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Biomedical subjects

M Okuyama

Publications and source records attributed to M Okuyama.

At least 109 records · Page 6Linked to original sources
PubMed1987

A case of interstitial 1q deletion [46,XY,del(q25q32.1)].

A 10-year-old male with interstitial deletion of chromosome 1[46,XY,del(1)(q25q32.1)] is reported. Clinical features of the patient included dwarfism, severe mental retardation, microcephaly, flat nasal bridge, low-set ears, short neck, brachydactyly, clinodactyly of the 5th fingers and bilateral cryptorchidism. Comparison of phenotypic characteristics of the present case with those of six cases previously described with similar deletions of chromosome 1 permits the delineation of an identifiable syndrome.

Abnormalities, Multiple↗
PubMed1986

Effects of diltiazem hydrochloride in diabetics.

The effects of diltiazem hydrochloride on insulin secretion and glucose tolerance were studied in diabetic subjects treated with dietary management alone for their glycemic control. Sixteen diabetic subjects with heart complaints and/or abnormal electrocardiographic findings which were compatible with diagnosis of ischemic heart disease were given diltiazem hydrochloride 90 mg daily for a mean of 9.3 +/- 1.0 (SE) months and oral 100 g glucose tolerance tests (o-GTT) were performed before and after the administration of diltiazem hydrochloride. As a control group, 14 diabetic patients with roughly the same level of fasting blood glucose and the same degree of glucose intolerance were selected, and in these patients oral 100 g glucose tolerance tests were also performed before and after a mean follow up period of 12.6 +/- 1.5 months. Although improvement in glucose tolerance was observed at the end of the trial in the group of patients with diltiazem hydrochloride, control group also showed improved glucose tolerance and there was no significant difference in the change of glucose tolerance between both groups. No significant change of IRI response in oral 100 g glucose tolerance test was observed in both groups. We could, therefore, conclude from these observations that diltiazem hydrochloride with usual clinical dosage dose not exert unfavourable effects on glucose tolerance and insulin secretion in the mild diabetic patients.

Aged↗
PubMed1984

Two-dimensional gel analysis of a second family of class II molecules by polymorphic HLA-DR4,5, and w9 monoclonal antibodies.

Human Ia-like, class II molecules were isolated by immunoprecipitation with monoclonal antibodies from various HLA-D/DR homozygous cell lines and were analyzed by two-dimensional gel electrophoresis. The monoclonal antibody PLM12 reacted with B cells carrying DR4, DR5, DRw6.2, and DRw9 phenotypes, and its reactivity perfectly correlated with the previously defined TB21 (MB3-like) specificity. Class II molecules detected by PLM12 were structurally distinct from those precipitated by the anti-DR monoclonal antibody NC1 on all HLA-DR4, DR5, DRw6.2, and DRw9 homozygous cell lines and showed polymorphism in heavy and light chains among these cell lines. The monoclonal antibodies PLM2 and PLM9 only reacted with B cells carrying DR5 and DRw6.2 and also detected a distinct set of class II molecules from those precipitated by NC1 but identical to those of PLM12. Thus, PLM2 and PLM9 serologically detected a new subtypic antigen of the PLM12-reactive class II molecules. Furthermore, the antibody NC1 precipitated two light chains and one heavy chain from HLA-DRw6.2 homozygous cell line EBV-Sh. The result indicated the presence of three sets of class II molecules: two in a DR family and another carrying the polymorphic determinants detected by PLM2, PLM9, and PLM12 in a second family.

Animals↗
PubMed1984

Tumor hypoglycemia induced in nude mice by a heterotransplantable human ovarian carcinoma line (OCL-1).

Tumor hypoglycemia induced by a heterotransplantable human ovarian carcinoma line (OCL-1) was described. Plasma glucose decreased to 36 +/- 9 mg/dl (S.D.) at 8 to 12 weeks after the transplantation. Significant amounts of immunoreactive insulin and insulin-like active substance could not be detected in tumor tissues. Plasma immunoreactive insulin levels were low, and glucagon levels were high in OCL-1-bearing nude mice, compared with the control. Light- and electron-microscopically, tumor cells possessed large amounts of glycogen, and this finding was also biochemically confirmed. OCL-1 tumor showed high glycogen synthetase activity compared with other control tumors, while glycogen phosphorylase activity was the same level as other tumors. The high glycogen synthetase activity was considered to be the cause of glycogen accumulation in tumor cells. Hypoglycemia in OCL-1-bearing nude mice was considered to be caused by abnormal redistribution of glycogen, i.e., marked accumulation of glycogen in tumor tissues and depletion of glycogen in the host liver. This OCL-1 tumor-nude mice system was thought to be a good model for research on the mechanisms of tumor hypoglycemia occurring in cancer patients with nonpancreatic islet cell tumors.

Animals↗
PubMed1983

Preparation of membrane fraction from herpes simplex virus-infected cells which induce cytotoxic T lymphocytes.

The immunogenic capacity of herpes simplex virus (HSV)-infected cells and their subcellular membrane fractions was investigated by assessing the anti-HSV cytotoxic T lymphocyte (CTL) response in cultures of spleen lymphocytes from HSV-primed BALB/c mice. Methylchloranthrane-induced fibrosarcoma (Meth A) cells infected with HSV (HSV-Meth A) were fixed either with glutaraldehyde or by heating at 56 C to preserve their immunogenic competence and then used as a stimulator. Microsomes and plasma membranes were prepared from HSV-Meth A and their immunogenic activities were determined. Through the recovery of stimulatory activity in the plasma membrane fraction was half of that in the microsome fraction, the activity in the former was much more stable than in the latter and the plasma membrane fraction proved to be well qualified as an immunogen for anti-HSV CTL induction. Upon purification, the specific activity of the membrane fraction, on the basis of protein concentration, increased 43-fold.

Animals↗