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Biomedical subjects

M P Slack

Publications and source records attributed to M P Slack.

At least 55 records · Page 3Linked to original sources

The Fluoretec system for rapid diagnosis of bacteroides infections by direct immunofluorescence of clinical specimens.

Fluoretec, a commercial kit for the rapid diagnosis by immunofluorescence of infections caused by Bacteroides spp was compared with a standard culture method. A total of 1010 specimens were tested for the presence of B fragilis group by Fluoretec F and B melaninogenicus-oralis and asaccharolytic groups by Fluoretec M. Fluoretec F was positive in 123/152 specimens culturing B fragilis group strains. Seventeen specimens were positive by Fluoretec F but negative on culture. Fluoretec M was positive in 21 of 22 specimens from which B melaninogenicus was cultured. The Fluoretec system was convenient in use, results being obtained within one hour of receipt of the specimen.

Bacteroides Infections↗

Wheat-germ agglutination of Neisseria gonorrhoeae. A laboratory investigation.

Wheat-germ agglutination (WGA) was used to identify 168 strains of Neisseria gonorrhoeae and 105 strains of other Neisseria species in a routine laboratory. About one-third of the meningococci reacted with the lectin and titres with some organisms varied on repeat testing. The technique is regarded as unreliable for the identification of Neisseria species.

Agglutination↗

A simple and safe volumetric alternative to the method of Miles, Misra and Irwin for counting viable bacteria.

A rapid and simple method for counting viable bacteria is described. The technique involves the use of a semi-automatic micropipette and disposable glass-capillary tubes. Viable counts obtained by this method were not significantly different from those obtained by the Miles, Misra and Irwin method or by a pour-plate method. The micropipette procedure is less hazardous than techniques involving the use of calibrated dropping pipettes.

Bacteriological Techniques↗

Group B streptococci in pharyngeal aspirates at birth and the early detection of neonatal sepsis.

The pharyngeal aspirates collected from 400 babies at the time of delivery were examined for the presence of bacteria, especially group B streptococci. Aspirates from 79 babies were found to contain viable bacteria, including 4 with group B streptococci; one of these 4 babies developed streptococcal meningitis within 24 hours. The group B streptococci were seen on a Gram-stained film of the aspirate, and were detectable by coagglutination and countercurrent immunoelectrophoresis within 4 hours and by culture after 24 hours. Examination of pharyngeal aspirates may be of value as a screening test for neonatal sepsis.

Delivery, Obstetric↗