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Biomedical subjects

M Okuda

Publications and source records attributed to M Okuda.

At least 415 records · Page 23Linked to original sources
PubMed1976

Factors influencing nonspecific binding of glucocorticoids in myocardial tissue.

Myocardial slices from the left ventricle of cat hearts were incubated in Krebs-Henseleit buffer containing 10 mM glucose which were gassed with 95% O2 and 5% CO2. Tritiated dexamethasone (DEXA 0.171 MM) or methylprednisolone (MP 0.805 mM) was added under varying conditions of temperature, pH, and in the presence of various metabolic inhibitors. Glucocorticoid uptake by myocardial tissue was found to be temperature-dependent, plateauting after 60 min of incubation at 0.21 mumole of DEXA/g of tissue and 0.95 mumole of MP/g of tissue at 37 degrees. Steroid uptake was retarded about 50% at 0 degrees. Increasing the concentration of either steroid resulted in a linear increase in the uptake of that steroid. Incubation in the presence of metabolic or SH-inhibitors produced no significant changes in glucocorticoid uptake. Optimal glucocorticoid uptake occurred between pH 7.3-7.4. Thus, myocardial cells appear to be able to take up large quantities of glucocorticoids, but the uptake process does not appear to be energy-dependent.

Animals↗
PubMed1975

Response of the human nasal mucous membrane to anti-human IgE serum.

The response of the nasal mucous membrane to anti-human IgE serum was examined. The application of anti-IgE sera to the nasal mucous membrane induced a nasal allergy-like symptom as well as nasal eosinophilia. This symptom was observed in the majority of nasal allergy cases and in some vasomotor rhinitis cases, while not in non-allergic normal persons. The nasal eosinophilia, however, increased not only in allergic patients, but in non-allergic persons in whom no nasal symptoms were induced. Such a nasal reaction was not induced with anti-IgA sera or anti-IgG sera. The character of the reaction was presumed to be specific to anti-IgE and was predominantly a reversed type anaphylaxis in the nasal mucous membrane. The sensitivity of the nasal mucous membrane to anti-IgE decreased in the course of the hyposensitization with house dust. The cause of this phenomenon was obscure.

Administration, Intranasal↗
PubMed1975

Studies on interrelations between eosinophilia, serum IgE and tissue mast cells.

In an examination of a variety of diseases associated with a peripheral blood eosinophilia it is evident that the eosinophilia is not necessarily accompanied by increased amounts of serum IgE. Tissue eosinophilia occurring with mast cell hyperplasia and usually increased amounts of IgE, indicate atopic allergy. It is considered that the eosinophilic granuloma of soft tissue (Kimura's disease) is an atopic disease and quite different from histiocytosis X.

Abscess↗
PubMed1975

IgE and IgE antibody to mite in nasal fluid.

The levels of total IgE and IgE antibodies to mite per unit quantity of nasal fluid were successfully determined by our special method of collecting nasal fluid. The mean value of IgE was 80 +/- 101 U/ml, and that of IgE Ab 1.45 +/- 1.29/ml (RAST score) in NF. Nasal IgE concentration was approximately one twenthieth of serum IgE on the average, and nasal IgE Ab to mite was one half of serum IgE. The IgE Ab/IgE ratio was nine times greater in NF (0.0181) than in serum (0.0022). The concentration of IgE Ab to mite was very well correlated between serum and NF (correlation coefficient of 0.83), while that of IgE was not (coefficient of 0.51). IgE Ab to mite was also well correlated to IgE (coefficient of 0.78) in NF while it was not in serum (coefficient of 0.48). The correlation coefficient of nasal/serum IgA was 0.41; that of IgE/IgA in NF was 0.31; and that of IgE/total protein in NF was also 0.31. The possibility of local production and secretion of IgE Ab to specific allergen is discussed in detail.

Adolescent↗