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M N Sotto

Publications and source records attributed to M N Sotto.

36 records · Page 2Linked to original sources
PubMed1990

Experimental visceral leishmaniasis: sequential events of granuloma formation at subcutaneous inoculation site.

Hamsters were inoculated with IO7 Leishmania (Leishmania) chagasi amastigotes in the hind footpads and killed at 7, 15, 30, 45, 60, 75 and 90 days after infection. We observed mononuclear inflammatory infiltrates with many parasites on the 7th and 15th days of infection. On the 30th day there was early granuloma formation. After 45 days the lesion was characterized by well defined epithelioid granuloma with multinuclear giant cells whose cytoplasm showed Schaumann bodies. Non-particulate antigenic material was present in the macrophage cytoplasm and between the lamellae of the Schaumann bodies. Granuloma formation has an important role for the control of infection at the inoculation site. The results indicate that dissemination of the infection must occur in the first 45 days, before granuloma formation has taken place.

Animals↗
PubMed1990

A case of multiple lesion mucocutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis infection.

A case of leishmaniasis with multiple lesions due to Leishmania (Viannia) braziliensis infections was diagnosed in a male patient from north-east Brazil. The skin lesions were nodular and pustular and progressed to ulcers. Skin and the oral mucosae were also affected. The behaviour in culture and hamsters of the parasite recovered from a skin lesion was similar to other strains of L. (V.) braziliensis and the strain was characterized by monoclonal antibodies as belonging to serodeme I of this species. The histopathology of the skin lesions of the patient and hamster were of an inflammatory nature which extended through the dermis; leishmania in the tissues were more easily detected by the immunoenzymatic PAP method.

Adult↗
PubMed1989

Cutaneous leishmaniasis of the New World: diagnostic immunopathology and antigen pathways in skin and mucosa.

Non-specific chronic inflammation and/or granulomatous reaction are the main histopathological manifestations of cutaneous and mucocutaneous leishmaniasis of the New World. Plasma cell infiltration associated with collagen and vascular changes are data suggestive but not diagnostic of the disease. Specific diagnosis is only possible through demonstration of the parasite in the tissue examined. It is noteworthy that the parasites are usually scanty and difficult to demonstrate in the lesions. Biopsies from 40 patients with cutaneous or mucocutaneous leishmaniasis were examined using the immunofluorescence and immunoperoxidase techniques in order to demonstrate the parasite and/or antigen in the tissues. Nineteen biopsies showed non-specific chronic inflammation and 21 a granulomatous reaction. Parasites were found in 20% of the routine biopsies. The positivity through indirect immunofluorescence was 88.46% in frozen sections of fresh material and 89.28% in paraffin embedded tissue. The antigen positivity with the immunoperoxidase technique was 64.51%. Antigen was detected as amastigotes and also as diffuse material in the macrophage cytoplasm and adsorbed in the epithelial basement membrane and vessel walls. There was no difference in the positivity of antigen according to the type of inflammatory reaction.

Animals↗
PubMed1988

Childhood Degos disease with prominent neurological symptoms: report of a clinicopathological case.

Degos disease is a rare disorder, characterized by a vasculopathy of unknown origin that leads to typical skin lesions and involves other organ systems. It is frequently a lethal condition; death occurs as a consequence of intestinal perforation. In about 20% of cases, the central nervous system is involved and the neurological symptoms can be prominent. The incidence of the disease in children is very uncommon. We report the case of teenage girl who had Degos disease with prominent neurological involvement.

Adolescent↗
PubMed1987

Salivary duct carcinoma: ultrastructural and histogenetic considerations.

Two cases of salivary duct carcinoma were examined by light and electron microscopy. Histologically, the tumor presented cribriform and papillary patterns together with comedonecrosis. Electron microscopy revealed duct cells with microvilli, interdigitations, and apical vesicles. No myoepithelial cells were observed. These findings suggest a ductal origin other than from the intercalated duct or its precursor element. The importance of separating the salivary gland adenocarcinomas is discussed.

