them were fertilized. Forty-seven (77 per cent) of these were classified as suitable for transplantation. The surgical transplantation of 46 embryos at the age of 7 days (either morula or blastocyst) in 30 synchronized recipient heifers resulted in 9 cases of pregnancy. Three calves were live born.

Animals↗
PubMed1983

[Possibilities of inducing superovulation and obtaining zygotes for transplantation in sheep].

The possibilities were explored of inducing superovulation and obtaining of zygotes from ewes of the Romanov and Tsigai breeds. The pattern used of inducing superovulation included stimulation with the serum gonadotropin preparation Gravohormone applied at the rates of 750 and 1000 IU, estrus synchronization with Estrumate (ICI, England), and ovulation with the use of the choriogonadotropin preparation Gonabion (Jenapharm, DDR). With Romanov sheep both doses of Gravohormone did not affect essentially the ovarian activity--3.4 and 3.7 ovulations were induced, whereupon a total of 5 (20%) and 10 (38%) ova were obtained from all groups of 7 animals each, 3, resp., 2 only of the zygotes being considered of high quality. With Tsigai sheep a differential ovarian response was induced--3.4 and 6.6 ovulations were produced, whereupon 5 (29%) and 15 (35%) ova were obtained, 2, resp., 7 of them being fertilized, but only 2 of the zygotes being considered fit for transplantation from the group treated with 750 IU of the preparation. No substantial differences were established in the blood plasma estradiol-17 beta and progesterone content and dynamics with the two groups of Romanov sheep treated for superovulation. Results showed that the low response of Romanov sheep, the insufficient fertilization of ova, and the low percent of zygotes capable for transplantation should be further discussed.

Animals↗
PubMed1982

[Clinical aspects and pathomorphology of Corynebacterium infection in cattle].

Clinical, paraclinical, and morphological investigations were carried out with a total of 12 cattle (9 calves and 3 cows) with an induced suppurative surgical infection caused by two strains of Corynebacterium pyogenes. It was established that such an experimental infection in cattle clinically ran a chronic course, involving predominantly the urinary and the digestive system as well as the joints. Morphologically, the infection was manifested as a chronic septicopyemia, with purulent nephritis and pyelonephritis, cystitis, splenitis, hepatitis, valvular meningoencephalitis, and absceses in various tissues and organs.

Animals↗
PubMed1982

[Manifestations, treatment and prognosis in acute leukemia].

One hundred fifty one patients were studied--with acute lymphoblast leukosis (ALL) and acute non-lymphoblast (myelogenic) leukosis (ANLL). The disease had a fulminant course in 118 of the patients studied (21.8%). In 73 patients, out of 118 studied, no treatment effect was obtained (61.9%) and the rest 45 patients developed a complete remission (38.1%). In 10 of the patients with no treatment effect, a partial remission was obtained (8.4%). The remission is more frequent in case of ALL. The younger age, at present, remains the most essential prognostic factor. Partial initial leukose infiltration of the bone marrow was found in some of the patients. The bone marrow aplasia is not an obligatory phase of remission. The latter could develop by a gradual restoration of the normal hematopoiesis. The existence of stages with inducing of leukosis processes is possible that follow the reverse way in remission development. Hemorrhages are the most frequent complications in forms with fulminant course, and the infections--in the rest of the patients.

Adolescent↗
PubMed1980

[Economic effect on the milk yield in cows after trimming and treating their hooves].

Experiments were carried out in production conditions on 200 highly productive cows during the lactation period for determining the percentage of hoof illnesses during cow-shed raising, as well as the influence of clipping of hooves and their treatment on milk production. In the case of restricted movement and a long period of not clipping the hooves, the so-called 'shed hooves' were proved with 55.5% of the cows, out of which 18% suffered from an illness. The cases without any visible clinical symptoms for illnesses of the hooves were the cause of a progressive reduction in monthly milk production, ranging, on the average, for a single cow from 3.7 up to 5.3 1 a day. Hoof-clipping brought about the increase of the quantity of daily milk production for a single cow on the average, within the limits 1.5-2.1. In the case of hoof illnesses the daily milk production respectively decreases and reaches 5.4 1 for a single cow. The adequate operative and medicinal treatment despite the gravity of the illness, even during the period of treatment, leads to an increase in daily milk production from 1.50 up to 1.64 1 for a single cow.

Animals↗
PubMed1979

[Changes in the ruminal contents in suppurative surgical infection in cattle].

