[Re-recurrence of left atrial myxoma in the right atrium].
Explore the source record for details and available documents.
Adult↗
Biomedical subjects
M Nakai
Publications and source records attributed to M Nakai.
Systemic and regional hemodynamic effects of E614 (tripamide) as studied in conscious SHR.
Experiments were conducted on 53 conscious young male spontaneously hypertensive rats utilizing radiolabeled microspheres to analyze the acute and chronic hemodynamic effects of a newly synthesized antihypertensive drug, E614. The hemodynamic variables were observed on the 1st and 6th days of E614 treatment (p.o., once daily) in separate groups of rats. When the drug was given at a smaller dose (10 mg/kg body weight) over 6 days, the hypotensive action of E614 was found to be due mainly to a reduction of the total peripheral vascular resistance with no significant change in the cardiac output (CO). A supra-pharmacological dose (100 mg/kg), however, lowered the mean arterial pressure (AP) due mainly to a significant reduction of the CO in both the acute and chronic treatments. We found little influence of the drug on heart rate, and the coronary and renal vascular beds in any of the present experimental settings. The cerebral blood flow appeared not to change significantly unless the AP fell to such an extent that a fall in cerebral blood flow took place despite the autoregulatory mechanism of the brain blood vessels. The tissue perfusion was also well preserved in the gastrointestinal tract.
Animals↗
Lectin-binding patterns in the developing tooth.
The lectin-binding patterns of the cells involved in amelogenesis and dentinogenesis in developing teeth of rats were studied. Undifferentiated odontogenic epithelia exhibited very slight staining with almost all of the lectins examined. The lectin-staining affinities of secretory ameloblasts could be divided into two categories: Concanavalin-A (Con-A), Wheat germ agglutinin (WGA) and Soybean agglutinin (SBA) binding occurred from the middle to apical cytoplasm, whereas Ricinus communis agglutinin-I (RCA-I) and Ulex europeus I (UEA-I) binding predominated in the basal regions. The cells of the stratum intermedium exhibited relatively strange lectin staining, which appeared to be dependent on ameloblastic maturation. The basement membranes in undifferentiated epithelia were markedly positive for lectin binding. Odontoblasts showed moderate Con-A staining on the apical side of the cells, as well as slight-to-moderate reactions with WGA and SBA. Pulp cells and dental papillae showed slight-to-moderate lectin staining, and predentin and dentin were also moderately positive for Con-A and RCA-I binding and slightly so for WGA and SBA. The lectin-binding affinities were enhanced during the formation of enamel and dentin, and appeared to be dependent on the degree of cellular differentiation in ameloblasts and odontoblasts.
Ameloblasts↗
Entry of adult T-cell leukemia-associated virus into human peripheral blood leukocytes. Brief report.
Within 10 minutes after tritium-labeled adult T-cell leukemia-associated virus (ATLV) inoculation, silver grains were found over human lymphocytes. At the time of entry of ATLV, the viral envelope was observed to fuse with the cell membrane.
Adult↗
Evaluation of phasic blood flow velocity in the great cardiac vein by a laser Doppler method.
In the present study, characteristics of the phasic flow pattern in the great cardiac vein and the mechanism of such pattern formation were investigated using a laser Doppler velocimeter with an optic fiber probe. The laser Doppler velocimeter allowed measurements of venous blood velocity under more physiological conditions than were possible with previous methods. Moreover, venous blood flow measurement in the great cardiac vein mirrors the effects of myocardial contraction on the venous flow more directly than does measurement in the coronary sinus. Thus, our method is considered very useful. Results obtained from the present study are as follows: 1) Measurement of the phasic flow in the great cardiac vein was made in 11 anesthetized dogs using our laser Doppler method. The blood velocity curve obtained in the great cardiac vein was always characterized by a prominent systolic flow wave (SFW). The mean value for the maximum velocities under control conditions in 11 cases was 40 +/- 13 cm/s. The blood velocity increased with the onset of left ventricular ejection and decreased gradually after the peak formation at mid- or late systole.--2) Besides the above SFW, one or two small wave components were frequently observed during the atrial contraction period and/or during the isovolumic contraction phase. On the waveform during the atrial contraction period, two cases showed forward flow, while one case showed reverse flow. The small reverse flow waves during the isovolumic contraction phase were found in seven cases.(ABSTRACT TRUNCATED AT 250 WORDS)
Animals↗
Detection of a pregnancy-associated protein.
