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Biomedical subjects

M Noguchi

Publications and source records attributed to M Noguchi.

At least 721 records · Page 40Linked to original sources
PubMed1984

[Important roles of oocytes, follicles and corpora lutea in ovarian teratocarcino-genesis in mice].

In strains LT/Sv and LTXBJ mice, susceptible to spontaneous ovarian teratocarcinogenesis, ovarian teratomas are derived from parthenogenetically activated ovarian eggs. The follicles containing the parthenotes are the abnormal ones in which granulosa cells show histochemically high activity of beta-glucuronidase (beta-G). To investigate whether ovarian teratocarcinogenesis depends on the genetic nature of eggs themselves or the microenvironment in the abnormal follicles, we used chimeras derived from aggregates of eight-cell embryos of strains LTXBJ or (LTxLTXBJ) F1 and of normal nonsusceptible strain C3H/HeJ which shows low activity of beta-G genetically. The original strain of teratomas and follicles in chimeras were histochemically determined by activity of beta-G as a marker. The results showed that both teratomas and the follicles containing parthenotes originate from the susceptible strains. Then, we examined for presence of activated oocytes, teratomas and corpora lutea in LT ovaries untreated or treated with PMSG-HCG implantation. These results showed that parthenogenetic blastocysts closely adhere to the follicle tissue as if a kind of and develop to post implantation stages in the ovaries in which corpora lutea were formed near the parthenotes. In conclusion, it was suggested that ovarian parthenogenesis in mice occurs most frequently when eggs with genetically susceptible nature, were exposed to the microenvironment produced by the abnormal follicles, and these parthenotes adhere to the ovarian tissues and differentiate three germ layers by some roles of corpora lutea and undergo teratocarcinogenesis.

Animals↗
PubMed1983

[The cellular mechanism of action of gastrin on the corporal mucosa of the canine stomach. (2) Ca2+-activated, phospholipid-dependent protein kinase and phospholipid turnover--possible mediator of gastrin action].

The existence of Ca2+-activated, phospholipid-dependent protein kinase (protein kinase C), the effect of gastrin on phospholipid metabolism and guanylate cyclase activity were investigated to elucidate the cellular mechanism of action of gastrin on the corporal mucosa of the canine stomach. Protein kinase activity was determined by measuring the incorporation of [32P] into calf thymus H1-histone from [32P]-ATP. One unit of protein kinase was defined as the amount of enzyme which incorporated 1 pmol of phosphate from ATP into H1-histone. Protein kinase C was found in 100,000xg supernatant of homogenate fractionated by a DEAE-cellulose column chromatography. Characteristics of further purified protein kinase C, such as dependency on divalent cations and phospholipids, were in agreement with those of previously reported protein kinase C in other tissues. Furthermore, the gastric corporal mucosa was found to contain protein kinase C in large quantities. The specific activity of protein kinase C was 26,000 units/mg protein. The phospholipid metabolism was evaluated by the incorporation of [14C]-glycerol-3-phosphate and the change of the radioactivity of [32P] in individual phospholipids. Each phospholipid was extracted from the gastric corporal mucosa and isolated by thin layer chromatography. Guanylate cyclase activity was determined by measuring the cGMP produced, using radioimmunoassay. Gastrin significantly increased the incorporation of [14C]-glycerol-3-phosphate into phosphatidylethanolamine in the presence of acetylcholine (Ach). Ach increased the uptake of the tracer into phosphatidylinositol significantly, and the increase was enhanced by the simultaneous addition of gastrin. In the experiments with [32P]-labeled phospholipids, gastrin increased the incorporation of [32P] into phosphatidylethanolamine significantly. The significant increase of the radioactivity in phosphatidylinositol by Ach failed to be enhanced by gastrin, but that of phosphatidylethanolamine by Ach was enhanced by gastrin. No stimulation of guanylate cyclase activity by gastrin was detected in the dispersed gastric corporal mucosal cells. These results indicate that gastric corporal mucosa was one of the most abundant tissues in which protein kinase C was contained, when compared with various mammalian tissues previously reported by Minakuchi, Nishizuka, et al. Nishizuka et al, recently proposed the novel hypothesis that phosphatidylinositol turnover activated by cAMP-independent agonists will be essentially required to activate protein kinase C. Our results suggest that gastrin can provoke phospholipids turnover including phosphatidylinositol turnover in gastric corporal mucosa. Therefore, our data indicate the possibility that the protein kinase C system plays an important role in the cellular mechanism of action of gastrin on gastric corporal mucosa.

Animals↗
PubMed1983

Serotonin-induced contraction of canine basilar artery: mediation by 5-HT1 receptors.

