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Biomedical subjects

M N Goldstein

Publications and source records attributed to M N Goldstein.

At least 37 records · Page 2Linked to original sources
PubMed1976

Histochemical demonstration of an increase in acetylcholinesterase in established lines of human and mouse neuroblastomas by nerve growth factor.

Cells of three established lines of human neuroblastoma and an established line of C1300 mouse neuroblastoma were grown in control medium or in experimental medium containing mouse nerve growth factor (NGF). Cultures were stained histochemically for acetylcholinesterase (AChE) during log growth and at confluency. Human neuroblastoma cells grown in medium containing NGF were morphologically more differentiated and they were stained much more intensely for AChE during both phases of growth than were cells in control cultures. The enzyme was distributed over cell bodies and neurites. Neuroblastoma cells of the mouse line were not stimulated to form neurites by NGF, but they were more intensely stained for acetylcholinesterase than cells grown in control medium. These observations support earlier findings that NGF stimulates differentiation of human and mouse neuroblastoma cells in vitro.

Acetylcholinesterase↗
PubMed1975

The long-term effects of callosal sectioning. Report of a second case.

Testing a patient 32 years after callosal sectioning revealed defects in transfer of learning and crossed (mixed modality) matching. This points out that defects may persist long after such surgery and that early reports of negative results of callosal section in patients were probably not correct.

Adolescent↗
PubMed1975

Hereditary myokymia and periodic ataxia.

A kindred in which at least 11 individuals in 3 consecutive generations have continuous muscle movement, i.e., myokymia, and periodic ataxia, has been studied. Three patients, a 24-year-old woman, her 4-year-old son and her 27-year-old sister, have been studied in detail. The disorder is inherited as an autosomal-dominant trait and presents in early childhood with attacks of ataxia of 1-2 min in duration, with associated jerking movements of the head, arms and legs. Attacks are provoked by abrupt postural change, emotional stimulus, and caloric-vestibular stimulation. At the age of 12 years approximately, facial and extremity myokymia appears. Physical findings include large calves, normal muscle strength and widespread myokymia of face, hands, arms and legs with a hand posture resembling carpopedal spasm. EMG studies at rest showed continuous spontaneous activity of otherwise normal motor units. Nerve conduction velocities were normal. Gastrocnemius biopsy in 2 patients showed fiber type grouping and small angular fibers, and was consistent with denervation. Histographic analysis of the biopsies demonstrated enlargement of both fiber types, particularly of Type I fibers. These findings are consistent with chronic denervation and an abnormality of motor neuron population or firing. The myokymia described here is of interest not only because of its genetic association with a movement disorder, but also because the muscle findings support a peripheral basis for the muscle movements.

Adolescent↗
PubMed1975

Amphotericin B-induced sensitivity to actinomycin D in drug-resistant Hela cells.

HeLa cells, which were selected for resistance to actinomycin D on the basis of decreaded penetration of the antibiotic into the cells, were treated with nontoxic concentrations of the polyene antibiotic amphotericin B. In the presence of amphotericin B, the cells became sensitive to the effects of actinomycin D, as demonstrated by loss of cell viability, typical morphological changes, and effects on the pattern of RNA synthesis. Furthermore, we were able to demonstrate that amphotericin B increased the amount of (3H)actinomycin D incorporated into HeLa cells as determined by both radioactive counts and radioautography. Amphotericin B might be useful in overcoming the resistance is based on decreased entry of the agents into cells.

Amphotericin B↗
PubMed1975

Carpal tunnel syndrome in temporal arteritis.

An 88 year old man presented with pain in the hands and wrists, associated with electrodiagnostic studies compatible with a carpal tunnel syndrome in the right wrist. Approximately seven months later he developed manifestations of bilateral temporal arteritis resulting in monocular blindness. Electrodiagnostic studies at this time confirmed the presence of a more severe carpal tunnel syndrome, now present bilaterally. We have been unable to find other case reports of carpal tunnel syndrome related to temporal arteritis. This association may be recognized more frequently if electrodiagnostic studies are performed in patients with temporal arteritis associated with pain in the hands and wrists.

Aged↗
PubMed1974

Effect of nerve growth factor on the expression of colchicine-binding activity and 14-3-2 protein in an established line of human neuroblastoma.

Purified nerve growth factor induced the outgrowth of neurites from cultured human neuroblastoma cells (NJB line) and a concomitant increase in colchicine-binding activity in extracts from these cultures. The parallel stimulation of neurite outgrowth from the cells and colchicine-binding activity of the extracts is interpreted to represent de novo synthesis of microtubular subunit protein in response to the challenge by nerve growth factor. The regulation of the expression of 14-3-2 protein, a protein characteristic of differentiated neuronal cells, was not affected in NJB cells by the addition of nerve growth factor to the culture medium. 14-3-2 protein is present in NJB cells at a concentration equal to that present in human brain from subculture to the stationary phase of growth of the tumor cells. It was concluded that these two gene products, characteristic of differentiated neural cells, are not coordinately regulated in NJB human neuroblastoma cells.

Cell Division↗