Sleep disorders. Consider nocturnal paroxysmal dystonia.
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Dystonia↗
Biomedical subjects
M Oakey
Publications and source records attributed to M Oakey.
Sleepwalking, night terrors, and consciousness.
OBJECTIVE: To determine some personality and psychoneurotic characteristics of adults who have the sleepwalking-night terrors syndrome. DESIGN: Prospective assessment of two groups of consecutive patients with a firm diagnosis of either of two specific sleep disorders as established clinically and by polysomnography. SETTING: Outpatient sleep disorders clinic and sleep laboratory in a tertiary referral centre. PATIENTS: 12 Patients referred consecutively to the clinic in whom a diagnosis of sleepwalking (six) or night terrors (six) was confirmed. MAIN OUTCOME MEASURES: Psychological characteristics as measured at the time of clinical assessment by means of the Eysenck personality questionnaire, the hostility and direction of hostility questionnaire, and the Crown-Crisp experiential index. RESULTS: Both groups scored exceptionally highly on the hysteria scale of the Crown-Crisp experiential index and the night terrors group also scored highly on the anxiety scale. The patients with sleepwalking also scored highly on a measure of externally directed hostility. CONCLUSIONS: The physiological and psychological features identified in these patients, possibly reflecting different expressions of a constitutional cerebral characteristic, may be explored in terms of hysterical dissociation. The findings contribute to the debate concerning the nature of sleepwalking, in particular with and without the forensic aspects.
Adult↗
A monoclonal antibody based solid-phase enzyme-binding assay to measure levels of placental alkaline phosphatase in serum of women during pregnancy.
Monoclonal antibodies raised against highly purified placental alkaline phosphatase (PALP) reacted specifically with PALP and were found not to cross-react with the liver, kidney and intestinal enzymes. One of these antibodies (AP-3) was selected to develop an enzyme-binding assay to measure levels of PALP in the sera of 11 women throughout pregnancy. PALP activity rose sharply after 28 weeks of gestation reaching a peak at delivery followed by an immediate drop within 4 days to values approaching non-pregnant levels. The potential of using this assay as an index of placental function to monitor progress of a pregnancy is discussed.
Alkaline Phosphatase↗
Use of borate buffers for purification of placental beta-glucuronidase on DEAE-cellulose.
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Borates↗
Purification and properties of human placental cathepsin D.
Human placental cathepsin D has been purified 6000-fold and its properties characterized. Its molecular weight has been ascertained to be 42 000 by gel filtration and 43 300 by analytical ultracentrifugation. SDS gel electrophoresis in the presence of beta-mercaptoethanol cleaves the enzyme into two polypeptides of molecular weights 28 200 and 14 400. The placental enzyme resembles cathepsin D isolated from other mammalian tissues in many of its properties, including pH optimum. The higher degree of purification has led to a shift in the isoelectric points of the three isoenzymes from those recorded by other authors. Antibodies raised against cathepsin D in rabbits inhibit it at pH 5.0, and the inhibition is almost 100 per cent with adequate concentrations of monospecific antibody.
Antibodies↗