The mouse protein phosphatase 2Cbeta (Ppm1b) gene maps to the distal part of mouse chromosome 17.
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Animals↗
Biomedical subjects
M Oishi
Publications and source records attributed to M Oishi.
Construction of highly extensive polymorphic DNA libraries by in-gel competitive reassociation procedure.
Differential genomic DNA libraries between two mouse strains and from two human individuals were constructed by means of the in-gel competitive reassociation (IGCR) procedure, a procedure developed for cloning altered anonymous restriction fragments. The libraries were highly enriched in RFLP fragments, approximately 60 and 40% for the mouse and human libraries, respectively, and, more importantly, maintained most of the original complexities of the RFLP fragments. Therefore, differential genomic DNA libraries constructed by the IGCR procedure, particularly for human genomic DNA, should offer highly extensive sources for polymorphic DNA sequences necessary for a variety of genome analyses, including studies on the origin and mechanism of biological diversity among the same species.
Animals↗
Beneficial effects of prostaglandin E1 on hemodynamic changes during liver transplantation in pigs.
The vasodilative action of prostaglandin E1 (PGE1) on the systemic and pulmonary circulation was investigated in swine models of orthotopic liver transplantation. In the PGE1-treated group (n = 8), PGE1 (0.05 microgram/kg per minute) was intravenously infused from the onset of the anhepatic stage to 30 min after revascularization. During the anhepatic stage, PGE1 decreased systemic vascular resistance without a corresponding hypotension, so cardiac output was maintained at a higher level. In the control group (n = 8), pulmonary vascular resistance increased to 3 times the anhepatic value during reperfusion, accompanied by a decline in cardiac output with a 28% decrease in blood pressure. In the PGE1-treated group, on the other hand, pulmonary vascular resistance was maintained within the normal range without any associated decrease in cardiac output. The blood pressure decreased slightly by 12%. In conclusion, in this model, PGE1 increased cardiac output without hypotension during the anhepatic stage and also prevented postreperfusion pulmonary hypertension and the subsequent systemic hypotension.
Alprostadil↗
Central motor conduction time in patients with periventricular lucencies.
Central motor conduction time and regional cerebral blood flow were measured before and 20 min after intravenous injection of 17 mg/kg acetazolamide in 10 patients with periventricular lucencies (PVL) and 10 age-matched healthy controls. Central motor conduction time was measured using a magnetic stimulator and regional cerebral blood flow was measured by stable xenon computed tomography method. The central motor conduction time was significantly longer in the patients with PVL than in the healthy controls and was shortened significantly by the intravenous injection of acetazolamide in the patients with PVL. The blood flow not only in the periventricular white matter but also in the cerebral cortex and the cerebral white matter was significantly lower in the patients with PVL than in the healthy controls. The intravenous injection of acetazolamide increased significantly the regional cerebral blood flow except in the PVL areas. The prolongation of the central motor conduction time may be at least partly related with decreased blood flow in the cerebral cortex and cerebral white matter.
Acetazolamide↗
Histologic investigation of hollow implants retrieved for psychological reasons.
OBJECTIVE: The purpose of this study was to radiologically, histologically, and histometrically evaluate bone in the hollow portion of three implants retrieved for psychological reasons. STUDY DESIGN: Three hollow implants retrieved from two patients were studied. We investigated the radiologic and histologic changes of these implants with the use of radiographs, light microscopy, image processing, and fluorescent microscopy. RESULTS: There were no radiologic and histologic degeneration around the implants. Histometric analysis of the hollow indicated that the average percentage of bone contact rate was 33.5% in case 1, 74.5% in case 2, and 18.4% in case 3; the average percentage of bone filling was 25.1%, 33.9%, and 6.6%, respectively. There was a great variation among the three cases in bone to implant contact and bone filling. CONCLUSION: The hollow portion in case 1 that penetrated into the maxillary sinus was encapsulated with fibrous tissue. The amount of bone tissue in the hollow portion seems to depend on the initial bone quality of the recipient sites.
