Adrenal function and alcoholism. II. Catecholamines.
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Adrenal Glands↗
Biomedical subjects
M Ogata
Publications and source records attributed to M Ogata.
Effect of dentin primer application on regional bond strength to cervical wedge-shaped cavity walls.
The purpose of this study was to examine the effect of multiple applications of self-etching primer on regional tensile bond strength (microTBS) to artificial wedge-shaped cavities (i.e., occlusal vs gingival wall). Eighteen extracted noncarious human molars were used to measure microTBS, and four additional teeth were used for scanning electron microscopy (SEM) of the interface. Wedge-shaped defects were prepared in the buccal cervical dentin. The teeth were mounted on their distal surface, simulating the supine position of the teeth during dental treatment. The teeth were divided into two groups according to the bonding systems [Clearfil Liner Bond II (LB) or Imperva Fluoro Bond (FB)], and again divided into two subgroups for bonding. One group (Group S) was treated once with the primer according to the manufacturer's instructions. For the other group (Group M), the primer was applied several times for the period recommended by the manufacturer. The adhesive bonding resins were then applied to the cavity walls, and restored with a low-viscosity resin composite (Protect Liner F), and stored in 37 degrees C water for 24 hours. The resin-bonded teeth were serially sliced parallel to the long axis of the tooth. The adhesive interface of each slice was trimmed alternately at the occlusal or gingival wall, and a microtensile testing method was used to compare resin bond strength to each wall. With both adhesive systems and primer application methods, tensile bond strength to the gingival wall was significantly lower than to the occlusal wall (P < 0.05). Tensile bond strength of LB to each cavity wall of a wedge-shaped defect increased significantly by multiple primer application (P < 0.05); however, bond strengths of FB were not significantly different after multiple primer applications.
Analysis of Variance↗
Influence of the direction of tubules on bond strength to dentin.
This study investigated the influence of the direction of dentinal tubules on resin-dentin tensile bond strength (mu TBS) using four commercially available bonding systems and observed the resin-dentin interfaces with an SEM. The dentin bonding systems used in this study were Clearfil Liner Bond II (LB, Kuraray), Imperva Fluoro Bond (FB, Shofu), Single Bond (SB, 3M) and One-Step (OS, BISCO). Thirty-six extracted caries-free human molars were used for micro tensile bond testing and eight additional teeth were used for scanning electron microscopy (SEM). The teeth were divided into two groups according to the direction of the dentinal tubules at the resin-dentin interface: a perpendicular group, in which the occlusal enamel was removed perpendicular to the long axis of the tooth, and a parallel group, in which the mesial half of the tooth was removed parallel to the long axis of the tooth, and the coronal dentin surface was used for bonding. After the flat dentin surfaces were polished with #600 silicon carbide paper, each surface was treated with one of the four adhesive systems according to the manufacturer's recommendation, then covered with resin composite (Clearfil AP-X, Kuraray) to provide sufficient bulk for micro-tensile bond testing. After 24 hours in 37 degrees C water, the resin-bonded teeth were serially sliced perpendicular to the adhesive surface, the adhesive interface trimmed to a cross sectional area of 1 mm2 and subjected to tensile forces at a crosshead speed of 1 mm/min. Statistical analysis of the tensile bond strengths were performed using two-way ANOVA and Fisher's PLSD test at 95% level of confidence. The tensile bond strength of the group with tubules parallel to the bonded interface was higher than that of tubules cut perpendicularly. This tendency reached statistical significance using SB and OS.
Analysis of Variance↗
The effect of additional enamel etching and a flowable composite to the interfacial integrity of Class II adhesive composite restorations.
This in vitro study evaluated the interfacial integrity of Class II resin composite restorations. The influence of a flowable composite and additional enamel etching was also evaluated. Deep, saucer-shaped Class II cavities were prepared in the mesial and distal proximal surfaces of 25 extracted human molars and assigned to five treatment groups. The gingival margins were extended to approximately 1 mm above the CEJ in 40 cavities and below the CEJ in 10 cavities. The prepared cavities were then restored with a self-etching primer system (Clearfil Liner Bond II) and a hybrid resin composite (Clearfil AP-X), with and without a flowable composite (Protect Liner F) and additional enamel etching with 37% phosphoric acid gel (K-etchant). After finishing, polishing and thermocycling (4 and 60 degrees C, x300), the samples were longitudinally sectioned through the restorations and resin-tooth interfaces were observed directly under a laser scanning microscope. Statistical analysis indicated that the use of a flowable composite produced significantly more (p = 0.04) gap-free resin-dentin interfaces than teeth restored without the flowable composite. However, both flowable composite and enamel etching could not prevent gap formation at enamel-resin interfaces and crack formation on enamel walls.