Monospecific antiserum was produced with the use of a placental extract showing high activity of a proteinase which hydrolyses a synthetic substrate, N-benzoyl-D,L-arginine p-nitroanilide, used for measuring tryptic activity. An immunological study showed that antibodies were not generated against the component with this activity but against an antigen which was not related to several well-known pregnancy-associated proteins. Pregnancy sera contained an antigen which immunologically was completely identical to the antigen of placental origin. Single radial immunodiffusion showed an elevated level of reactive antigen in pregnant subjects: 70% of 333 cases were positive. Cross-reacting antigen was also detected in sera as well as ascitic fluid from cases of advanced ovarian cancer. Sephadex G-200 gel filtration indicated that the antigen has an apparent molecular weight of 94000, and immunoelectrophoresis showed the molecule to be of beta-mobility. These properties suggest that the substance may represent an additional pregnancy-associated protein entity. Partial purification and some immunological properties of this antigen are described.
Antigens↗
Distribution of keratin proteins in neoplastic and tumorlike lesions of squamous epithelium. An immunohistochemical study.
Seventy-six cases of tumorlike and neoplastic lesions from epidermis and oral epithelium were analyzed by a histochemical technique for the demonstration of keratin. Formalin-fixed paraffin sections were reacted with rabbit antihuman keratin antiserum (dilution of 1:40). The types of distribution of keratin in cells of lesions were classified into five categories: (1) regional, as found in normal squamous epithelia and benign hyperkeratinized lesions, and papilloma, and keratinized squamous cell carcinoma; (2) total, as seen in intensely keratinized lesions, such as verruca vulgaris and highly keratinized squamous cell carcinoma; (3) negative, as displayed by basal cell carcinoma; (4) scattered, as in the most poorly differentiated squamous cell carcinomas; and (5) mixed cellular, as found in both poorly and moderately differentiated squamous cell carcinomas.
Basal Cell Carcinoma↗
Histochemical studies of obstructive adenitis in human submandibular salivary glands. I. Immunohistochemical demonstration of lactoferrin, lysozyme and carcinoembryonic antigen.
Immunohistochemical detection of lactoferrin (LF), lysozyme (LZ) and carcinoembryonic antigen (CEA) was made in obstructive adenitis of the submandibular glands. Atrophic and altered acinar cells in the early stage of the lesion stained strongly for LF, whereas they were unreactive or stained slightly for LZ. Ductal cells usually stained for LZ. Staining for CEA was strong and irregularly distributed in altered acinar cells. Duct-like structures and dilated ductal segments in the chronic stage were generally negative for LF, LZ and CEA. Secretory components in luminal cavities gave abundant staining for LF, LZ and CEA. Histocytes which infiltrated into the connective tissue in the later stage showed a positive LZ reaction.
Acute Disease↗
Histochemical studies of obstructive adenitis in human submandibular salivary glands. II. Lectin binding and keratin distribution in the lesions.
Lectin-binding profiles and keratin distribution in obstructive adenitis of human submandibular glands (SMGs) are reported and compared those of normal SMGs. Histologically, obstructive changes in the SMGs included acinar atrophy, duct-like structure formation in the early stage, and disappearance of acinar cells and dilation of ductal segments in the later, chronic stage. The following lectins were used: Con A (Glc, Man), PNA(Gal, GalNAc), RCA-I(Gal), DBA(GalNAc), SBA(Gal, Gal-NAc), UEA-I(alpha-L-Fuc) and WGA(GlcNAC, NeuNAc). Lectin staining in atrophic acinar cells was usually reduced except for SBA binding and was irregularly distributed in altered acinar and ductal epithelia. Binding of DBA and UEA-I lectins were particularly intense along the luminar borders of ductal segments in the lesions. Immunohistochemically detectable keratins were characterized by intense staining in atrophic acinar cells and in all the ductal segments, whereas normal acinar cells, either serous or mucous, were negative.
Chronic Disease↗
An increase in cerebral blood flow elicited by electrical stimulation of the solitary nucleus in rats with cervical cordotomy and vagotomy.
The effect of monopolar electrical stimulation of the intermediate portion of the solitary nucleus (SOL) on brain circulation was investigated in 48 anesthetized (N2O), artificially ventilated Wistar rats. The cervical spinal cord and bilateral vago-sympathetic trunks were severed to eliminate any change in arterial blood pressure mediated by the autonomic nervous systems on stimulating the SOL. Blood pressure was kept within the physiological range by intravenous phenylephrine infusion. Current intensity of the stimulus was set at 60 +/- 4 microA (mean +/- S.E.; n = 9), which was equivalent to 2.5 times threshold for vasopressin-induced pressor response. A sufficient dose of vasopressin-antagonist given to each rat shortly before determination of cerebral blood flow, prevented vasopressin-induced hypertension from stimulating the medulla. Stimulation elicited an increase in regional blood flow (14C-iodoantipyrine technique) by 72%, and 39% on average in the frontal and occipital cortices, respectively, and 25% in the caudate-putamen (p less than 0.05; n = 12 in control, and 9 in the stimulation studies). It was found that observed results included little detectable contribution from current spread of the stimulus toward the adjacent structures (dorsal medullary reticular formation and cuneate fasciculus) surrounding the SOL and from retrograde activation of the cerebellar fastigial nucleus. Overall, the present study suggests that a certain vasomotor mechanism, which is activated upon excitation of the intermediate portion of the SOL, regulates the blood vessels of the brain as a vasodilator.