Adenocarcinoma↗
PubMed1985

[Acquired epidermolysis bullosa (acquired dermolytic bullous dermatoses) (study of 2 patients)].

The authors studied two patients with bullous lesions with onset at adult age. There were no relatives with bullous disease. After minor traumas, mainly in hands, feet, elbows and knees, one could see the onset of the blisters, which vanished leaving atrophic scars. Nails and mucosae were involved in both patients. The female patient had cyst of milia. One of the patients had lymph node tuberculosis as associated disease. Through exhaustive clinical examination and laboratory investigation, the authors excluded other types of bullous diseases. Histopathology of the lesions, electron microscopy, direct and indirect immunofluorescence, supported the diagnosis of Acquired Dermolytic Bullous Dermatosis.

Adult↗
PubMed1984

[Post-mortem evaluation in endemic pemphigus foliaceus and pemphigus vulgaris].

Eighteen autopsies were performed in patients with pemphigus that died during treatment between 1961 and 1981. Eight of these patients had endemic pemphigus foliaceus and ten had pemphigus vulgaris. The pemphigus vulgaris patients were receiving only corticosteroids while some of those with pemphigus vulgaris also received immunosuppressors. Side effects from the medication were a major contributing factor in the death of 7 of the 8 pemphigus foliaceus patients and in 8 of the 10 cases of pemphigus vulgaris. The causa mortis to which the medication contributed to was strongyloidiasis in 4 patients, disseminated septicemia in 3 patients, bronchial pneumonia in 3 patients, miliary tuberculosis in 2 patients, massive gastrointestinal hemorrhage, pulmonary infarct and balantidiasis with strongyloidiasis in 1 patient each. The authors stress the need for careful investigation of strongyloidiasis and eradication by treatment not only when starting but also during therapy with large doses of corticosteroids and cytotoxic agents.

Adolescent↗
PubMed1980

South American pemphigus foliaceus: electron microscopy and immunoelectron localization of bound immunoglobulin in the skin and oral mucosa.

Five skin and two oral biopsies from patients with South American pemphigus foliaceus (SAPF) were studied by electron and immunoelectron microscopy for the ultrastructural localization of bound immunoglobulin in epidermal and oral lesions. Electron microscopy showed the tonofilament-desmosome complex to be preserved in the various layers of the epidermis. Immunoglobulin was bound over the plasma membrane and permeated the desmosomal junctions both in the skin and oral mucosa, thus suggesting that pemphigus antibodies are attached to the glycocalyx. It appears that the initial injury in SAPF acantholysis involves the glycocalyx and that it might be caused by interaction with intercellular antibodies present in the patient's serum.

Adult↗
PubMed1976

Pathogenesis of cutaneous lesions in acute meningococcemia in humans: light, immunofluorescent, and electron microscopic studies of skin biopsy specimens.

Biopsy specimens of the skin were taken from 10 patients with acute meningococcemia who exhibited mainly maculopurpuric lesions. The specimens were studied by light, electron, and immunofluorescent microscopy in an attempt to obtain information on the pathogenesis of vascular injury. Light microscopy disclosed a large number of Neisseria meningitidis organisms, both in the endothelial cells and being phagocytized by neutrophils. Vascular injury was characterized (by means of both light and electron microscopy) by endothelial necrosis, thrombosis, and necrosis of other elements of the vascular wall, such as muscle cells and pericytes. Immunoglobulins and complement were also found in the vascular wall in most cases. Hypercoagulability was demonstrated in some patients. These findings suggest that the cutaneous lesions of meningococcemia fulfill most of the gross and histologic criteria of the local Shwartzman reaction, but that immunological factors probably contribute to pathogenesis.

Blood Coagulation Tests↗
PubMedDate not supplied

Folliculitis ulerythematosa reticulata (atrophoderma vermiculata): early detection of a case with unilateral lesions.

