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Biomedical subjects

M Nachman

Publications and source records attributed to M Nachman.

22 records · Page 2Linked to original sources
PubMedDate not supplied

Affinity purification and characterization of anti-Tac(Fv)-C3-PE38KDEL: A highly potent cytotoxic agent specific to cells bearing IL-2 receptors.

A chimeric, single chain antibody fused immunotoxin, denoted anti-Tac(Fv)-C3-PE38KDEL, was engineered and expressed in Escherichia coli. The microbially expressed anti-Tac(Fv)-C3-PE38KDEL was solubilized from inclusion bodies using guanidine hydrochloride, and subsequently refolded in a redox buffer via thiol/disulfide exchange. The recombinant immunotoxin from the crude extract was purified employing receptor-affinity chromatography, which is based upon biological function and involved the immobilized p55 subunit of human IL-2 receptor. The cytotoxic activity of this immunotoxin was measured by the IL-2 dependent phytohemagglutinin (PHA) blast proliferation inhibition and HUT-102 protein synthesis inhibition assays, in which the IC50 values were 41.5 and 0.8 pM, respectively. The biochemical homogeneity and authenticity of the purified material were determined by gel permeation chromatography, amino acid composition and N-terminal sequence analyses, SDS-PAGE, isoelectric focusing, and Western blotting. The receptor-affinity-purified immunotoxin was shown to be highly effective in specifically killing cells bearing IL-2 receptors. Anti-Tac(Fv)-C3-PE38KDEL is a powerful immunosuppressant which may be a potentially useful therapeutic agent in the prevention of allograft rejection and in the treatment of autoimmune diseases. Another anticipated application of this fusion protein is as a chemotoxin in the treatment of some forms of cancer.

ADP Ribose Transferases↗
PubMedDate not supplied

[Vascular cerebral malformations in an adult with internal hydrocephalus and great cerebral vein varix undiagnosed in childhood].

Our material consists of the analysis of the results obtained during the postmortem angiologic research of the brain in a deceased 36-years-old woman treated in Neurosurgical Department of Medical university in Lódz. The patient was diagnosed as follows: Arteriovenous malformation (AVM) in the central region of the brain with giant "aneurysm" of the vein of Gallen. Internal hydrocephalus. The anatomical and angiological investigations were concerned mainly with the vessels of the Willis circle, choroid arteries and their branches both in subarachnoid space and in brain parenchyma--and those regions of the brain where pathological changes were detected angiologically. The reason for the comparative evaluation of the vascular changes was the material prepared earlier in the laboratory and literature data concerning: vascularization od choroid plexus of lateral and III ventricles of the brain and vascular deformities in brains of deceased adult and senile patients. This paper is also an attempt at comparing of the vascular changes in postmortem injection examination to results of radiological studies obtained in living patient (angiography of the internal carotid and vertebral arteries) and CT examination.

Adult↗