Biomedical subjects

M P Lefranc

Publications and source records attributed to M P Lefranc.

At least 19 recordsLinked to original sources

PubMed2001

IMGT, the international ImMunoGeneTics database.

IMGT, the international ImMunoGeneTics database, freely available at http://imgt.cines.fr:8104, was created in 1989 at the Université Montpellier II, CNRS, Montpellier, France, and is a high quality integrated information system specialising in immunoglobulins, T cell receptors and major histocompatibility complex molecules of human and other vertebrates. IMGT provides researchers and clinicians with a common access to all nucleotide, protein, genetic and structural immunogenetics data. This information is of high value for medical and veterinary research, biotechnology related to antibody and T cell receptor engineering, genome diversity and evolution studies of the immune response.

Animals↗

PubMed2001

DNA sequence variability of IGHG3 alleles associated to the main G3m haplotypes in human populations.

The present study investigates the molecular basis of the G3m polymorphism expressed by the heavy constant domains of human immunoglobulins gamma 3 chains. By using a new protocol allowing the specific cloning of IGHG3 genes, a total of 51 full-length IGHG3 genomic sequences (about 2 kb) isolated from African, Siberian, West Asian and European population samples were sequenced. IGHG3 sequences were assigned precise G3m haplotypes on the basis of specific associations between G3m allotypes and IGHG3 RFLPs. Specific DNA substitutions involved in the expression of G3m(5), G3m(6), G3m(15), G3m(16), G3m(21), G3m(24) and G3m(28) allotypes were then deduced, elucidating almost completely the determination of the G3m polymorphism at the DNA level. The molecular evolution of G3m haplotypes was investigated by a maximum likelihood phylogeny of IGHG3 sequences. Sequence clusters are shown to be G3m haplotype-specific, corroborating the Gm molecular model deduced from serology, and showing that populations differentiation is much more recent than G3m haplotypes differentiation. The widely distributed G3m(5,10,11,13,14) haplotype is likely to be ancestral to the other G3m haplotypes presently found at high frequencies in different continental areas.

Alleles↗

PubMed2001

The mouse (Mus musculus) T cell receptor delta variable (TRDV), diversity (TRDD) and joining (TRDJ) genes.

'The Mouse (Mus musculus) T Cell Receptor Delta Variable (TRDV), Diversity (TRDD) and Joining (TRDJ) Genes', the 15th report of the 'IMGT Locus in Focus' section, comprises 7 tables entitled: (1) 'Number of mouse (Mus musculus) germline TRDV genes at 14D1-D2 and potential repertoire'; (2) 'Mouse (Mus musculus) germline TRDV genes at 14D1-D2'; (3) 'Mouse (Mus musculus) TRDV allele table'; (4) 'Mouse (Mus musculus) germline TRDD genes and alleles'; (5) 'Mouse (Mus musculus) germline TRDJ genes'; (6) 'Mouse (Mus musculus) TRDJ allele table', and (7) 'Correspondence between the different mouse (Mus musculus) TRDV gene nomenclatures'. These tables are available at the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt.cines. fr:8104) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, Montpellier, France.

Alleles↗

PubMed2001

IGHV3-associated restriction fragment length polymorphisms confer susceptibility to bullous pemphigoid.

OBJECTIVE: To determine whether the immunoglobulin heavy chain genes contribute to the occurrence of bullous pemphigoid (BP), polymorphisms of both the immunoglobulin constant IGHC and variable IGHV groups were studied in 100 Caucasian BP patients and 143 ethnically matched healthy individuals. METHODS: To analyze the restriction fragment length polymorphism (RFLP) of the IGHG constant locus, a genomic immunoglobulin gamma 3 probe which detects polymorphisms in the gamma constant genes was hybridized to BstEII- or BamHI/SacI-digested germline DNA, while IGHV3 subgroup polymorphism was analyzed by hybridizing a cloned VH3 probe to EcoRI-digested DNA. RESULTS: No difference in the frequencies of the genotypes defined by the constant probe was observed between patients and controls. Analysis of the RFLP obtained with the VH3 probe showed that within the range of 4.5 and 1.5 kb, the observed band pattern was composed of 8 monomorphic and 7 polymorphic bands. Among the latter, 4 allowed to define 10 different restriction patterns. One pattern was shown to be significantly less frequent in patients than in controls. CONCLUSION: IGHV3 polymorphism might be a factor conferring susceptibility to BP.

