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Biomedical subjects

M Neri

Publications and source records attributed to M Neri.

At least 145 records · Page 8Linked to original sources
PubMed1986

Natural resistance in mice against Friend leukemia cells. I. Studies with in vitro passaged interferon-sensitive and interferon-resistant cell clones.

Anti-lymphoma natural resistance (NR) has been detected in DBA/2 mice inoculated intravenously (iv) with syngeneic Friend leukemia cells (FLC). Interferon-sensitive 745 or interferon-resistant 3Cl-8 clones, passaged in vitro and exhibiting "low" tumorigenicity in syngeneic DBA/2 mice, were used. NR, measured as rapid clearance of radiolabeled cells from lung and liver of recipient mice, was age-dependent, was boosted by host pretreatment with polyinosinic-polycytidylic (poly I:C) acid or Friend leukemia virus, and was decreased by mice pretreatment with cyclophosphamide or i-carrageenan. Treatment of "target" FLC with interferon suppressed the susceptibility of 745 FLC, but not that of 3Cl-8 FLC to host's NR. These data suggest that the "low" in vivo tumorigenicity of in vitro passaged FLC is, at least in part, due to host's NR directed against target structures associated with leukemia cells.

Aging↗
PubMed1985

[Physiology of interdigestive motor activity in man].

Aim of this work has been to investigate the pattern of interdigestive motility from the esophagus to the duodenum. Six human subjects have been studied by means of a manometric probe. The results are very similar to those described elsewhere, and show that a cyclic motor activity spreads down from the esophagus to the duodenum and that a district control of gastrointestinal motility exists.

Adult↗
PubMed1985

The natural history of jumper's knee. Patellar or quadriceps tendonitis.

We have reviewed the clinical records and the results of conservative and surgical treatment of 125 out of 172 cases of Jumper's Knee followed for at least 2 years since the onset of symptoms. All were athletes involved in various sporting activities. At follow up 15 patients had given up sport and the lesion had healed spontaneously. We used the classification of Blazina et al as modified by Roels et al. Twenty-four patients were in stage 1, 42 in stage 2, 43 in stage 3 and one in stage 4. Conservative treatment was used primarily on all patients with one exception where the tendon had ruptured. The results were very good in 42, good in 32, and poor in 43, but in 36 patients a long period of rest and reduction in sporting activity was necessary before a significant improvement was obtained. Operation was carried out on 16 patients (19 knees), 18 were in stage 3 and one in stage 4. Various surgical techniques were used. The results were very good in 7 knees, good in 5 and poor in 7. Operation is indicated after the failure of conservative measures and in patients who do not want to reduce their sporting level. The aim should be to induce healing at the bone tendon junction. We emphasize the importance of the prevention of jumper's knee in athletes.

Athletic Injuries↗
PubMed1985

Pain measurements in right-left cerebral lesions.

Left- and right-brain damaged (BD) subjects were examined to ascertain whether psychophysical parameters of pain-pain threshold (P), tolerance threshold (T), and pain endurance (E)-were modified by brain damage and whether differences exist between LBD and RBD. Noxious stimulus was provided by electrical stimulation. Results showed that P and T scores for the paralysed arms were consistently higher than those of the contralateral side. This was not simply due to modified sensitivity alone; in the RBD group hemi-inattention and aphasia in the LBD group also played a role. A significantly hightened value of pain endurance was found only in the healthy arm in the RBD group.

Aged↗
PubMed1985

Atropine antagonizes cholecystokinin and cerulein-induced gallbladder evacuation in man: a real-time ultrasonographic study.

Dose-response curves for cholecystokinin (CCK), cerulein (CRL), and the effect of atropine on peptide-induced gallbladder evacuation, were evaluated in 13 normal subjects. Gallbladder volume was monitored by means of real-time ultrasonography. After an overnight fast, CCK was infused in six subjects iv, with increasing doses from 0.002 IDU/kg/min for 15 min, and CRL in seven subjects from 0.5 ng/kg/min for 5 min, until maximum gallbladder evacuation (greater than 70% of the fasting volume) was achieved. Forty-eight hours after the first study, CCK and CRL were readministered at the maximum contracting dose in each subject with and without a pretreatment with atropine (1 mg iv as bolus). The results showed maximum gallbladder evacuation at 0.016 IDU for CCK, and at 4 ng for CRL. Atropine significantly blunted the gallbladder response both to CCK and to CRL. It is therefore suggested that the cholinergic system is involved in the gallbladder response to CCK and to CRL.

Adolescent↗
PubMed1984

Natural resistance against hematopoietic cells in lethally-irradiated mice infected with Friend leukemia virus.

The influence of in vivo infection with the polycythemic substrain of Friend leukemia virus on noninducible ('natural') resistance against allogeneic normal or malignant grafts was studied in lethally irradiated mice. Parallel studies were performed on the NK system in the same experimental conditions. The results indicate that FLV-P infection of mice with full (DBA/2) vs partial (BALB/c and CD2F1) susceptibility did not suppress their in vivo natural resistance against bone marrow or El-4 leukemia cells. On the other hand, a decline in NK activity paralleled the progression of leukemic disease in the more susceptible DBA/2 hosts.