Serotonin and related agonists cause a marked contraction of canine basilar artery segments. These contractions can be inhibited by a variety of serotonergic antagonists. Absolute potencies of both serotonin agonists and antagonists in chamber contraction studies significantly correlate (P less than 0.01) with their potencies for 5-HT1, but not 5-HT2, receptors as defined by central nervous system radioligand binding studies. Serotonin-induced contractions of canine basilar artery segments appear to be mediated by 5-HT1 receptors.

Animals↗
PubMed1983

Intracystic carcinoma of the breast in an elderly man.

An eighty year old man with a mass in the left breast visited our department on October 27, 1980. Examination disclosed a smooth surfaced, round, elastic soft, 3 cm in diameter sized, non-tender mass underneath the left nipple. Mammogram showed a well defined oval opacity and echogram showed a cystic lesion. The mass was punctured and dark brown fluid was aspirated, but there were no malignant cells. The cyst was extirpated and a rice grain sized mass was found in the cyst wall. Histologically, it was diagnosed as non-infiltrating medullary tubular carcinoma. On November 27, 1980, simple mastectomy revealed no histological evidence of a residual cancer.

Adenocarcinoma↗
PubMed1983

Radical mastectomy with intrapleural en bloc resection of internal mammary lymph node by sternal splitting.

An operative method of extended radical mastectomy involving intrapleural en bloc resection of the internal mammary lymph nodes by sternal split was proposed. The operation enables complete resection of axillary and internal mammary lymph nodes. Seventy patients with primary breast cancer underwent the extended operation and two patients with recurrence in the internal mammary lymphnode chain following standard radical mastectomy underwent resection of internal mammary lymphnodes by sternal split, with no fatalities and no increase in postoperative disabilities. Metastases to the internal mammary lymphnode chain were histopathologically found in 14 of 70 patients with primary breast cancer and in two with recurrence in the internal mammary lymphnode chain, following standard radical mastectomy; The location of the metastatic internal mammary lymphnodes was from just below the subclavicular vein to the third intercostal space along the internal mammary vessels. Cancer cells were seen not only in the lymphnodes, but also in lymphatics of areolar tissue near the node or in lymphatics between the parietal pleura and endothoracic fascia in patients with primary breast cancer. And cancer invasion to parietal pleura was seen in patients with recurrence in the internal mammary lymphnode chain following standard radical mastectomy. All these findings indicate the rationality of our extended procedures.

Adult↗
PubMed1983

A stoichiometric study of heme degradation catalyzed by the reconstituted heme oxygenase system with special consideration of the production of hydrogen peroxide during the reaction.

In heme degradation catalyzed by the reconstituted heme oxygenase system, 8 to 9 mol of dioxygen and 11 to 12 mol of NADPH were consumed per mol of hemin lost, and about half the amount of dioxygen consumed could be accounted for by the production of hydrogen peroxide, which accumulated in the reaction mixture. Production of hydrogen peroxide in the heme oxygenase reaction did not appear to be due to the bimolecular dismutation of superoxide anions but rather seemed to be due to dissociation of a "peroxo" species formed on heme or intermediates of heme degradation. The hydrogen peroxide produced appeared to cause a considerable degree of non-specific degradation of heme (not leading to the formation of biliverdin) and also caused an inactivation of heme oxygenase. By taking into account the amount of dioxygen incorporated into hydrogen peroxide and some other factors, it could be deduced that 3 mol of dioxygen is consumed for the formation of 1 mol of biliverdin in the heme oxygenase reaction.

Animals↗
PubMed1983

Studies on experimentally induced hydrosalpinges in rabbits--experimental approach for tubo-microsurgery.

Hydrosalpinx proved macroscopically and microscopically which was quite similar to that observed in the damage human fallopian tube could be induced by ligation of the rabbit fallopian tube. Where ligatures have been placed on two places of the fallopian tube, hydrosalpinx can be induced in 100 percent of cases if ligation is done securely. About 1-2 weeks after ligation, no change of ciliated cells was observed in the mucosal fold in SEM findings, nor was there much change in the external shape of non-ciliated cells even though hydrosalpinx was induced. With the lapse of time after development of hydrosalpinx, however, both of these cells on the surface of the mucosal fold gradually changed to loose their normal shapes. After 15 weeks, ciliated cells all but disappeared to be replaced by large, filled, irregular non-ciliated cells. Accordingly, hydrosalpinx in the early stage can be cured fully by tubal salpingostomy using microsurgery. In the case of hydrosalpinx many hours after ligation, however, end-to-end anastomosis of the normal regions should be performed since no recovery can be expected.

Animals↗