Deoxyribonucleases, Type II Site-Specific↗

PubMed2001

Nomenclature of the human immunoglobulin heavy (IGH) genes.

'Nomenclature of the Human Immunoglobulin Heavy (IGH) Genes', the 16th report of the 'IMGT Locus in Focus' section, provides the first complete list of all the human IGH genes. The total number of human IGH genes per haploid genome is 170-176 (206-212 genes, if the orphons and the processed gene are included), of which 77-84 genes are functional. IMGT/Human Genome Organization (HUGO) gene names and definitions of the human IGH genes on chromosome 14q32.33, processed gene on chromosome 9 and IGH orphons on chromosomes 15 and 16 are provided with the gene functionality and the number of alleles, according to the rules of the IMGT Scientific chart, with the accession numbers of the IMGT reference sequences and with the accession ID of the Genome Database GDB and NCBI LocusLink databases, in which all the IMGT human IGH genes have been entered. The tables are available at the IMGT Marie-Paule page of IMGT, the international ImMunoGeneTics database (http://imgt.cines.fr:8104) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Chromosomes, Human, Pair 14↗

PubMed2001

Nomenclature of the human immunoglobulin kappa (IGK) genes.

'Nomenclature of the Human Immunoglobulin Kappa (IGK) Genes', the 17th report of the 'IMGT Locus in Focus' section, provides the first complete list of all the human IGK genes. In the most frequent haplotypes, the human IGK locus spans 1,820 kb and the IGKV genes are organized in two clusters separated by 800 kb. In those haplotypes where both the proximal and distal IGKV clusters are present, the total number of human IGK genes per haploid genome is 82 (107 genes if the orphons are included) of which 37-41 are functional. If only the proximal IGKV cluster is present, which is found in a rare haplotype, the total number of genes per haploid genome is 46 (71 genes if the orphons are included) of which 23-25 genes are functional. IMGT/HUGO gene names and definitions of the human IGK genes on chromosome 2p11.2, and IGK orphons on chromosomes 1, 2, 15, and 22 are provided with the gene functionality and the number of alleles according to the rules of the IMGT Scientific chart, with the accession numbers of the IMGT reference sequences, and the accession ID of the Genome Database GDB and NCBI LocusLink databases in which all the IMGT human IGK genes have been entered. The tables are available at the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt.cines.fr:8104) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Alleles↗

PubMed2001

Nomenclature of the human immunoglobulin lambda (IGL) genes.

'Nomenclature of the Human Immunoglobulin Lambda (IGL) Genes', the 18th report of the 'IMGT Locus in Focus' section, provides the first complete list of all the human IGL genes. The total number of human IGL genes per haploid genome is 87--96 (93--102 if the orphons are included), of which 37--43 genes are functional. IMGT/Human Genome Organization (HUGO) gene names and definitions of the human IGL genes on chromosome 22q11.2 and IGL orphons on chromosomes 8 and 22 are provided with the gene functionality and the number of alleles, according to the rules of the IMGT Scientific chart, with the accession numbers of the IMGT reference sequences and with the accession ID of the Genome Database GDB and NCBI LocusLink databases, in which all the IMGT human IGL genes have been entered. The tables are available at the IMGT Marie-Paule page of IMGT, the international ImMunoGeneTics database (http://imgt.cines.fr) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Alleles↗

PubMed2001

Nomenclature and overview of the mouse (Mus musculus and Mus sp.) immunoglobulin kappa (IGK) genes.

'Nomenclature and overview of the mouse (Mus musculus and Mus sp.) immunoglobulin kappa (IGK) Genes', the 19th report of the 'IMGT Locus in Focus' section, provides the first complete list of all the mouse (M. musculus) IGK genes. The mouse (M. musculus) locus spans 3,200 kb. The total number of mouse (M. musculus) IGK genes per haploid genome is 164 (174 if the orphons are included). The functional genomic repertoire comprises 93 IGKV belonging to 18 subgroups, 5 IGKJ and 1 IGKC gene. IMGT gene names and definitions of the mouse (M. musculus) IGK genes on chromosome 6 and IGK orphons are provided with the gene functionality and the number of alleles, according to the concepts of IMGT-ONTOLOGY and to rules of the IMGT Scientific chart, with the accession numbers of the IMGT reference sequences. These tables and figures are available at the IMGT Marie-Paule page of IMGT, the international ImMunoGeneTics database (http://imgt.cines.fr) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Alleles↗

PubMed2000

Characterisation and specificity of two single-chain Fv antibodies directed to the protein tyrosine kinase Syk.