Acid Etching, Dental↗
Effects of different burs on dentin bond strengths of self-etching primer bonding systems.
This study evaluated the effects of cutting dentin with different types of burs on tensile bond strength using three self-etching primer bonding systems (Clearfil Liner Bond 2 [LB2], Clearfil Liner Bond 2V [2V] and Clearfil SE BOND [SE], Kuraray, Co, Ltd, Osaka, Japan). Thirty-six intact extracted human third molars were ground flat to expose occlusal dentin, followed by polishing the dentin surfaces with #600 SiC paper. The teeth were divided into four groups according to bur type and grit: fine cut fissure steel bur (SB600), cross cut fissure steel bur (SB703), regular grit diamond bur (DB). Controls were abraded with #600 grit SiC paper (AP#600). The dentin surfaces of the SB600, SB703 and DB groups were cut under copious air-water spray with the respective burs mounted in a dental handpiece. The teeth were treated with one of three adhesive systems, then composite buildups were created with Clearfil AP-X (Kuraray Co, Ltd, Osaka, Japan). After soaking in water at 37 degrees C for 24 hours, serial vertical sections (0.7 mm thick, 7-8 slices per one tooth) were made, trimmed to form an hour-glass shape with a 1.0 mm2 cross-section and tensile bond strengths were determined at a cross-head speed of 1 mm/min. Statistical analysis was made using one and two-way ANOVA and Fisher's PLSD test (p<0.05). Eight additional molars were prepared. Burs or abrasive paper were used for SEM observations of the dentin surfaces of each group before and after treatment with the self-etching primers. All adhesive systems yielded the same ranking of bond strengths to the surfaces prepared with different abrasives: from highest to lowest, AP#600 > SB600 > SB703 > DB. This ranking reached statistical significance using Clearfil Liner Bond 2V (p<0.05). Therefore, when cutting dentin, selecting the adequate bur type is important for improved bonding of adhesive systems using self-etching primer to dentin.
Acid Etching, Dental↗
Effect of self-etching primer vs phosphoric acid etchant on bonding to bur-prepared dentin.
This study evaluated the effect of dentin conditioner on tensile bond strength to dentin prepared with different types of burs. A self-etching primer system, Mac-Bond II (MB, Tokuyama Dental) and a phosphoric acid etching system, Single Bond (SB, 3M) were used for conditioning. Twenty-four extracted intact human molars were ground flat to expose occlusal dentin. After the dentin surfaces were polished with #600 SiC paper, the teeth were randomly divided into a control group and three experimental groups according to the bur grits used: #600 SiC paper only as the control, fine cut steel bur (SB600), crosscut steel bur (SB703) and regular grit diamond bur (DB) mounted in a dental handpiece utilizing water cooling. The dentin surfaces were treated with one of two adhesive systems, then composite buildups were done with Clearfil AP-X (Kuraray Medical). After soaking the bond specimens for 24 hours in 37 degrees C water, multiple vertical serial sections (0.7 mm thick, 7-8 slices per one tooth) were made, trimmed to form an hour-glass shape with a 1.0 mm2 cross-section and tensile bond strengths were determined at a crosshead speed of 1 mm/minute. Statistical analysis was made using one and two-way ANOVA and Fisher's PLSD test (p<0.05). Six additional molars were used for SEM observations of the dentin surfaces of each group before and after treatment with the self-etching primer of MB, and another four teeth were used to observe the resin-dentin interface of each group of SB. Using MB, the DB group produced the lowest tensile bond strength (TBS) among the groups that received bur preparation, and there were no statistical differences among SB600, SB703 and the control. For SB, the TBS of SB703 was the highest, and there were no statistical differences among the other groups and the control. The influence of the method used to prepare dentin for micro-tensile bond strength testing was dependent on the adhesive system used.
