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Biomedical subjects

M Olson

Publications and source records attributed to M Olson.

At least 73 records · Page 4Linked to original sources
PubMed1990

Variations of broncho-alveolar lavage techniques affect cell recoveries.

Broncho-alveolar lavage (BAL) has become a routine method of sampling airway and alveolar milieu. Variations of BAL techniques, such as application of chest massage, may have an effect on cell yields. We compared numbers and composition of cells obtained by BAL with and without chest massage from naive rats and from rats infected 7 days earlier with 3,000 larvae of Nippostrongylus brasiliensis. Both techniques revealed significant increases in numbers of agranulocytes and granulocytes after N. brasiliensis infection, but the total yield of cells was 4-5 times greater when massage was used. The use of massage consistently increased the yield of macrophages, but did not result in consistently greater yields of lymphocytes or granulocytes.

Animals↗
PubMed1989

In-service education for home health aides by home health aides.

Gerontological nursing as an emerging specialty is now recognized by all professional nurses as a legitimate professional entity capable of governing the care of older adults through implementation of standards of care. The historical upgrading of care is now reaching its culmination in the newly revised document titled Scope and Standards of Gerontological Nursing as published in 1988 by the Council of Gerontological Nursing of the American Nurses Association. The utilization of these standards should continue to assist nurses in whatever facility they may practice to upgrade the care delivered through partnership with the older adult and their family to a high degree of satisfaction for the consumer and the gerontological nurse practitioner. Expanded knowledge, expertise in skill and care measured by the standards of care are key components in achieving success as a professional nurse and bring pride to the profession of nursing.

Aged↗
PubMed1988

Hyperphosphorylation of N-60, a protein structurally and immunologically related to nucleolin after tumour-promoter treatment.

Okadaic acid, a non-TPA-type tumour promoter, induces hyperphosphorylation of a 60-kd protein in primary human fibroblasts. Treatment with TPA-type tumour promoters (e.g. TPA and teleocidin) did not cause this hyperphosphorylation. Phosphorylation of this protein was not seen at times earlier than 90 min after the addition of 75 ng/ml okadaic acid to the proliferating cell cultures. The presence of inhibitors such as actinomycin D and cycloheximide, did not significantly influence the level of hyperphosphorylation induced by okadaic acid treatment. By immunoblotting using an antibody anti-nucleolin, the 60-kd protein was identified as a fragment of nucleolar protein, nucleolin. Similarly, antibodies against the 60-kd protein cross-reacted with nucleolin. Furthermore peptide mapping, using staphylococcal V8 protease, showed that the 60-kd protein phosphorylated by casein kinase II in vitro and the okadaic-acid-induced hyperphosphorylated 60-kd protein exhibited identical phosphopeptide maps, indicating that there is also structural relatedness between N-60 and nucleolin. Hyperphosphorylation of the nucleolin fragment (N-60) was suppressed by anti-tumour promoter retinoic acid.

Carcinogens↗
PubMed1988

Effects of thyroidectomy, parathyroidectomy and lithium on circadian wheelrunning in rats.

Circadian rhythms and levels of wheelrunning were studied in thyroidectomized, parathyroidectomized, thyro-parathyroidectomized, and sham-operated male rats. Animals were entrained to a 12:12 light:dark schedule, then exposed to constant dim red illumination, and then given a diet containing lithium. Under constant conditions, free-running circadian activity rhythms were shorter, and levels of activity were greater, in thyroidectomized and thyroparathyroidectomized animals. Lithium reversed these effects, lengthening free-running circadian periods in all groups, with a greater reduction of activity observed in animals with thyroids removed. Parathyroidectomy had no clear effects. Since lithium slowed circadian rhythms and reduced activity even in the absence of intact thyroid or parathyroid glands, these effects may have been due to the action of lithium at some other site. The same may be true of other thyroid suppressors reported to affect circadian rhythms. These findings may be relevant to the biological substrates of major affective disorders in humans, which have been associated with abnormalities of thyroid function, abnormally short circadian rhythms, abnormal activity levels, and responsiveness to lithium therapy.

Animals↗
PubMed1987

An ecological study of infected urinary stone genesis in an animal model.

Direct molecular and morphological techniques of modern microbiology were used to monitor the sequential development of bacterial microcolonies and biofilms in a rat model of urinary infection and to demonstrate that the urease activity of the infecting organisms sets in course a series of reactions in which struvite and apatite crystals develop within the matrix of the enlarging bacterial aggregate. This forms multiple stone nidi on the uroepithelial surface upon which succeeding bacterial biofilms develop and with the incorporation of other urine components, such as urinary mucroproteins, establishes a matrix skeleton that becomes mineralised, thus allowing for the growth of the stone in concentric layers. To arrive at this hypothesis, we studied infection stone genesis using a newly developed model for infection-induced bladder stone formation in the rat. We examined in detail the sequential events in the evolving microbial ecology of progressive struvite calculogenesis, using conventional microbiological techniques, direct ultrastructural observation, newly developed ultrastructural cytochemical localisation techniques and immunological procedures for stabilisation of the biofilm glycocalix and stone matrix in the rat model. It was concluded that the organic glycocalix material secreted by the associated bacteria comprises a substantial and aetiologically important part of the infection stone calculogenesis and matrix production.

Animals↗
PubMed1987

Bacterial colonization of intestinal urinary conduit diversion: a morphologic and bacteriologic experimental study.

Cutaneous diversion of the urine through intestinal conduits results in bacteriuria with uropathogenic organisms in up to 80% of patients, many of whom suffer pyelonephritis. Analysis of the bacteriologic data from daily sampling at multiple sites and scanning and transmission electron microscopic studies of sequential loop and autopsy specimens in rabbits with functioning colonic conduits indicate that the pyelonephritis evolves in sequential stages of microbial colonization. Using these data on the natural progression of bacterial infection, we can rationally test the ability of strains of bacteria indigenous to the conduit of this animal model to prevent uropathogenic colonization of the conduit.

Animals↗