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Biomedical subjects

M Olive

Publications and source records attributed to M Olive.

43 records · Page 3Linked to original sources
PubMed1984

Lipid synthesis in isolated rat hepatocytes: activation by insulin and vanadate and inhibition by ouabain.

Lipid synthesis from [1-14C] acetate was stimulated by insulin in rat hepatocytes. Vanadate also enhanced lipid synthesis, although its effect was about 2-3 times higher than that of insulin, whereas ouabain significantly decreased hepatic lipid synthesis. The stimulatory effects of both insulin and vanadate were not dependent on extracellular Ca2+. Also, this stimulation was not mimicked by either ouabain or ouabain plus vanadate, even though they are expected to induce a non-specific increase of cytoplasmic Ca2+ concentration. These results suggest that insulin and vanadate stimulation of lipid synthesis may not be mediated by changes in Ca2+ fluxes through plasma membrane.

Acetates↗
PubMed1982

Effects of diazo-oxo-norleucine on cell kinetics and odontoblast differentiation in cultured embryonic mouse molars.

Diazo-oxo-norleucine (DON), an analogue of glutamine, prevented odontoblast differentiation in cultured tooth germs. Diazo-oxo-norleucine added after the onset of odontoblast differentiation, did not affect the secretion of predentine or the functional differentiation of ameloblasts. DON decreased explant volume and modified cell kinetics, decreasing mitotic index, labelling index and number of grains per nucleus; the 5 phase of the cell cycle was lengthened. These modifications of cell kinetics should be considered when interpreting the effects of DON on odontoblast differentiation.

Animals↗
PubMed1982

Facts and hypotheses concerning the control of odontoblast differentiation.

Numerous studies using amphibians have demonstrated that preodontoblasts emerging from the dental papilla are derived from cranial neural crest cells [4, 12, 46, 64]. However this has not been established for mammals. The history of odonotogenesis begins during the early stages of cranial-facial development when the maxillary and mandibular processes processes develop. Continuous epithelio-mesenchymal interactions condition the histogenesis and morphogenesis of the teeth [24-26, 43, 44, 49, 51, 58] as well as the terminal differentiation of odontoblasts and ameloblasts [23, 47, 52, 54, 59, 61, 67]. During recent years a considerable amount of experimental data relating to differentiation of odontoblasts has been published. We summarize these data and attempt to integrate them in deductive hypothesis concerning the control of odontoblast differentiation.

Adenylyl Cyclases↗
PubMedDate not supplied

Characterization of a mammalian smooth muscle cell line that has retained transcriptional and posttranscriptional potencies.

Unlike skeletal and cardiac muscle cells that differentiate irreversibly, smooth muscle cells (SMCs) retain a high degree of plasticity. During the so-called phenotypic modulation, SMCs can undergo transition between a contractile phenotype and a highly proliferative synthetic phenotype, as apparent from the extinction of numerous smooth muscle (SM) markers when they are passaged in culture. It would be very useful to have an SMC line that can be indefinitely propagated for the cellular and molecular analysis of the mechanisms that underlie the control of SM differentiation. This report describes an immortalized rabbit aorta SMC-derived cell line (U8A4) that has conserved differentiated properties through multiple subcultures. U8A4 cells can grow in the absence of serum and express the SMC markers studied, including SM alpha-actin, SM calponin, SM22alpha, SM alpha-tropomyosin (alpha-TM), SM myosin heavy chain (SM-MHC), and myocardin. U8A4 cells can activate SMC-restricted promoters like those of SM22alpha, SM calponin, and SM-MHC genes as efficiently as described previously for rat SMC lines (PAC1, A7r5, and A10). These cells can also process exogenous alpha-TM transcripts according to an SM-specific pattern. These results demonstrate that the U8A4 cell line constitutes a good alternative model to existing SMC lines that could facilitate the study of the transcriptional and posttranscriptional regulatory mechanisms underlying SMC differentiation.

Actins↗