Acid Etching, Dental↗
[Parendodontal surgery of the lower third molar: report of a clinical case].
The parendodontic surgery intends to the resolution of accidental or pathological condition that can't be recoverable by usual endodontic treatment. In this case, the authors present a clinical case where a apical curettage and apicectomy were done in a mandibular right third molar, to show the account of this procedure yet in the actual context.
Adult↗
Proliferation and differentiation of immature thymocytes induced by a thymic stromal cell clone.
The monolayer of our established thymic stromal cell clone (MRL104.8a) exhibited the capacity to maintain immature double-negative thymocytes. Such capacity was also expressed by a factor produced by the MRL104.8a monolayer. This factor designated as thymic stroma-derived T cell growth factor (TSTGF) was found to be distinct from IL-2 or IL-4 but similar to IL-7 of the previously described cytokines from the functional and molecular aspects. The MRL104.8a monolayer also exerted its differentiation-promoting effect on double-negative thymocytes. Culture for one day of purified double-negative thymocytes on the monolayer resulted in the induction of an appreciable per cent of CD3-4-8+ cells. This differentiation could also be induced by a semipurified TSTGF sample but not by recombinant IL-7, suggesting that the MRL104.8a cells elaborate a factor(s) responsible for initiating the differentiation of double-negative cells in addition to the growth-promotion factor identical or closely related to IL-7. When the culture period of double-negative thymocytes was extended to 2 or 3 days, an appreciable number of double-positive (CD4+8+) and single-positive (CD4+8-) cells were generated on the MRL104.8a monolayer. Thus, these observations provide strong support for the proposition that a specialized thymic stromal component plays an essential role in the intrathymic T cell development in the context of T cell growth and differentiation.
Animals↗
Excision of papillary endocardial tumor.
We report the eighth case of surgical removal of a papillary endocardial tumor. The surgical approach was aided by intraoperative two-dimensional echocardiography. The tumor was attached to the chorda tendinea of the mitral valve and was successfully removed via a left atrial approach.
Adult↗
Pasteurellosis in Japan: recent progess in the research of Pasteurella multocida in this country.
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Animals↗
Changes in alveolar capillary formation in growing rat lung by repeated injections of a lathyrogen.
We hypothesized that abnormal capillary formation, which might be associated with an alteration in extracellular malular matrix (ECM), occurs in the alveoli of growing rat lungs treated with beta-aminopropionitrile (beta APN), a lathyrogen that inhibits lysyl oxidase activity. On scanning electron microscopy, a corrosion cast of alveolar capillaries in lungs treated with beta APN appeared abnormal in configuration; transmission electron microscopy showed extensive morphological changes in interstitial cells and ECMs, including collagen, elastin, and presumably glycosaminoglycans and, in binding water (GBW). Morphometric data revealed an increase in GBW of up to 24.4%, a decrease in the amount of collagen fiber (44.5%), and a decrease in lipid-laden interstitial cells; however, the change in elastin was limited to morphological appearance. From these observations, we conclude that alterations in alveolar capillary formation are associate with extensive changes in ECMs, and that these changes in ECM components might also be involved in the abnormal alveolar formations induced by beta APN.
Aminopropionitrile↗
The significance of motion sickness in the vestibular system.
In order to clarify the significance of motion sickness in the vestibular system, we compared the findings of experimental motion sickness between different kinds of subjects, some of which were already reported. Subjects were healthy adults, healthy children between the ages of 4 and 15 years, and patients with congenital and acquired labyrinthine loss. They were asked to walk while wearing horizontally and vertically reversing goggles. Equilibrium ataxia as well as motion sickness were evoked by horizontal reversal, but not by vertical reversal in healthy subjects. Kindergarten children exhibited severe ataxia, but little nausea. The frequency of severe ataxia decreased during growth, inversely as the frequency of nausea syndrome increased. Although a patient with acquired loss became severely ataxic, a patient with congenital loss did not show any ataxia at all. The present study suggests that vestibular cues are indispensable to the ego-spatial relationship in the brain, and once the ego-spatial relationship becomes inadequate, discomfort acts as a safety device to brake uncontrollable actions. Then, perception of the outer world may automatically adjust voluntary actions by affecting motor commands. The importance of visual cues for representing an alternative framework may differ between congenital and acquired labyrinthine loss.
Adolescent↗