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Biomedical subjects

M Ota

Publications and source records attributed to M Ota.

At least 379 records · Page 21Linked to original sources

Effect of testosterone on the androgen receptor in the liver cytosol and nuclei of castrated male rats.

The androgen-binding sites in the cytosol and nuclei were decreased 1 week following castration and this reduced level was sustained for 2 weeks. A maximal increase of the androgen-binding sites in the cytosol was induced by a single injection of testosterone, while the sites in the nuclei were increased gradually with the duration of testosterone injection. These findings suggest that testosterone was able to induce the binding sites in the liver cytosol and nuclei which had been reduced after castration.

Animals↗

[Studies on the dynamic durability of dental restorative materials. (Part 2) Properties for the repeated impact compressive load ot composite resins (author's transl)].

Based on the fracture energy form the compression test of composite resins under the repeated impact loads in compression after stored for a period in water at 37 degrees C, the dynamic durability of commercially used composite resins was evaluated. The results obtained were summarized as follows. (1) The fracture energy of composite resins under the repeated impact compressive loads after stored in water at 37 degrees C is clearly lower than the initial fracture energy. Particularly the decrement of the initial fracture energy for Concise, Epolite 100 and Superlux after stored for 30 days in water at 37 degrees C is influenced considerably both by the physicochemical degradation caused by water and the mechanical deterioration caused by the repeated impact compressive loads, but Clearfil is superior to the mechanical deterioration. (2) In each composite resins, there is a close correlation between fracture energy and compressive strain. In composite resins without Superlux, also a correlation is in fracture energy and compressive strength. (3) In clearfil, concise and Epolite 100, the morphology of fracture in compression test of composite resins which are subjected to and are subjected to the repeated impact loads in compression is classified in a mode of shear fracture, but in Superlux, a mode of crack fracture.

Composite Resins↗

An electron microscopical morphometrical study on pinealocytes of pregnant rats.

A morphometrical study was made on the cut-surface of the pinealocytes in electron micrographs of the pineals from pregnant, puerperal and non-pregnant female rats. No significant differences in the cross-sectional area of the pinealocytes and the nuclei or in the nucleo-cytoplasmic ratio were found among the three groups of pregnant, puerperal and non-pregnant rats. The cross-sectional area of the nucleoli was, however, larger in the pinealocytes of the rats in pregnancy and early puerperium. This has been statistically confirmed by the ranking method (the U-test) of Wilcoxon, Mann and Whitney. The enlargement of nucleoli suggests the enhanced function of the pineal in pregnancy and early puerperium.

Aging↗

Inhibition of testicular androgenesis by urinary antigonadotropins and melatonin in rats.

Comparative in vitro and in vivo studies of the effects of urinary gonadotropin-inhibiting substances and melatonin on the androgenesis in rat testicular homogenates were performed. When the urinary extract containing the inhibiting substances or melatonin was added directly to the incubation medium, and was also injected into rats 24 and 48 hrs prior to sacrifice, either one was effective in suppressing the conversion of pregnenolone to testosterone and/or androstenedione in testicular tissues. The urinary extract exerted the inhibitory effect on the conversion of cholesterol to pregnenolone in vitro and in vivo, whereas melatonin did not have this effect in vitro and in vivo. These findings suggest that the antigonadotropic substances are different from melatonin in their action on androgenesis in the rat testes.

Androstenedione↗

Effect of actinomycin D or puromycin on microsomal testosterone hydroxylase activity enhanced by testosterone in female rat liver.

The injection of testosterone propionate for 4 successive days into female rats resulted in an increase of the in vitro conversion of the hydroxylated testosterones from testosterone by the hepatic microsomal fraction, but no change in the content of microsomal cytochrome P-450 occurred. Actinomycin D or puromycin, which was administered for 4 days with injections of testosterone propionate, prevented the enzyme induction.

Animals↗