In order to obtain single chain Fv fragments (scFv) specific for the protein tyrosine kinase Syk, we screened a human synthetic phage-display library. Two glutathione S-transferase (GST):Syk fusion proteins containing both SH2 domains of Syk were used to perform three rounds of selection of the library. Among the scFv fragments resulting from the third round of selection, the ones specific for the GST portion of the fusion proteins were eliminated by performing enzyme-linked immunosorbent assay tests on GST:Syk versus GST coated plates, and the monoclonal scFv fragments binding only to the GST:Syk coated plates with high affinities were further analysed. We report here the in vitro characterisation of G4G11 and G6G2 anti-Syk scFvs. G4G11 shows the best performance in immunoprecipitation and immunofluorescence experiments, and G6G2 is able to detect Syk in immunoprecipitation, immunofluorescence and on Western blots. Both scFvs are also able to detect the phosphorylated form of Syk, and neither of them binds to Zap-70, the other member of the Syk family of protein tyrosine kinases.

Animals↗

PubMed2000

IMGT, the international ImMunoGeneTics database.

IMGT, the international ImMunoGeneTics database (http://imgt.cines. fr:8104 ), is a high-quality integrated database specialising in Immunoglobulins (Ig), T cell Receptors (TcR) and Major Histocompatibility Complex (MHC) molecules of all vertebrate species, created in 1989 by Marie-Paule Lefranc, Université Montpellier II, CNRS, Montpellier, France (lefranc@ligm.igh.cnrs.fr ). At present, IMGT includes two databases: IMGT/LIGM-DB, a comprehensive database of Ig and TcR from human and other vertebrates, with translation for fully annotated sequences, and IMGT/HLA-DB, a database of the human MHC referred to as HLA (Human Leucocyte Antigens). The IMGT server provides a common access to expertized genomic, proteomic, structural and polymorphic data of Ig and TcR molecules of all vertebrates. By its high quality and its easy data distribution, IMGT has important implications in medical research (repertoire in autoimmune diseases, AIDS, leukemias, lymphomas), therapeutic approaches (antibody engineering), genome diversity and genome evolution studies. IMGT is freely available at http://imgt.cines.fr:8104. The IMGT Index is provided at the IMGT Marie-Paule page (http://imgt.cines.fr:8104/textes/IMGTindex.html).

Amino Acid Sequence↗

PubMed2000

The human T cell receptor beta variable (TRBV) genes.

'Human T Cell Receptor Beta Variable (TRBV) Genes', the seventh report of the 'IMGT Locus on Focus' section, comprises four tables: (1) 'Number of human germline TRBV genes at 7q35 and potential repertoire'; (2) 'Human germline TRBV genes at 7q35'; (3) 'Human TRBV orphons on chromosome 9 (9p21)', and (4) 'Human TRBV allele table'. These tables are available at the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt.cines. fr: 8104) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Alleles↗

PubMed2000

The human T cell receptor alpha variable (TRAV) genes.

'Human T Cell Receptor Alpha Variable (TRAV) Genes', the eighth report of the 'IMGT Locus in Focus' section, comprises four tables: (1) 'Number of human germline TRAV genes at 14q11 and potential repertoire'; (2) 'Human germline TRAV genes at 14q11'; (3) 'Human TRAV allele table', and (4) 'Correspondence between the different human TRAV gene nomenclatures'. These tables are available at the IMGT Marie-Paule page of IMGT, the international ImMunoGeneTics database (http://imgt.cines.fr:8104) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Chromosomes, Human, Pair 14↗

PubMed2000

The human T cell receptor alpha joining (TRAJ) genes.

'Human T cell Receptor Alpha Joining Genes', the 9th report of the 'IMGT Locus in Focus' section, comprises 3 tables: (1) 'Human germline TRAJ genes'; (2) 'Human TRAJ allele table'; and (3) 'Nucleotide and protein displays of the human TRAJ alleles (overview)'. These tables are available on the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt. cines.fr:8104) created in 1989 by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Alleles↗

PubMed2000

The human T cell receptor beta diversity (TRBD) and beta joining (TRBJ) genes.