Rumen content (pH, number of infusoria, quantity of volatile fatty acids and ammonia) was studied in 151 cattle (50 healthy animals, 15 with experimental suppurative infection and 86 with spontaneous suppurative infection). In cases of cattle suppurative infection a statistically significant reduction was observed in the four rumen content indices: pH from 7.61 +/- 0.02 fell to 6.90 +/- 0.06; number of infusoria--from 295 820 +/- 2890 per cm3 fell to 75 867 +/- 1260 per cm3; volatile fatty acids from 919.7 +/- 10.08 mg% diminished to 489.48 +/- 18.91, and ammonia from 27.69 +/- 0.51 mg% was reduced to 12.23 +/- 0.73 mg%. In case of recovery a tendency toward normalizing rumen content was observed.

Ammonia↗
PubMed1977

[Laboratory studies of the urine in suppurative surgical infection in cattle].

One hundred and eight cattle (20 controls, 14 with an experimentally induced and 74 with spontaneously occurred suppurative surgery infection) were studied in terms of their general condition and some of the urine indices--pH, specific weight, proteins, urobillinogen, acetone, sugar, blood, urea and sediment. It was established that when the general status deteriorated and death occurred the urine pH value rose (by 1--1.5), and the specific weight was 0.005--0.010 higher, while the level of urina urea dropped by 0.5--0.6 g%. In 89 per cent of the investigated animals there were 0.1 per percent proteins, and the sediment consisted of 20--30 erythrocytes, from sporadic to 10-12 leucocytes, and from single to 5--6 kidney epithelium cells per field. In 38.8 per cent of the cattle affected with suppurative processes there were tripelphosphates in the sediment. Urobillinogen was found in 63.6 per cent of the cases. Suppurative infections caused by Corynebacterium pyogenes are accompanied by greater changes in the sediment. In almost all of the recovered animals the urine indices came back to normal.

Animals↗
PubMed1977

[Clinical and experimental studies of suppurative surgical infection in cattle].

Investigated were a total of 30 cattle 6 of which had experimental surgery infection and 24 showed spontaneously developing suppurative infection. The animals were studied in terms of general status, blood picture, quality of urine and synovia, and rumen content. The dynamis was followed up of the development of sepsis and purulent artritis. It was found that in case of suppurative surgical infections in cattle the general status was invariably affected, the severity of the process depending on its localization and developmental phase. Changes were observed also in the eye fundus and in the urinary and the digestive system. There were anemia and leukopenia which alternated with leukocytosis, and transient changes in the values of the erythrocyte sedimentation rate and the blood electrolytes. The SGOT values almost constantly increased, and the hemoculture was not positive in all cases.

Animals↗
PubMed1976

[Changes in the serum proteins in suppurative surgical infection in cattle].

Studied were the serum proteins of 3 cattle with a spontaneous suppurative surgery infection and 10 cattle with an experimental infection of the same character. It was found that in such cases there is a rise in the total protein, the alpha and gamma globulins, and a drop in the albumin and the beta-globulins. The experimental suppurative infection caused by Corynebacterium pyogenes produces changes of a higher degree in the protein spectrum than the experimental infection caused by Str. pyogenes. In cases in which the general status of the organism is more strongly involved in the infection process the changes in the protein fractions are more strongly expressed.

Alpha-Globulins↗
PubMed1976

[Clinical studies of an experimental suppurative surgical infection in cattle caused by Cornynebacterium pyogenes].

Studied were clinically and paraclinically six cattle manifesting an experimental suppurative surgery infection caused by Cornebacterium pyogenes, strain 1056. It was established that the infection assumes the course of septicaemia with metastases in the viscera, causing severe disturbances in the general condition and leading to death in most cases. Found were also anemia, leukopenia, that alternated with leukocytosis presenting nuclear shift to left, changes in the erythrocyte sedimentation rate in terms of more rapid settling out of r. b. c. The urine presented protein and sediment consisting of 8 to 10 or numerous erythrocytes, 5 to 6 or 10 to 15 leukocytes, single to 8-10 kidney epithelium cells. The rumen content became alkaline, and the infusoria count dropped.

Animals↗
PubMed1975

Studies on delayed hypersensitivity of patients with Hodgkin's disease during remission.

The studies of skin sensitivity, the reactivity to intradermally transferred lymphocytes and lymphocytic transformation in vitro showed that delayed hypersensitivity had been slightly impaired in patients with Hodgkin's disease during long time remission. The regression of the disease favoured the recovery of the lymphocytic functions and the delayed type immune reactions associated with them. These data suggest that the enhancement of the delayed hypersensitivity of the tumour defence respectively could promote the treatment or prevention of the disease.

Cells, Cultured↗