Animals↗
Approach for quantifying the overall function of the ascending central neural integration to release vasopressin in rats.
Electrical stimulation of the intermediate portion of the solitary nucleus (SOL) is known to consistently elicit a pressor response and increase in the release of vasopressin (VP) in rats, when the cervical spinal cord and vago-sympathetic trunks are cut. The aim of the present study was to provide a methodological assessment for this technique, in order to quantify the central neuroendocrine function to release VP. Experiments were conducted on rats with cervical cordotomy and vagotomy, under 60% N2O anesthesia and artificial ventilation. The animals were maintained at their normal arterial blood pressure by infusing phenylephrine, which itself exerted little influence on the arterial plasma VP concentration (pVP) during SOL stimulation. In order to prevent blood loss, cross circulation with a donor rat was utilized in the blood sampling for pVP determination. We confirmed that this technique itself did not significantly affect the pVP level. A monopolar stimulus at a frequency of 50 Hz was found to produce the pressor response most effectively. The current spread of the stimulation (0.5 ms duration; 1 s on/1 s off; up to at least 120 microA) did not induce a false-positive pressor response mediated by other structures adjacent to the SOL. We suggest that electrical stimulation of the SOL in rats with cervical cordotomy and vagotomy, for observing the pressor response and/or increase of pVP, represents a rapid approach for screening the overall function of the ascending efferent part of the reflex mechanisms to release VP.
Animals↗
Effects of aspartame on diabetic rats and diabetic patients.
The effects of aspartame (L-aspartyl-L-phenylalanine methyl ester) on plasma glucose and insulin levels were investigated in diabetic rats and patients with non-insulin-dependent diabetes mellitus. The oral administration of 0.45 mg aspartame per 100g body weight, which is equivalent to 150 mg of glucose in sweetness, to streptozotocin-induced diabetic rats had no effect on the plasma glucose or insulin levels. Also, 225 mg oral aspartame loading, which is equivalent to 75 g of glucose in sweetness, to patients with non-insulin-dependent diabetes mellitus did not increase plasma glucose or insulin levels, although 75 g of oral glucose loading increased plasma glucose and insulin levels in diabetic patients as expected. Aspartame ingestion for three days at a dose of 24-48 mg per day and the intake of snacks flavored with 240 mg of aspartame also did not increase fasting plasma glucose levels. These results suggest that acute administration of aspartame has no influence on plasma glucose or insulin levels in diabetic rats and patients with non-insulin-dependent diabetes mellitus.
Adult↗
[A patient with immunoblastic lymphadenopathy: possible relation to Epstein-Barr virus infection].
Explore the source record for details and available documents.
Aged↗
[A case of simultaneous operation of aorto-coronary bypass graft and lung cancer].
Explore the source record for details and available documents.
Adenocarcinoma↗
[Nonresective operations for thoracic or thoracoabdominal aortic aneurysms].
Explore the source record for details and available documents.
Acute Kidney Injury↗
Biological significances in granular cells of ameloblastoma--histochemical identification of sugar residues and filamentous proteins, morphometry and DNA cytometry of granular cells.
Explore the source record for details and available documents.
Ameloblastoma↗
Release of vasopressin by electrical stimulation of the intermediate portion of the nucleus of the tractus solitarius in rats with cervical spinal cordotomy and vagotomy.
The cervical spinal cord and vagi were severed in anesthetized and artificially ventilated rats. Monopolar electrical stimulation of the intermediate portion of the nucleus of the tractus solitarius (NTS) resulted in subtle increases in concentration of plasma vasopressin (pVP) and in arterial pressure. We suggest that electrical stimulation of the NTS in rats undergoing such surgical preparation to observe the pressor response and/or increase in pVP, represents a rapid approach for screening the neurosecretory function of the central neural integration to release vasopressin.
Animals↗
Monocular movement of contralateral eye following cortical stimulation in the coronal sulcus of cat.
Monocular movements of the contralateral eye were demonstrated following the stimulation of the internal kneelike portion of the cortex in the coronal sulcus of the cat. The stimulation of this cortical area in the coronal sulcus yielded rapid medial or slightly oblique ventromedial movement of the contralateral eye. While ablation of the same cortical area produced contralateral eye shift toward the dorsolateral side and the shifted eye became uncontrollable. The results suggested that the control mechanism of eye movement involves unilateral innervation system to the contralateral side as seen in the extremities.
Animals↗