Adaptation, Psychological↗
Evaluation of fasudil hydrochloride treatment for wandering symptoms in cerebrovascular dementia with 31P-magnetic resonance spectroscopy and Xe-computed tomography.
This is the first report on the evaluation of treatment with fasudil hydrochloride, a novel intracellular calcium antagonist, for wandering symptoms in patients with cerebrovascular dementia by using 31P-magnetic resonance spectroscopy (MRS) and Xe-computed tomography (CT). The subjects studied were two patients with cerebrovascular dementia who had had frequent wandering episodes. The clinical diagnosis was Binswanger-type cerebral infarction in patient 1 and sequelae of cerebral bleeding and multiple lacunar infarctions in patient 2. Treatment with fasudil at 30 or 60 mg/day was given orally for 8 weeks. The wandering symptoms disappeared in both patients during the treatment and reappeared a few days after discontinuation of the treatment. Mental tests indicated that memory was mildly improved during the treatment. Pretreatment 31P-MRS findings revealed decreases in relative signal intensities of phosphomonoester and phosphodiesters and an increase in that of mean adenosine triphosphates. After treatment, these findings disappeared. The regional cerebral blood flow values by Xe-CT in both patients did not show significant changes from before treatment to the values after treatment. These results suggest that the efficacy of fasudil for the wandering symptoms and mental function observed in our patients may have been related to a direct effect on intracellular energy metabolism.
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine↗
Effects of sodium ozagrel on hemostatic markers and cerebral blood flow in lacunar infarction.
The present study was designed to examine the effects of sodium ozagrel on hemostatic markers and cerebral blood flow in lacunar infarction. Ten cases of lacunar infarction in which sodium ozagrel was given (administered group), 10 cases of lacunar infarction in which sodium ozagrel was not given (nonadministered group), and 10 age-matched controls in which cerebrovascular diseases were absent but risk factors were similar to those of the patients (control group) were studied. Intravenous infusion of 80 mg of sodium ozagrel was done twice a day for 2 weeks. Platelet factor 4, beta-thromboglobulin, and fibrinopeptide A were significantly higher in the administered and nonadministered groups than in the control group at the time of admission. Platelet factor 4, beta-thromboglobulin, fibrinopeptide A, and thromboxane B2 were decreased significantly by the administration of sodium ozagrel. The blood flow in the cerebral cortex was significantly lower in the administered and nonadministered groups than in the control group. The blood flows around the infarcted area, in the cerebral cortex, and in the cerebral white matter were significantly increased by the administration of sodium ozagrel. Sodium ozagrel is considered to decrease platelet aggregation and increase cerebral blood flow by decreasing thromboxane A2, which has a platelet-aggregating and a vasoconstricting action. Sodium ozagrel is considered to be effective in the acute phase of lacunar infarction.
Aged↗
Evaluation of the peripheral circulatory status of the neonate during homeothermal adjustment by plethysmo-time-interval.
The plethysmo-time-interval (PTI) is the time interval between the beginning of the QRS complex on an ECG and the upstroke of the pulse wave on a plethysmogram, as measured by pulse oximetry. In the present study, we investigated homeothermal acclimatization to the extra-uterine environment in human neonates using the Coretemp thermometer and a pulse oximeter. Temperature was measured at three sites: the central deep temperature (CDT) on the upper sternum, the peripheral deep temperature (PDT) on the flat part of the left sole of the foot and the surface temperature (ST) at the side of the abdomen. After delivery, CDT and ST were higher than PDT. The difference between CDT and PDT was large at first, but gradually decreased. PDT, initially in the range of 32.4 +/- 0.28 degrees C, reached a stable value (34.4 +/- 0.41 degrees C) at 2.5 h after delivery. PTI was prolonged in parallel with PDT. The difference between CDT and PDT probably reflected the contraction of skin vessels, particularly the arterioles, which occurs as a body defense mechanism against heat loss. As PTI was prolonged in parallel with PDT, we demonstrated objectively that this catch-up phenomenon of PDT after delivery was affected by the increase in skin blood flow as a result of dilatation of peripheral arterioles. It was concluded that PTI can be used to evaluate the peripheral circulatory status of the neonate, even during homeothermal adjustment after birth, by applying a new principle of pulse oximetry that is widely used in neonatal intensive care units.
