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Biomedical subjects

M Nakano

Publications and source records attributed to M Nakano.

At least 829 records · Page 46Linked to original sources
PubMed1990

[Two cases of supposed metastasizing leiomyosarcoma of the uterus].

Case 1. A 57-year-old female had hysterectomy 10 years ago because of leiomyoma uteri. She was recently found to have a clear-cut round mass shadow in the right middle lung field on her chest X-ray film 3. The mass was resected and microscopically diagnosed as metastatic leiomyosarcoma. Re-examination of the pathological specimen of the uterine tumor resected 10 years ago revealed 5-9 mitosis per 10 high-powered fields (/10 HPF). Case 2. A 54-year-old female who had undergone hysterectomy for myoma uteri 9 years previously was found to have a well-defined oval shadow in the left lower lung field which was resected and pathologically diagnosed as metastatic leiomyosarcoma. Her uterus lesion microscopically revealed 1-2 mitosis/10 HPF. The relationship between metastatic leiomyosarcoma of the lung and mitotic activity of leiomyoma uteri was discussed.

Female↗
PubMed1990

[Investigation of a patient with difficult endotracheal intubation].

Unusually difficult endotracheal intubation was encountered during anesthetic induction of a 41-year-old male, necessitating use of a bronchofiberscope. The usual pre-operative analysis had shown no problems, such as tracheal tumor or stricture. Due to difficult intubation, post-operative evaluations were made and no abnormalities were found. However, investigation of the lateral roentgenogram showed a much greater than average backward bend in the patient's trachea under the vocal cord. It is likely that the sharp bend of the trachea was responsible for difficult intubation.

Adult↗
PubMed1990

[Clinical application of cultured autologous epithelium to donor sites for split-thickness skin graft].

The present study investigated the interaction between the in situ dermis by using the donor site for split-thickness skin graft (STSG) and the cultured autologous epithelium in eleven individuals. Human epithelial cells were cultured according to the method of Rheinwald and Green, with some modification. The mirror-image site was covered by ointment dressing, and used as control. All of the grafted epithelial adhered to the wound bed within 7-8 days, and they were thick enough to be manipulated. On the other hand, the control areas didn't re-epithelize until 13-18th day postoperatively. Pain and exudate were remarkably reduced in all the cases, and there was less itching in 9 cases, as compared to the control areas (P less than 0.01; sign test). Even though there was no visual difference of scarring after 3 months, the grafted areas were much softer than the control areas in 8 cases (P less than 0.01). There was also a definite difference concerning pigmentation decrease in 5 cases. 7 days post-grafting, the cultured epithelium became thicker and more differentiated in 8-10 cell layers, and a well-developed basal lamina was observed by PAS stain and on electron micrographs. A 14th day specimen from the grafted area showed almost normal epidermis with melanin granules in the basal layer, despite of a mild intercellular edema among basal and spinous cells. Biopsies after 3 months revealed that there was less dermal fibrosis in the grafted areas than in the ungrafted ones. There appeared a tendency of rapid healing associated with minimal fibrosis, leading to satisfactory results. It is possible to infer that the epithelial-dermal interaction induced by cultured epithelial autograft may influence and regulate the formation of collagen and other extracellular matrix by fibroblasts. This study suggests that cultured autologous epithelium can provide a successful permanent skin substitutes in the donor site for STSG.

Adult↗
PubMed1990

[Two cases of ureteral tumors occurring after operation on bladder tumors].

Among 116 patients with bladder tumors treated during a 17-year period, 2 patients were found to have ureteral tumors (1.7%). The first case was found 8 years after transurethral resection (T.U.R.) and the second case was diagnosed as multiple ureteral tumors 3 years after six T.U.R.s. Long term follow-up by urinary cytology should be performed for patients with bladder tumors to find upper urinary tract tumors, especially for the patient with multiple bladder tumors.

Aged↗
PubMed1990

[A case of progressive systemic sclerosis associated with mutilans-type arthropathy and suspected Felty's syndrome].

A patient who developed mutilans-type arthropathy, splenomegaly, leukopenia, leg ulcer and massive hydroxyapatite accumulation during the course of progressive systemic sclerosis (PSS) was reported. A 56-years-old female had suffered Raynaud's phenomenon since the beginning of her third decade. She developed multiple symmetrical arthritis and morning stiffness at the age of 29, and was treated with NSAIDs and low dose corticosteroids under the diagnosis of rheumatoid arthritis (RA) Because of dysphagia and diarrhea, she was admitted in Niigata-Kenritsu Senami Hospital in September, 1987. Physical and roentgenographic examinations revealed diffuse scleroderma, mutilans-type arthropathy, lung fibrosis, splenomegaly and right leg ulcer. Laboratory examinations showed leukopenia, high titer of anti-DNA antibody, positive anti-Scl-70 antibody and mild hypocomplementemia. These findings suggested that she had PSS and Felty's syndrome. Furthermore, massive subcutaneous and intraarticular hydroxyapatite accumulation were noticed. The leg ulcer and laboratory data gradually improved with the combination therapy of corticosteroids, D-penicillamine and plasmapheresis. Although it has been well recognized that PSS patients reveal frequently the articular lesions similar to these of RA, severe mutilans-type arthropathy seen in this case is extremely rare. The joint contracture might be induced by hydroxyapatite accumulation, of which the early diagnosis seems to be very important in long-standing PSS patients.

