Biomedical subjects

M Nowak

Publications and source records attributed to M Nowak.

At least 181 records · Page 10Linked to original sources

PubMed1983

A low molecular weight immunosuppressive factor produced by Onchocerca gibsoni.

An immunosuppressive factor(s) which is heat stable, and dialysable, with a molecular weight less than 10,000 molecular weight, and is not species specific, has been shown to be produced during the in vitro culture of Onchocerca gibsoni microfilariae. The factor(s) is capable of abolishing lymphocyte mitogenesis induced by Con A in bovine lymphocytes and PHA in human lymphocytes. The factor(s) may play a role in establishing the microfilariae in the tissues of the host by depressing cell mediated immune reactions during invasion.

Animals↗

PubMed1982

Management of vascular injuries to the leg.

Our experience with 69 vascular injuries in 56 patients led us to modify the management of vascular injuries to the leg. We believe that prompt and complete angiography whenever the general condition of the patient allows it, early fasciotomy when indicated before vascular repair, thrombectomy of the injured artery and vein and local instillation of heparinized saline solution, vascular repair before orthopedic stabilization of fractures in selected cases, external fixation of the fracture when there is significant soft tissue injury, and early skin grafting resulted in an improved level of care with a low morbidity and no mortality in our series.

Adolescent↗

PubMed1982

Production of high titres of interferon-gamma by cells derived from short-term cultures of murine spleen leukocytes in T-cell growth factor-conditioned medium.

Short-term culture of activated murine spleen cells in T-cell growth factor-conditioned medium resulted in a cell population that produced high titres of interferon-gamma in response to the T-cell mitogen concanavalin A. Compared to interferon-gamma production by freshly isolated spleen cells in response to the same mitogen, titres were 10 to 20 times higher at all cell densities tested. Furthermore, the minimal cell density at which interferon-gamma production could be detected was significantly lower. The mitogen dose required for optimal interferon induction in this system was higher than in conventional spleen cell cultures.

Animals↗

PubMed1982

Adhesion and locomotion of L5222 cells on endothelium, collagen and glass.

Examination was made of the adhesive interaction of L5222 leukemia cells with endothelial cells, collagen and glass and of cell locomotion on endothelium and collagen. Leukaemia cells interacted with the substrate under stationary conditions. The fraction of cells adherent to the substrate was defined next, using the given shearing force caused by the medium flowing through the measuring channel. The relative number of adherent cells, A (related to the number of cells after sedimentation), remaining on the given surface despite the detaching action of shearing force, F, was determined. The range of F values applied was 0.1 to 30 (x 10(-13)) N. It was found that the relation A(F) is a decreasing function for all the substrates examined and takes on values in the relation 1:2:6 for cells adherent to collagen, glass and endothelium, respectively. The critical value of F, at which values of A are maintained at a constant level close to zero, was 0.5, 1 and 3 (x 10(12)) N, respectively, for cells adherent to these substrates. The function A(F) for L5222 cells adherent to endothelial cells and to glass can be described well by the formula: A = a/square root F (where a = constant greater than 0). Studies of L5222 cell locomotion on an endothelial cell layer and on collagen revealed that the pattern of locomotion, variations in locomotion velocity and the mean values of cell displacement (5.8 and 5.0 micrometers, respectively) are similar for both substrates.

Animals↗

PubMed1982