Acclimatization↗
Bilateral thalamic infarctions can sometimes accompany midbrain infarction and rarely accompany pontine infarction.
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Cerebral Infarction↗
Active leprosy treated effectively with ofloxacin.
The patient is a 25-year-old Filipino who showed polymorphous eruptions over the whole body, right ulnar nerve paresis, polyneuropathy and hypalgesia in the area of eruptions. Because the biopsy specimen showed foam cells, histiocytes, epithelioid cells, many Mycobacterium leprae and no giant cells, the diagnosis of borderline-lepromatous (BL) type was made. The symptoms were improved by the administration of 300 mg/day ofloxacin. Because the monotherapy using ofloxacin has been reported to be effective in all 5 previously reported cases of BL type leprosy, it may be recommended for a larger number of leprosy cases.
Adult↗
Effects of intravenous L-dopa on P300 and regional cerebral blood flow in parkinsonism.
The P300 and regional cerebral blood flow were measured before and after intravenous injection of L-dopa in 10 patients with idiopathic Parkinson's disease and 10 patients with vascular parkinsonism. The P300 was measured with an evoked potential recorder using an oddball paradigm and the regional cerebral blood flow was measured using the stable xenon computed tomography method. The P300 latency was significantly longer and the regional cerebral blood flow in the cerebral cortex and basal ganglia was significantly lower in the Parkinson's disease group and the vascular parkinsonism group than in the age-matched healthy control group. The intravenous injection of L-dopa improved these abnormalities significantly in the Parkinson's disease group but did not improve these abnormalities in the vascular parkinsonism group. Cognitive function is considered to be impaired in Parkinson's disease and vascular parkinsonism and L-dopa is considered to improve it in Parkinson's disease.
Aged↗
Postreperfusion syndrome in swine liver transplantation: comparison between orthotopic liver transplantation and total hepatectomy with portacaval shunt using aortic graft.
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Animals↗
[A case showing recurrence of myelopathy and optic nerve disturbance accompanied by pusturosis palmaris et plantaris and positive anticardiolipin antibody].
The patient is a 62-year-old woman who was admitted to our hospital with chief complaints of paresthesia in the four extremities and the trunk and gait disturbance in February 1993. Habitual abortions and left retrobulbar optic neuritis were present in the past history. She had been suffering from pusturosis palmaris et plantaris for 10 years. On admission, weakness of the distal muscles in the right upper extremity and severe disturbance of the deep sensations in the bilateral lower extremities and the right upper extremity were present. With the progress of the neurological symptoms, pusturosis palmaris et plantaris progressed. After the admission, the neurological symptoms became better by the administration of corticosteroid hormone medicine. After that, myelopathy and left optic nerve disturbance relapsed and IgG anti-cardiolipin antibody in the serum was present. The neurological symptom, pusturosis palmaris et plantaris and positive IgG anti-cardiolipin antibody in the serum may be caused by immunological mechanism. Anti-phospholipid antibodies such as IgG anti-cardiolipin antibody in the serum should be examined in patients with clinical multiple sclerosis like our case.
Abortion, Habitual↗
[Ipsilateral oculomotor nerve palsy and contralateral downbeat nystagmus due to unilateral paramedian thalamopenduncular infarction--a case report].