Antibodies, Antinuclear↗
PubMed1990

[Dermal anesthesia: comparison of the analgesic effects of 2% and 10% lidocaine gel patch].

The analgesic effects of aqueous gel containing 2% or 10% lidocaine with 3% glycyrrhetinic acid mono 3-0 hemiphthalate sodium as an absorption promoter were compared in two volunteer groups of 12 persons each. A round sponge (25 mm in diameter and 1mm in thickness) filled with approximately 0.3g of either gel was applied on the volar surface of the forearm and kept covered with an adhesive plastic film (Tegaderm) for two hours. The analgesic effect was assessed every 30 min by pin-prick method at five places under the coverage for two hours, and after the gel was wiped away. The result from each place was scored 0 (no pain) or 1 (needle pain). The mean pain scores at 1 hr and 1.5 hr in the 10% group were 1.0 and 0.7, and significantly lower than 2.2 and 1.3 of the 2% group (P less than 0.05). Two hour application of the gel, five volunteers in the 2% group and eight volunteers in the 10% group produced a pain score under 1.0. In these subjects, a 26 gauge needle was stuck into the skin for further pain analysis. Four of the 5 subjects in the 2% group and 7 of the 8 subjects in the 10% group did not complain of any pain. Transient local redness under the coverage was observed in 3 subjects in each group. They were all known to be sensitive to alcoholic beverages. No other side effects were found. The plasma concentration of lidocaine was lower than 0.01 microgram.ml-1 at all times.

Adult↗
PubMed1990

[Mechanism of hyperferritinemia in a case of malignant histiocytosis].

We report a case of malignant histiocytosis diagnosed by liver-spleen biopsy under laparoscopy. A 49-year-old woman was admitted to our hospital with thrombocytopenia, moderate anemia and hypoproteinemia. Her bone marrow findings revealed erythroid and megakaryocyte hyperplasia, and the serum ferritin concentration was 2,250 ng/ml though she had not received any blood transfusions. Ferrokinetics analysis showed the pattern of ineffective erythropoiesis, and the half-lives of erythrocytes and platelets were both shortened. Her hepatosplenomegaly gradually increased accompanied by increasing serum ferritin level to 10,000 ng/ml. Liver-spleen biopsy was carried out under laparoscopy and revealed infiltration of atypical histiocytes with erythrophagocytosis, which were positive for S-100 and ferritin but negative for lysozyme. The rate of glycosylation in whole serum ferritin, analyzed by using concanavalin-A binding method, showed that her glycosylated ferritin content was only 8.3%, whereas in sera after iron overloading, it was about 70%. Serum isoferritin profiles by isoelectric focussing were studied, and isoferritin pattern from malignant histiocytosis was the same as that in iron overloading after neuraminidase treatment. These findings suggest that serum ferritin is synthesized in proliferating histiocytes and released in the plasma as a nonsecretory type (nonglycosylated ferritin) in this case.

Female↗
PubMed1990

[Production and secretion of BSF2/IL6 in a case of hairy cell leukemia with polyclonal hypergammaglobulinemia].

A 55-year-old woman was first seen in October 1986, because of splenomegaly, moderate anemia and leukocytosis. The hemoglobin was 8.8 g/dl, platelet count 24.4 X 10(4)/microliters, and the white cell count 23,800/microliters with 73% atypical lymphoid cells. The bone marrow nucleated cell count was 99,000/microliters with 36% lymphoid cells. These atypical lymphoid cells showed hairy appearance under phase-contrast microscopy, and were positive for tartrate-resistant acid phosphatase. These cells showed the surface phenotype of CD10, CD19, CD20, Leu M5, HCM, and IgG K. Biochemical data revealed marked polyclonal hypergammagloburinemia (PHG) of IgG type (IgG 8756 mg/dl). To elucidate the mechanism of the PHG, we investigated whether hairy cells produce interleukin 6 (IL-6) and express IL-6 receptor. The culture supernatant of these hairy cells increased 3H-thymidine uptake of a IL-6 dependent hybridoma clone (MH60) in a dose-dependent manner. These cells were stained with anti-IL-6 antibody using immuno-cytochemical technique. Our results suggested that these hairy cells produce and secrete IL-6. Immunocytochemical staining with anti IL-6 receptor antibody and the binding assay with 125I-labelled recombinant IL-6 revealed that these cells express little or no receptors for IL-6. It was therefore suggested that IL-6 produced by hairy cells in this case is not an autocrine growth factor for these cells but may play a role in development of PHG by stimulating normal B lymphocytes to produce an excessive amount of immunoglobulin.