Animals↗
PubMed1984

Aging and right-left asymmetry in experimental pain measurement.

Verbal psychophysical measurements were performed on 100 subjects, both male and female, aged from 20 to 82 years, to ascertain whether different responses to pain exist between the right and left sides of the body in relation to aging and to different capacities of the cerebral hemispheres to process emotions. A single-phase step current applied to the forearm provided a standard noxious stimulus. Sensory threshold (S), pain threshold (P), and tolerance threshold (T) were measured. The sample set was divided into 2 comparable groups either older or younger than 60 years of age. A common trend was found in both groups: right-side scores were consistently lower than the homologous left-side scores. This difference was significant for all thresholds in elderly subjects but only for the pain threshold in the younger subject group. Two-way ANOVA test of the two group scores did not reveal differences due to sex, but age was an influencing factor for sensory and pain thresholds, higher scores being found in the older subject group. An interpretation of the results embodies hemispheric capacities to process verbal and emotional stimuli.

Adult↗
PubMed1984

Pain endurance in unilateral cerebral lesions.

The purpose of the present experiment was to study whether a unilateral cerebral lesion modifies pain parameters with respect to a control group. Electric stimuli were applied to brain damaged patients on the forearm ipsilateral to the lesion, and to both forearms of a control group. No significant difference was found between experimental groups for either pain or tolerance thresholds, whereas pain endurance--the difference between pain threshold and pain tolerance threshold--was significantly greater in right brain damaged patients, compared to both the control group and the left brain damaged group. In the latter group, pain endurance was not significantly different from controls, although there was a tendency towards its decrease. Result are discussed in relation to the problem of the hemispheric specialization for emotional processes.

Aged↗
PubMed1983

DNA-DNA hybridization assay for detection of Salmonella spp. in foods.

We have developed a DNA-DNA hybridization test for the presence of Salmonella spp. in foods. This test requires an initial pre-enrichment of food samples in nutrient broth but does not require selective enrichment. Samples of food cultures are collected on membrane filters and assayed by molecular hybridization to labeled probes. The probes consist of DNA sequences which are unique to the genus Salmonella and are widely distributed in the genus. A diverse panel of foods was assayed successfully by this methodology.

Bacteriological Techniques↗
PubMed1981

Combined effects of natural resistance and chemotherapy against a murine lymphoma in lethally-irradiated mice.

Lymphoma EL-4 of B6 origin was inoculated into lethally-irradiated syngeneic B6 or resistant BD2F1 hybrid recipients. Marked impairment of lymphoma cell proliferation (i.e. hybrid resistance, HR) occurred in the spleen but not in the liver or lung of BD2F1 hosts. Treatment with DTIC at the optimal dose of 80 mg/kg i.p. produced inhibitory effects in spleen, liver and lung of young BD2F1 mice, that were much greater than those observed in syngeneic B6 hosts. Increased anti-lymphoma effects for HR and DTIC chemotherapy were not detectable in the liver or lung of old BD2F1 mice or in the spleen, liver or lung of young hybrid recipients depressed for HR by pretreatment with cyclophosphamide. This is the first report on combined antileukemic effects of the host's anti-tumor natural resistance and chemotherapy.

Animals↗
PubMed1981

Inhibition of hybrid resistance to lymphomas by inactivated tumor cells in lethally irradiated mice.

Two murine lymphomas, L5MF-22 of B10.129(5M) (H-2b) origin and P388 of DBA/2 (H-2d) origin, were inoculated into lethally irradiated hybrid (C57BL/6 x DBA/2)F1 (B6D2F1) mice (H-2b/H-2d). Marked localized graft resistance was found in the spleen and occasionally in the liver of recipient mice as a result of a non-T-dependent hybrid resistance (HR). Significant reduction of HR of B6D2F1 mice could be obtained when viable lymphoma cells were inoculated along with inactivated cells of the same tumor or of genetically related leukemias. These results suggest that in vivo competition for HR effectors can take place in mouse spleen and liver leading to a depression of the localized resistance.

Animals↗
PubMed1980

[The first stage of sarcoidosis: the actual diagnostic possibilities (author's transl)].

A correct staging of sarcoidosis is of the uppermost importance in the diagnosis and treatment of the disease. In the last few years new concepts have been stated: --radiological staging of sarcoidosis is particularly difficult --in no one phase the radiological pattern can be considered typical but sometimes it is highly suggestive for sarcoidosis --anatomical studies recently showed that sarcoid disease seems to begin with a non granulomatous interstitial pneumonia. Following this individual microscopic deposit may conglomerate to form the typical non caseating granulomas. In the lung the disease seems to procede from the interstitium to the lymphnodes. Usually, in the first stage, possible sarcoidosis is diagnosed by the radiologist, but a confident diagnosis needs a pathological confirmation. Transbronchial biopsy guided by fluoroscopy is the first diagnostic choice for obtaining confirmation of the disease and for determining the type and entity of parenchymal lesions.

Adult↗