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Biomedical subjects

M Ohno

Publications and source records attributed to M Ohno.

At least 631 records · Page 35Linked to original sources
PubMed1980

Properties of mesencephalic reticulospinal neurons in the cat.

Neurons that project to the spinal cord were located in the mesencephalic reticular formation outside the interstitial nucleus of Cajal in cerebellectomized cats under chloralose anesthesia. Of these neurons 40% responded only at C1 (reticulospinal N cells) and the remaining 60% responded at C4 also (reticulospinal D cells). Conduction velocities of N cells were significantly slower than those of D cells. N cells and D cells responded similarly to stimulation of the whole vestibular nerves and vestibular nuclei. However, they differ in semi-circular canal inputs; N cells were more responsive to canal stimulation. Comparison of properties between mesencephalic reticulospinal and interstitiospinal neurons (Fukushima et al. 1980) showed that many reticulospinal and interstitiospinal neurons have similar properties, suggesting that functionally similar neurons may be found distributed over more than one anatomically defined cell group.

Animals↗
PubMed1980

Serum coenzyme Q10 levels in thyroid disorders.

The correlation between serum CoQ10 levels and serum thyroid hormones in thyroid disorders was investigated in the present studies. Serum CoQ10 was measured by high speed liquid chromatography utilizing ultraviolet detector. In normal controls, serum CoQ10 level of the male was higher than that of the female. Serum CoQ10 level in hyperthyroidism was significantly lower than that of euthyroid subjects. But in hypothyroidism, serum CoQ10 level did not show any significant difference from that of euthyroid subjects. Significant inverse correlations were demonstrated between log CoQ10 and log T3, log T4, log free T4, or log rT3. These data suggested abnormalities in the mitochondrial electron transport system in thyroid disorders.

Adolescent↗
PubMed1980

Purification and properties of phospholipase A from venom of Trimeresurus flavoviridis (Habu snake).

Phospholipase A was purified from venom of Trimeresurus flavoviridis (Habu snake) via three steps consisting of Sephadez G-100, CM-cellulose, and DEAE-cellulose column chromatographies. The apparent molecular weights determined by gel filtration on Sephadex G-75 and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were 28,000 and 14,000, respectively, suggesting that the enzyme is composed of two identical subunits. The isoelectric point was 7.9. The enzyme is characterized by high contents of aspartic acid, glycine, tyrosine, and lysine and contains one residue each of histidine and methionine, three or four tryptophans, and eight disulfide bonds per subunit. The enzyme was inactivated by reaction with p-bromophenacyl bromide following pseudo first order kinetics. The loss of activity was accompanied by loss of histidine, indicating that a single histidine residue is essential for activity. Oxidation with N-bromosuccinimide decreased the enzymic activity. Tryptophan residues appear to play some role in catalysis.

Amino Acids↗
PubMed1980

Psoriasis occuring in young monozygotic twins.

Psoriasis occuring in monozygotic twins is reported. Although these patients had a different living environment since birth the similarity in psoriatic pattern and its time of onset suggested the strong influence of genetic factors. However the major histocompatibility antigens A2, A11, Bw(16), found in these patients were not those, which have been reported to be significantly increased among Japanese psoriatics.

Adult↗
PubMed1980

Effects of a new adenosine deaminase inhibitor, isocoformycin, on toxicity, antitumor activity and tissue distribution of formycin A and 9-beta-D-arabinofuranosyladenine.

Single intraperitoneal and intravenous injections of isocoformycin at 1,200 mg/kg did not cause the death of mice. Isocoformycin which inhibited adenosine deaminase enhanced significantly the toxicity of formycin A and ara-A at various combination ratios. Isocoformycin potentiated antitumor activity of formycin A and ara-A against L1210 leukemia. Formycin A and ara-A disappeared rapidly from the blood and tissues and could not be found in any tissues even 0.5 hour after a single intraperitoneal injection. However, when used in combination with isocoformycin both were detected in the blood and tissues, especially at high concentration in liver and kidney. These indicate that the deamination of formycin A and ara-A is blocked by isocoformycin in vivo.

Adenosine Deaminase Inhibitors↗
PubMed1980

[Immunological analysis of hydatidiform mole and invasive mole (author's transl)].

Thirty-two cases of hydatidiform mole without endometrial invasion of chorial tissue and 17 of invasive mole were studied to ascertain whether these two diseases can be differentiated by various immune parameters. All of hydatidiform 11 of invasive mole were sampled at a state of "mole in utero" (pre-evacuation), but 6 of invasive mole were at a post-evacuation state, respectively. The results were summarized in the following items; 1. Serum total protein, especially albumin decreased with no significant difference in both groups. Globulin fractionation (alpha 1, alpha 2, beta, gamma-globulin) showed no characteristic changes. 2. A variation of immunoglobin (IgG, IgM, IgA) was similar in both. 3. Beta 1 C (a component of complement) increased in two groups, but no difference was revealed. 4. Leucocytosis was observed, but lymphocytosis was not detected in both groups. 5. Active E-RFC slightly increased, although total E-RFC and EAC-RFC were within normal range. But these findings were not statistically different in both. 6. In vitro lymphocyte-PHA-response was fairly depressed without a significant difference in two groups. 7. Suppressive effect of serum on lymphocyte-response was also slightly strengthened, but a difference in both was not significant. 8. The results of immune skin tests (PHA, PPD, SK-SD, Candida) could not be debated with liquid numbers studied.

Blood Proteins↗
PubMed1980

[Studies on mixed lymphocyte-tumor cell culture reaction (MLTR) in gynecological malignant tumors by the microplate methods (author's transl)].

