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Biomedical subjects

M Pastore

Publications and source records attributed to M Pastore.

At least 37 records · Page 2Linked to original sources
PubMed1991

Immunodeficiency and plasma zinc levels in children with Down's syndrome: a long-term follow-up of oral zinc supplementation.

To evaluate the possible effect of zinc treatment on immune disorders in children with Down's syndrome (DS), 38 noninstitutionalized DS children were investigated. Twenty-four patients (63.2%) had plasmatic zinc levels lower than 0.70 microgram/dl ("hypozinkemic," LZn) and 14 patients (36.8%) had levels higher than 0.75 microgram/dl ("normozinkemic," NZn). No correlation was found between the zinc deficiency and recurrence and/or intensity of infections. The absolute numbers of peripheral lymphocytes, the percentages of B lymphocytes, total T cells, and serum IgG, IgA, and IgM levels did not differ between the DS children and the controls. Eight (21%) patients had CD4+ T cell counts below the lowest value for the controls. Seventeen (44%) DS patients had increased levels of CD8+ T cells. The mean percentage of Leu 7+ cells in DS subjects (22.8 +/- 12.9%) was significantly higher than that in controls (15.8 +/- 4.8%) (P less than 0.01). Notably, Ig levels and numbers of lymphocytes in each subset did not show any significant difference in NZn and LZn trisomic subjects. On the contrary the peripheral blood mononuclear cells (PBMCs) from LZn DS children showed a significantly lower proliferative response to phytohemagglutinin (PHA) (S.I. = 23.4 +/- 22.4) than that of PBMCs from NZn DS children (S.I. = 46.1 +/- 21.5, P less than 0.01). A significant increase in DNA synthesis was obtained after oral administration of zinc sulfate (20 mg/kg/day, for 2 months). The lymphocyte response to PHA appeared to be normal in all patients up to 6 months after the end of the zinc treatment and it became low in half of the patients 22 months after therapy.

Administration, Oral↗
PubMed1990

Intravenous immunoglobulin in Diamond-Blackfan anaemia.

Since immunological disorders have been demonstrated in patients with Diamond-Blackfan anaemia (DBA), intravenous immunoglobulins (IVIG) were administered to a 14-year-old girl with DBA and congenital malformations, previously treated with corticosteroids and blood transfusions. No therapeutic effect was observed.

Adolescent↗
PubMed1988

[Reactions caused by Hymenoptera: a first aid problem].

The incidence of reactions to insect bites both local (erythema, pemphigus, oedema, itching) and systemic (urticaria, angioedema, anaphylactic shock) in Borgomanero (NO) National Health Clinic 54 was assessed. The high incidence of medical treatments for this pathology encountered reflects the fact that in the summer and in a rural area a great many people are exposed to this type of emergency. The importance of careful diagnosis (anamnesis, skin allergy tests, RAST is emphasised as a means of identifying cases at allergic risk and providing the appropriate immunotherapy.

Animals↗
PubMed1986

[Study of hypophyseal and gonadal hormones and cases of postmenopausal occurrence of bronchial asthma].

A study was conducted into the hormone profile of women with no previous history of allergy who developed bronchial asthma after menopause in order to discover any differences between these and patients of a similar age with allergic bronchial asthma. The serum levels of the following hypophyseal and gonadal hormones were therefore analysed: luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL), 17 oestradiol (17BE2) in both groups of patients and in a control group of healthy postmenopausal volunteers. The results showed lower FSH and LH and higher 17B7(2) levels in the patients with bronchial asthma but no clinical history of allergy than in either the patients with allergic asthma or the healthy volunteers. When the hormone picture in some of the group 1 patients normalised, the clinical symptoms disappeared. One possible explanation is the following: excess oestrogen in the "non-allergy" patients might have caused an imbalance in the PgF/PgE ratio which is thought to contribute to the regulation of bronchomotor tone. The same phenomena might also have accelerated the release of arachidonic acid and its metabolites.

Age Factors↗
PubMedDate not supplied

Alpha-fetoprotein and acute viral hepatitis type B.

Variations in AFP values in 5 subjects with acute viral hepatitis type B have been studied and also compared with other hepatic enzymes, bilirubin, hepatic markers by means of a seriated drawing of blood. The Authors have stressed the importance of AFP monitoring in hepatics, in order to evaluate a postnecrotic regeneration of the hepatic parenchyma.

Adult↗
PubMedDate not supplied

Correlation between RIA-EIA-ELISA methods for alpha-fetoprotein research.

To-day, the possibility of researching alpha-fetoprotein is given not only by the RIA method but also some immunoenzymatic methods. The Authors have compared three types of methods: RIA, EIA and ELISA, and have also studied the correlation coefficient in 30 samples. Method in solid phase (coated-tubes) and mouse monoclonal antibodies are used for the radioimmunologic method. The ELISA method utilizes two monoclonal antibodies: the former is coated to the walls of the test tubes (mouse monoclonal antibodies) and the latter is labelled with bovine alkaline phosphatase. The EIA methods is a solid phase enzyme immunoassay based on the sandwich principle. It consists of beads coated of goat antibodies and goat anti-AFP antibodies conjugated with horseradish peroxidase. The correlation coefficient is: RIA-ELISA = r 0.95; RIA-EIA = r 0.93; EIA-ELISA = r 0.96. The correlation coefficient from both the RIA method (taken as a reference) and the immunoenzymatic methods is good, so that the passage from RIA to enzymatic methods is possible without problems of under or above dosage of alpha-fetoprotein values.

Enzyme-Linked Immunosorbent Assay↗