B-Lymphocytes↗
PubMed1989

Intraarterial infusion of 5-fluoro-2-deoxyuridine-C8 dissolved in a lymphographic agent in malignant liver tumors. A preliminary report.

The drug 5-fluoro-2-deoxyuridine-C8 (FUdR-C8), one of the lipophilic prodrugs of FUdR, was dissolved in an oily lymphographic agent (Lipiodol Ultra Fluid, Andre Gelbe Laboratory, Paris, France; Ethiodol, Savage Laboratories, Melville, NY) and used for the intraarterial treatment of malignant liver tumors. From August 1985 to June 1988, 33 patients with hepatocellular carcinoma and 13 patients with metastatic liver tumors were treated with this agent at the Kumamoto University Hospital and its affiliated hospitals. The response rate (complete remission [CR] and partial remission [PR]) was 27.6% for hepatocellular carcinomas and 46.1% for metastatic liver tumors. The cumulative 1-year survival rate was 55.1% for hepatocellular carcinomas and 70.0% for metastatic liver tumors. More than a 50% decrease in the tumor marker level was observed in ten of 21 patients with hepatocellular carcinoma and in two of eight patients with metastatic liver tumors. The side effects, which were transient and controlled with conservative treatment, included fever, abdominal pain, nausea, vomiting, and acute gastritis.

Adult↗
PubMed1989

Steric course of the hydrolysis of alpha,alpha-trehalose and alpha-D-glucosyl fluoride catalyzed by pig kidney trehalase.

We are unable to confirm the report of Labat et al.3 that pig kidney trehalase hydrolyzes alpha,alpha-trehalose to form solely alpha-D-glucose. Highly purified trehalase from pig renal cortex was found, in reactions monitored by 1H-n.m.r. spectra, to hydrolyze alpha,alpha-trehalose with the formation of both alpha- and beta-D-glucose. That the beta anomer constitutes the enzymically mobilized glucosyl residue is indicated by the further finding that beta-D-glucose is the product formed on hydrolysis of alpha-D-glucosyl fluoride by the enzyme. Present results show the stereochemical behavior of pig kidney trehalase in hydrolyzing alpha,alpha-trehalose to be indistinguishable from that reported by ourselves and others for trehalase preparations from a range of biological sources including rabbit renal cortex.

Animals↗
PubMed1989

Hepatic arterial injection chemotherapy with cisplatin suspended in an oily lymphographic agent for hepatocellular carcinoma.

A method to prepare cisplatin suspended in an oily lymphographic agent, Lipiodol (LPS), has been established to deliver cisplatin to hepatocellular carcinoma (HCC) by the hepatic artery. Seventy-one patients, one Stage I, 16 Stage II, 16 Stage III, and 38 Stage IV, were treated with LPS therapy. A partial response was obtained in 33 cases (46.5%), a minor response in 20 cases (28.2%), and no change in 18 cases (25.3%). In 34 patients whose serum alpha-fetoprotein (AFP) levels were greater than 400 ng/ml, the serum AFP levels decreased in 31 patients (91.2%). The AFP decreased by more than 50% in 25 cases (73.5%) and more than 75% in 19 cases (55.9%). The plasma des-gamma-carboxy prothrombin (DCP) levels decreased in all of the 26 DCP-positive patients. The survival rate was 77% at 6 months and the 1-year survival rate was estimated to be 55%. The patients treated with LPS therapy survived longer compared with patients given Lipiodol containing neocarzinostatin by the hepatic artery. Complications such as acute gastroduodenal mucosal lesions (24%), cholecystitis (2.8%), pancreatitis (7%), delayed jaundice (7%), and hepatic encephalopathy (4.2%) were observed after therapy. The peak plasma platinum (Pt) concentrations determined as ultrafilterable Pt occurred 5 to 20 minutes, and 5 to 60 minutes as total Pt after the end of LPS injection. The Pt concentrations in the tumor tissues were 42 times higher in four operated cases and 7.1 times higher in six autopsy cases than those in the nontumorous tissue. These results suggest that LPS selectively accumulates in the HCC, is long-lasting and gradually releases the drug. In addition it is effective as a new anti-cancer therapy for hepatocellular carcinoma.

Adult↗
PubMed1989

A case of biliary cystadenocarcinoma with morphologic and histochemical features of hepatocytes.