For the purpose of observing the cellular immune response to their own tumor cells, MLTR was performed on 16 cases of cervical cancer, 3 cases of corporeal cancer and 6 cases of ovarian cancer. The results of MLTR was presented as a stimulation index (S.I.) by the following formula. (Formula: see text). Results of S.I. were as follows; 1.37 +/- 1.47 in cervical cancer, 0.65 +/- 0.21 in corporeal cancer and 1.12 +/- 0.74 in ovarian cancer. A positive S.I. case which was more than 2.0, was found in 3 cases with cervical cancer (18.8%), in none with corporeal cancer (0.0%) and in 2 with ovarian cancer (33.3%). Thus, the cellular immune response to their autologous tumor cells was considered to be very slight in this assay system.

Female↗
PubMed1980

The interaction of the fluorescent probe 1-anilinonaphthalene-8-sulfonate with Carlsberg subtilisin.

1-Anilinonaphthalene-8-sulfonate (Ans) binds to Carlsberg subtilisin [EC 3.4.21.14] with a large enhancement of its fluorescence intensity and a shift of the emission maximum to shorter wavelength. The present study indicated that one molecule of Ans binds to Carlsberg subtilisin and inhibits the hydrolysis of substrates in a noncompetitive manner. The dissociation constants of Ans-Carlsberg subtilisin complex were 6.5 x 10(-4) M at pH 6.5 and 7.8, respectively, in terms of fluorescence titration, being in accord with the values 5 approximately 8 x 10(-4) M at pH 7.8) obtained from kinetic studies using various substrates. The dissociation constant of N alpha-acetyl-2-(2-nitro-4-carboxyphenylsulfenyl)-L-tryptophan methyl ester (Ac-Trp(NCps)-OMe), which is a competitive inhibitor of the enzyme, however, became 3.3 times greater in the presence of Ans. It was also observed that the fluorescence intensity of the Ans-enzyme complex decreased in the presence of Ac-Trp(NCps)-OMe or N alpha-acetyl-O-trans-p-phenylazobenzoyl-L-tyrosine methyl ester (Ac-Tyr(PABz)-OMe). These phenomena suggest that the Ans binding site is in the vicinity of the active site of the enzyme.

Anilino Naphthalenesulfonates↗
PubMed1980

[Sensitization of lymphocyte to tumor extract detected by macrophage migration inhibition test(MIT) in patients with cervical cancer (author's transl)].

In vitro cellular hypersensitivity to the tumor-associated antigens (TAA) in 30 patients with cervical cancer (12 cases with stage 1b and 18 cases with stage 11) was studied by the method of macrophage migration inhibition test (MIT). Tumor soluble extracts as antigen were obtained by sonication or 3M-KCL method. The results of MIF production was presented as a migration index (MI) by the following formula. (Formula: see text), The result of MI were as follows: 79.25 +/- 21.69 in patients with stage 1b, 79.50 +/- 14.65 with stage 11. A positive MI case (MIF positive) which was less than 75, was found in 8 cases (66.7%) with stage 1b and 5 cases (27.8%) with stage 11. Relationship between the result of MI and skin reactions were as follows: 75.83 +/- 17.30 in PPD positive (18 cases), 84.75 +/- 16.96 in PPD negative (12 cases); 78.70 +/- 18.24 in DNCB positive (20 cases), 84.75 +/- 16.96 in DNCB negative (10 cases); 77.93 +/- 15.79 in PHA positive (27 cases) and 97.33 +/- 24.79 in PHA negative (3 cases). The serial assay of MI were performed in 10 cases and MIF production disappeared within 6 weeks after curative operation in all cases. From these findings, the cellular immune response to autologous tumor extracts was found in this assay system.

Antigens, Neoplasm↗
PubMed1979

Isolation and characterization of human placental chorionic villar extracellular matrix.

The cell-free extracellular matrix of human placental chorionic villi has been prepared by a procedure employing extraction of the terminal villar fragments with the detergents Triton X-100 and sodium deoxycholate. The isolated human placental extracellular matrix retains an intact, but collapsed, histoarchitecture, as observed by scanning and transmission electron microscopy. It remains intact, in large part because of the presence of continuous sheets of villar basement membranes and associated interstitial collagen fibers and scattered patches of fibrin. The staining charcteristics and chemical composition of the isolated human placental extracellular matrlix are similar to those reported for basement membranes in several tissues and indicate the presence of collagen-like and glycoprotein components in this preparation. Gel electrophoresis of urea-SDS-mercaptoethanol extracts of the matrix showed that it consists of several polypeptide components of various molecuar weights, some of which are associated into high molecular weight complexes by disulfide bonds.

Amino Acids↗
PubMed1979

Body surface potential distributions in posterior ventricular pre-excitation.

Waveform of the QRS complex during ventricular pre-excitation is subject to the influence of both the site of pre-excitation and the time of pre-excitation relative to that of excitation via the normal AV path. This paper reports a case in which lead V1 of the electrocardiogram (ECG) could be altered from an R to an rS pattern by the administration of atropine sulfate. The provable mechanism was that of reduced conduction time in the normal AV path with altered time phase of normal excitation and pre-excitation. This mechanism was simulated in experiments on dogs and yielded similar findings. Body surface mapping in both the patient and the dogs provided evidence that pre-excitation could be recognized by that means with varied time phase of normal excitation and pre-excitation. It was demonstrated that the QRS complex of right sided precordial leads could be altered from an R to an rS pattern by altering the time phase of normal excitation and pre-excitation of the posterior ventricular wall. This alteration was related to the degree to which negative potentials on the anterior chest wall due to right ventricular breakthrough of normal activation developed in relation to the time of pre-excitation.

Action Potentials↗