A case of midbrain and thalamic infarction which showed complete oculomotor nerve palsy of the ipsilateral eye and monocular downbeat nystagmus, ptosis, upward movement disturbance and adduction disturbance of the contralateral eye was reported. The patient is a 53-year-old woman who was admitted to our hospital because of sudden onset of double vision. Head magnetic resonance imaging showed unilateral midbrain and thalamic infarction. The midbrain lesion was located in the paramedian area and the bilateral ptosis, bilateral upward gaze palsy and adduction disturbance of the contralateral eye were considered to be caused by the lesion involving the unilateral oculomotor nucleus. This case is considered to be important because the association of contralateral monocular downbeat nystagmus is very rare.
Cerebral Infarction↗
Preference of the recombination sites involved in the formation of extrachromosomal copies of the human alphoid Sau3A repeat family.
The human alphoid Sau3A repetitive family DNA is one of the DNA species that are actively amplified to form extrachromosomal circular DNA in several cell lines. The circularization takes place between two of the five approximately 170 bp subunits with an average of 73.1% homology as well as between identical subunits. To investigate the nature of the recombination reaction, we cloned and analyzed the subunits containing recombination junctions. Analysis of a total of 68 junctions revealed that recombination had occurred preferentially at four positions 10-25 (A), 40-50 (B), 85-90 (C) and 135-160 (D) in the 170bp subunit structure. Two regions (B and C) were overlapped with the regions with higher homology between subunits, while other two regions (A and D) cannot be explained solely by the regional homology between the subunits. These regions were located at both junctions of the nucleosomal and the linker region, and overlapped with the binding motifs for alpha protein and CENP-B. Approximately 90% of the recombination occurred between the subunits located next but one (+/- 2 shift), although the frequency of recombination between the adjoining subunits (+/- 1 shift) was approximately 10%.
Base Sequence↗
Expression of the human cGMP-dependent protein kinase II gene is lost upon introduction of SV40 T antigen or immortalization in human cells.
We have cloned a human cGMP-dependent protein kinase type II cDNA to examine its gene expression in terms of cellular senescence and/or immortalization. The genetic locus was mapped to band 4q21 by FISH. Northern blot analysis revealed that expression of the type II gene was markedly decreased or lost in mortal or immortal human fibroblasts producing SV40 T antigen. Also in various immortalized cell lines tested, the gene was not expressed. In normal diploid fibroblasts, the gene was constitutively expressed during cell-cycle and population doubling levels (PDLs).
Amino Acid Sequence↗
Construction of libraries for methylation sites by in-gel competitive reassociation (IGCR).
The in-gel competitive reassociation (IGCR) procedure was successfully applied to construct a comprehensive library enriched in DNA fragments containing C5mCGG sequences from mouse liver and brain genomic DNA. For IGCR, methylation-insensitive restriction enzyme (Msp I) digests were used as target DNA and methylation-sensitive restriction enzyme (Hpa II) digests as competitor DNA. Southern blot analysis indicated that 60 to 70% of the clones in the library were derived from the methylated sites and overall enrichment was 200- to 1000-fold. IGCR was further applied to construct a library for the sites differentially methylated between brain and liver DNA. In the library, approximately 20% of the Hpa II sites exhibited different degrees of methylation between these tissues.
Animals↗
Chromosomal location of murine protein tyrosine phosphatase (Ptprj and Ptpre) genes.
It is now widely accepted that protein tyrosine phosphatases (PTPases) play important or even critical roles in cell growth, differentiation, and development. Our recent experiments suggested that specific PTPases, PTP beta 2 and PTP epsilon, are involved in the early molecular events for in vitro differentiation of mouse erythroleukemia (MEL) as well as embryonic carcinoma (F9) cells. Using mouse cDNA for PTP beta 2 and PTP epsilon, which we have cloned recently, we attempted to locate the genes to mouse chromosomes. Interspecific backcross analysis indicated that the gene for PTP beta 2, Ptprj, is located in the middle region of chromosome 2, and the gene for PTP epsilon, Ptpre, was mapped in the vicinity of the imprinted regions in the distal part of chromosome 7. Possible biological roles of these PTPases are discussed.
Alleles↗