Search PubMed⌕ Search

Biomedical subjects

M Nagano

Publications and source records attributed to M Nagano.

At least 361 records · Page 20Linked to original sources
PubMed1976

A new semisynthetic 7alpha-methoxycephalosporin, CS-1170: 7beta-((cyanomethyl)thio)acetamido)-7alpha-methoxy-3-((1-methyl-1H-tetrazol-5-yl)thio)methyl)-3-cephem-4-carboxylic acid.

The synthesis and antimicrobial activity of a new semisynthetic 7alpha-methoxycephalosporin,7beta [[(cyanomethyl)thiol]acetamidol]-7alpha-methoxy-3-[[(1-methyl-1H-tetrazol-5-yl)thio]-methyl]-3-cephem-4-carboxylic acid (CS-1170), are described. This compound shows interesting antibacterial activity when compared to cefoxitin and cephalothin.

Bacteria↗
PubMed1975

[Method for the isolation of human glomerular basement membrane (author's transl)].

In order to obtain pure glomerular basement membrane for chemical analysis, we could develop the following method. The isolated glomeruli are treated with a 0.08% polyoxyethylene-sorbitaine-monooleate for 3-5 minutes and they are homogenized in this detergent. The homogenate obtained is placed in a sucrose gradient with the specific density between 1021 to 1081. After standing for 30 minutes in a refrigator, the homogenate was separated into three layers. The uppermost layer contained fragmented cell debris, the second layer was consisted of the endothelial and epithelial cells. In the third layer, which was clearly separated from upper two layers by the translucent zone, the basement membrane were found. The position of the third layer is different in normal and pathological glomeruli, and the width of the layer is very narrow. According to this method only a very little contamination with other glomerular cell fraction is observed. The application of the 0.08% polyoxyethylene-sorbitane-monooleate did not induce any appreciable change in the structure of basement membrane, because carbohydrate composition and sialic acid content of this preparation were essentially identical with the data reported by several authors who prepared basement membrane by ultrasonic method. Electron microscope also revealed that the isolated glomerular basement membrane preserved lamina densa completely attached with partly stripped lamina rara interna and externa. Our method to break glomeruli is inexpensive and less time-consuming than the breakage with ultrasonic vibration. Isolation procudure with density gradient is obviously superior to the simple centrifugation or settling method adopted by others.

Basement Membrane↗
PubMed1975

Oxidative phosphorylation in mitochondria isolated from stressed rat heart.

Oxidative phosphorylation was measured polarographically in mitochondria isolated from rat heart. With regard to ADP/O ratio and respiratory control index, no differences were found among mitochondria isolated from normal, acutely (increased pneumatic resistance of the heart-lung preparation) and chronicly (renal hypertension) stressed heart. However, the acute stressed heart induced by strangulating the outflow tract of the heart-lung preparation showed clear depression and tendency to depression, respectively, in the level of ADP/O ratio and respiratory control index in mitochondria. The results indicate that there is no change in the oxidative phosphorylating mechanism of the myocardial mitochondria of nonfailed heart despite acute or chronic pressure loadings; however, there is an uncoupling of oxidative phosphorylation in mitochondria of the failed heart after acute strangulation of the outflow tract.

Adenosine Diphosphate↗