'Human T cell Receptor Beta Diversity (TRBD) and Beta Joining (TRBJ) Genes', the 10th report of the 'IMGT Locus in Focus' section, comprises 6 tables: (1) 'Human germline TRBD genes'; (2) 'Human TRBD allele table'; (3) 'Nucleotide and protein displays of the human TRBD alleles (overview)'; (4) 'Human germline TRBJ genes'; (5) 'Human TRBJ allele table', and (6) 'Nucleotide and protein displays of the human TRBJ alleles (overview)'. These tables are available on the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt.cines.fr:8104) created in 1989 by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Alleles↗

PubMed2000

The Teleostei immunoglobulin heavy IGH genes.

'Teleostei Immunoglobulin Heavy IGH Genes', the eleventh report of the 'IMGT Locus in Focus' section, comprises four tables: (1) 'Teleostei IGHV genes'; (2) 'Teleostei germline IGHJ genes'; (3) 'Teleostei IGHC genes and alleles'; (4) 'FR-IMGT and CDR-IMGT length of the Teleostei IGHV genes'. These tables are available at the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt.cines.fr: 8104) created in 1989 by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Animals↗

PubMed2000

The telostei immunoglobulin light IGL1 and IGL2 V, J and C genes.

'Teleostei Immunoglobulin Light IGL1 and IGL2 V, J and C Genes', the 12th report of the 'IMGT Locus in Focus' section, comprises 8 tables: (1) 'Teleostei IGL1V genes'; (2) 'Teleostei germline IGL1J genes'; (3) 'Teleostei IGL1C genes and alleles'; (4) 'Teleostei IGL2V genes'; (5) 'Teleostei germline IGL2J genes'; (6) 'Teleostei IGL2C genes and alleles'; (7) 'FR-IMGT and CDR-IMGT length of the Teleostei IGL1V genes', and (8) 'FR-IMGT and CDR-IMGT length of the Teleostei IGL2V genes'. These tables are available on the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt.cines.fr: 8104) created in 1989 by Marie-Paule Lefranc, Université Montpellier II, CNRS, France.

Animals↗

PubMed2000

Protein displays of the human T cell receptor alpha, beta, gamma and delta variable and joining regions.

'Protein Displays of the Human T Cell Receptor Alpha, Beta, Gamma and Delta Variable and Joining Regions', the 13th report of the 'IMGT Locus in Focus' section, comprises 8 figures: (1) 'Protein display of the human TRA V-REGIONs'; (2) 'Protein display of the human TRB V-REGIONs'; (3) 'Protein display of the human TRG V-REGIONs'; (4) 'Protein display of the human TRD V-REGIONs'; (5) 'Protein display of the human TRA J-REGIONs'; (6) 'Protein display of the human TRB J- REGIONs'; (7) 'Protein display of the human TRG J-REGIONs'; (8) 'Protein display of the human TRD J-REGIONs', and 4 tables entitled: (1) 'FR-IMGT and CDR-IMGT length of the human TRAV genes'; (2) 'FR-IMGT and CDR-IMGT length of the human TRBV genes'; (3) 'FR-IMGT and CDR-IMGT length of the human TRGV genes'; (4) 'FR-IMGT and CDR-IMGT length of the human TRDV genes'. These figures and tables are available at the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http:// imgt.cines.fr:8104) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, Montpellier, France.

Amino Acid Sequence↗

PubMed2000

The mouse (Mus musculus) T cell receptor beta variable (TRBV), diversity (TRBD) and joining (TRBJ) genes.

'The Mouse (Mus musculus) T cell Receptor Beta Variable (TRBV), Diversity (TRBD), and Joining (TRBJ) Genes', the 14th report of the 'IMGT Locus in Focus' section, comprises 8 tables entitled: (1) 'Number of mouse (Mus musculus) germline TRBV genes at 6A-C and potential repertoire'; (2) 'Mouse (Mus musculus) germline TRBV genes at 6A-C'; (3) 'Mouse (Mus musculus) TRBV allele table'; (4) 'Mouse (Mus musculus) germline TRBD genes and alleles'; (5) 'Mouse (Mus musculus) germline TRBJ genes'; (6) 'Mouse (Mus musculus) TRBJ allele table'; (7) 'Correspondence between the different mouse (Mus musculus) TRBV gene nomenclatures'; (8) 'Mouse (Mus musculus) TRBV genes and related human TRBV genes'. These tables are available at the IMGT Marie-Paule page from IMGT, the international ImMunoGeneTics database (http://imgt.cines.fr:8104) created by Marie-Paule Lefranc, Université Montpellier II, CNRS, Montpellier, France.

Alleles↗