A case of biliary cystadenocarcinoma is presented in which the lesion seemed to partially differentiate into hepatocellular carcinoma. This patient had a cystic tumor in the left lobe of the liver, the interior being filled with papillary tumors. Histologic examination disclosed papillary growth of columnar tumor cells, together with tumor cells akin to hepatocytes (they had a distinct cell boundary, nuclear membrane, and nucleolus) showing a cobblestone appearance in some areas. Electron microscopic examination of this area showed bile canalicular structures. Histochemically, the tissue stained positively with the Luna-Ishak canalicular (LIC) technique; periodic acid-Schiff (PAS) staining demonstrated coexisting glycogen granulo-positive cells and mucus-producing cells. Thus, in this biliary cystadenocarcinoma, some tumor cells demonstrated the morphologic and histochemical features of hepatocytes.

Aged↗
PubMed1989

Rapid and sensitive determination of aprindine in serum by gas chromatography using a surface ionization detector.

A rapid and highly sensitive method for the determination in serum of aprindine, an antiarrhythmic drug, was developed employing gas chromatography with a surface ionization detector. No interfering peak from endogenous substances appeared when an organic phase was directly injected into the system after single extraction from a serum sample. A standard curve obtained was linear up to the serum level of 6 micrograms/ml, and the limit of sensitivity was 16 pg. The method described is applicable to routine therapeutic monitoring of serum concentrations of aprindine.

Adolescent↗
PubMed1989

A novel nonpeptidic, orally active renin inhibitor.

BW-175 is a newly synthesized renin inhibitor which is a nonpeptidic, norleucine analog. Its IC50 values for renin activity in human, squirrel monkey, marmoset, dog, hog, rabbit and rat plasma were 3.3, 6.6, 2.4, 42, 110, 86 and 3500 nM, respectively, and 26 microM for cathepsin D. Pepsin and angiotensin converting enzyme were hardly inhibited at 10(-4) M. BW-175 showed an oral bioavailability of 2.8% at 10 mg/kg and 9.7% at 30 mg/kg in rats. In normotensive, furosemide-treated high-renin marmosets, BW-175 (30 mg/kg p.o.) caused an intensive reduction in plasma renin activity and plasma angiotensin I formation, associated with a reduction in systolic blood pressure of 10-20 mm Hg for 2 hours.

Aminocaproates↗
PubMed1989

Generation of O2- and tyrosine cation-mediated chemiluminescence during the fertilization of sea urchin eggs.

Sea urchin eggs emit light in the visible region during their fertilization. Judging from the chemiluminescence spectra, one of the excited species generated is considered to have originated from a tyrosine cation radical-mediated reaction. Chemiluminescence probes such as luminol or a cypridina luciferin analog (See text) are useful for detecting the ovoperoxidase +H2O2 reaction associated with membrane hardening and O2- generation, respectively, during fertilization of sea urchin eggs.

Animals↗
PubMed1989

Defect of calmodulin-binding protein in expression of interleukin-1 beta gene by LPS-nonresponder C3H/HeJ mouse macrophages.

Peritoneal macrophages of Lipopolysaccharide (LPS)-refractory C3H/HeJ mouse failed to express the mRNA coding interleukin 1 (IL-1) beta when stimulated by the Ca2+ ionophore A23187 or LPS, though macrophages of LPS-responsive C3H/He responded to these stimulants. These results suggest that the defect of the response in C3H/HeJ macrophages toward LPS stimulation may be related to the Ca2+-dependent signal pathway. The extracts from the C3H/HeJ macrophages showed normal activities of both protein kinase C (PKC) and calmodulin (CaM) in comparison with those from LPS-responsive C3H/He macrophages. However, one species of CaM-binding proteins could hardly be detected by the cross-linking assay with 125I-CaM in C3H/HeJ macrophages stimulated by LPS. These results suggest that the LPS-refractory site in C3H/HeJ macrophages is related to the lack of this CaM-binding protein, and the Ca2+-dependent CaM system may play an important role in the activation of cells by LPS.

Animals↗
PubMed1989

Protein intake and renal function in children.

To evaluate the effects of protein intake on renal tubular functions, seven metabolic studies were performed on five adolescents with mild, chronic renal insufficiency. After equilibration, patients were randomly assigned to two periods of four days each on either a high-protein diet (HPD) or a low-protein diet (LPD), with energy intakes remaining the same during both periods. High protein intake resulted in significantly higher mean (+/- SD) daily creatinine excretion and clearance rates. The HPD was associated with significantly increased excretion rates of potassium, phosphate, sulfate, and calcium. Net acid excretion with the HPD was nearly threefold higher than that observed with the LPD. These differences were unassociated with urinary pH, which remained similar with both diets. We conclude that different protein intakes affect urinary excretion of creatinine, potassium, phosphate, sulfate, calcium, and net acid.

Adolescent↗