Biomedical subjects

M Oehmichen

Publications and source records attributed to M Oehmichen.

At least 163 records · Page 9Linked to original sources

PubMed1976

Reactive microglia with membrane features of mononuclear phagocytes.

An inflammatory response was induced by the implantation of cover-slips into the brains of rabbits. The cytomorphology of the glass-adhering cells, their phagocytic properties and their enzymatic activity were characterized and these features were correlated with the presence of membrane markers. Mononuclear cells with foamy cytoplasm, with marked phagocytic potential and strong activity of nonspecific esterases consistently showed IgG- and C-receptor activity. Multinuclear giant cells, which increased in number after prolonged implantation, exhibited comparable features. In contrast, mononuclear cells of fusiform appearance with a low activity of nonspecific esterases and a poor phagocytic potential lacked the IgG-receptor. A close relationship between the mononuclear phagocytic cell and reactive microglia is postulated. The authors conclude that the inflammatory response observed under their test conditions was characterized by the prevalence of phagocytic cells with membrane characteristics of the monocyte-macrophage series.

Acid Phosphatase↗

PubMed1976

Receptor activity on some mesenchymal cells in CNS of normal rabbits. Indications of the monocytic origin of intracerebral perivascular cells, epiplexus cells and mononuclear phagocytes in the subarachnoid space.

The surface receptor activity for various cell types within rabbit CNS was investigated. Sheep red blood cells (E) used as markers were washed, sensitized with the IgG-fraction of E-antibodies (EA) or additionally coated with complement (EAC) and incubated with CNS cells. The inhibitory effect produced by the addition of soluble IgG was investigated. Incubation (1 h) with red cells was undertaken in three ways: 1. Rabbit leptomeninx was stripped and incubated. 2. Rabbits were killed, the brain was perfused with warm buffer and red cell complexes were then injected intracerebrally, intrathecally and intraventricularly into the perfused brains. 3. E, EA and EAC were injected intracerebrally, intrathecally and intra-ventricularly into living rabbits. Using these methods, receptor sites for IgG and complement of mononuclear cells from the subarachnoid space, epiplexus cells and perivascular cells from intracerebral vessels could be demonstrated. In other areas of the body, these receptors have been demonstrated using similar methods only on cells from the monocyte-macrophage series. The common derivation of these three cell types from the monocytes as well as their comparable function within the immune response is discussed.

Animals↗

PubMed1976

[Histochemical studies on innervation of the distal ureter in primary vesico-ureteral reflux in children (author's transl)].

The purpose of the present investigation was to evaluate the distribution-pattern of the intramural vegetative nerve fibres in cases of vesico-ureteral reflux. Resected specimens of the vesico-ureteral junction of 14 children with primary vesico-ureteral reflux are investigated using histochemical technics for demonstrating acetylcholinesterase (El Badawi and Schenk, 1967). Additionally the catecholamines (Falck et al. 1962) were demonstrated in two of the cases. The results are compared with findings in normal ureters in literature. There was no evidence of a pathological pattern of distribution of the nerve fibres.

Acetylcholinesterase↗

PubMed1976