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Biomedical subjects

M Ohashi

Publications and source records attributed to M Ohashi.

At least 415 records · Page 23Linked to original sources
PubMed1984

Silver stain for proteins on a cellulose acetate membrane.

A rapid and sensitive silver staining method to detect proteins on a cellulose acetate membrane has been established. This method is achieved by modification of the silver-based color staining for detection of proteins in polyacrylamide gels [D. W. Sammons, L. D. Adams, and E. E. Nishizawa, Electrophoresis 2, 135-141 (1981)] and applied to our new type of two-dimensional electrophoresis for analysis of proteins on a cellulose acetate sheet [T. Toda, T. Fujita, and M. Ohashi, Anal. Biochem. 119, 167-176 (1982)]. Maximal sensitivity of silver stain for proteins on a cellulose acetate membrane can be obtained by an optimal balance between deposition of silver on the protein and on the background. Certain kinds of proteins are colored red, orange, or grayish-blue. The silver stain is 20-80 times more sensitive than Coomassie blue and some spots are visualized reproducibly by silver only. Densitometric evaluation of standard proteins stained with silver and Coomassie blue is also demonstrated. The method takes only 50 min to perform and is sensitive, simple, and reproducible.

Animals↗
PubMed1984

A comparison of the tensile bond strengths of composite resins to longitudinal and transverse sections of enamel prisms in human teeth.

The tensile bond strengths of composite resins to transverse and longitudinal sections of enamel prisms etched with phosphoric acid were examined. The tensile bond strengths were 18-19 MPa to the transverse and 10-11 MPa to the longitudinal sections and thus were influenced by the nature of the enamel crystal planes. These facts suggest that composite resin restorations will have a longer life span if the enamel walls are given marginal forms to produce transverse rather than longitudinal sections of enamel prisms.

Acid Etching, Dental↗
PubMed1984

[Treatment of esophageal cancer by endoscopic administration of peplomycin ointment].

Pepleomycin ointment was developed to improve the antitumor activity afforded by endoscopic therapy for esophageal cancer. Through the esophagoscope we could observe this ointment specifically attacking the lesion. Furthermore we were able to recognize that peplomycin was acting directly on the lymph nodes as well as the tumor in a resected portion of the esophagus. It is hopefully anticipated that this method will be used as a combination therapy in pre-operative and inoperable esophageal cancer.

Aged↗