A 5-year-old girl had a unilateral rash on her right cheek. Histologic investigations showed aberrant pilosebaceous units and elastotic material in the papillary dermis. Unilateral folliculitis ulerythematosa reticulata (a variant of keratosis pilaris atrophicans) is strikingly rare, and its very early clinical and microscopic changes are demonstrated in this case report.

Administration, Topical↗
PubMedDate not supplied

The ultrastructural and histophotometric study of elastic and collagen fibers in type II Ehlers-Danlos syndrome and subclinical forms.

Skin disorders in type II Ehlers-Danlos Syndrome (EDS) are characterized by signs of cutaneous hyperdistensibility, skin and vascular fragility, atrophic scars, and articular hypermobility. These features may have less important clinical presentation in the intermediate forms of type II EDS. The authors studied the ultrastructural and quantitative aspects of elastic and collagen fibers in the skin of individuals with subclinical signs of type II of Ehlers-Danlos Syndrome. A group of 27 individuals (Group I) with large atrophic scars, articular hypermobility of the hands, and cutaneous and vascular fragility were compared with 10 healthy individuals. The subjects from both groups were volunteers from Hospital das Clínicas da Universidade de São Paulo. The elastic fibers did not show alterations but collagen ultrastructural abnormalities were seen in diameter and curvature, such as torsion, collagen flower-like aspect and discrete mass enlargement by histophotometry.

Collagen↗
PubMedDate not supplied

Pattern of mycobacterial antigen detection in leprosy.

Immunohistochemistry reaction (Peroxidase anti-peroxidase--PAP) was carried out on fifty-two skin biopsies from leprosy patients with the purpose to identify the antigenic pattern in mycobacteria and to study the sensitivity of this method. Five different patterns were found: bacillar, granular, vesicular, cytoplasmatic and deposits, classified according to the antigenic material characteristics. Deposits (thinely particulate material) appeared more frequently, confirming the immunohistochemistry sensitivity to detect small amounts of antigens even when this material is not detected by histochemical stainings.

Antigens, Bacterial↗
PubMedDate not supplied

Persistence of Leishmania antigen in C57Bl/6j inbred mice infected with Leishmania (Leishmania) amazonensis.

PURPOSE: To develop an animal model for studying mucocutaneous leishmaniasis. METHODS: The hind footpad of C57Bl/6j inbred mice was experimentally infected with 10(7) Leishmania (Leishmania) amazonensis promastigote and the skin was studied through light and electron transmission microscopy and immunohistochemistry (PAP) techniques. RESULTS: There were morphological evidences of cellular immune mechanisms and hypersensitivity reaction after eight weeks of infection and metastasis and well shaped parasites at ultrastructural level by fifty-one weeks post infection. Relapse of infection with mucosa lesions occurred around the 50th week after inoculation. CONCLUSION: The use of this animal model in long term follow up could be an useful experimental model for human mucocutaneous leishmaniasis.

Animals↗
PubMedDate not supplied

[Subcutaneous phaeohyphomycose due to Exophiala jeanselmir. Report of 3 cases in patients with a kidney transplant].

We report three cases of subcutaneous phaeohyphomycosis due to Exophiala jeanselmei (Langeron) McGinnis et Padhye 1977, in kidney transplant patients. Exophiala jeanselmei is a dematious fungus having also ability to rarely procedure eumycetoma (black grains). According to KWON-CHUNG & BENNETT (1992) such fungus is antigenically very heterogeneous, since so far three serotypes have been identified; each serotype including subgroups. Subcutaneous phaeohyphomycosis is becoming more and more frequent in kidney transplant patients submitted to an immunosuppressive treatment. As Exophiala jeanselmei has already been isolated from the environment it becomes difficult to explain the pathogenicity of these cases by a reactivation of quiescent processes. The authors suggest an occasional fungistatic action of cyclosporine A upon Exophiala jeanselmei.

Adult↗