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Biomedical subjects

M Musiani

Publications and source records attributed to M Musiani.

At least 109 records · Page 6Linked to original sources
PubMed1986

Double immunoenzymatic staining for the simultaneous detection of Epstein-Barr virus induced antigens.

A double indirect immunoenzymatic staining was developed for the simultaneous visualization of Epstein-Barr virus-induced early antigens and virus capsid antigens in P3HR1 lymphoblastoid cell line. The double immunocytochemical staining was performed with a four-stage and a two-stage procedure employing human sera and monoclonal antibodies against Epstein-Barr virus-induced antigens, followed by the addition of specific alkaline phosphatase and peroxidase labeled antisera. The selection of substrates yielding reaction products of contrasting colours enabled the observer to distinguish cells expressing Epstein-Barr virus capsid antigens (blue) from cells expressing Epstein-Barr virus early antigens (brown).

Alkaline Phosphatase↗
PubMed1986

Stimulating effect of heat shock on the early stage of human cytomegalovirus replication cycle.

The effect of mild heat shock on the replication of human cytomegalovirus (HCMV) was studied in human embryo fibroblasts. Treatment of cell cultures at 44 degrees C for 10 min just before infection or at 24 h post infection (p.i.) shortened HCMV eclipse period and enhanced viral replication, while heat shock performed at 48 h p.i. had no effect on the HCMV replication cycle. Study of HCMV-induced early and late antigens confirmed that the cellular response to heat shock influences HCMV replication in the early stage of the viral replication cycle.

Antigens, Viral↗
PubMed1986

Indirect alkaline phosphatase immunoenzymatic staining for the detection of antibodies to Epstein-Barr virus-induced virus capsid antigens and early antigens.

An indirect alkaline phosphatase immunoenzymatic staining technique was developed for the detection of antibodies against Epstein-Barr virus-induced virus capsid antigens and early antigens in cell smears. The presence of antibodies against Epstein-Barr virus-induced virus capsid antigens and early antigens was revealed by a dark blue staining of cells expressing the antigens. The alkaline phosphatase assay gave a permanent record of the reaction that could be visualized under an ordinary light microscope. The titers obtained with this assay on 91 serum samples were significantly correlated with the titers obtained with an immunofluorescence technique.

Alkaline Phosphatase↗
PubMed1986

Detection of specific immunoglobulin M antibodies to cytomegalovirus by using monoclonal antibody to immunoglobulin M in an indirect immunofluorescence assay.

The detection of immunoglobulin M (IgM) antibodies to cytomegalovirus-induced late antigens by an indirect immunofluorescence assay was improved by the use of monoclonal antibodies to human IgM. Nonspecific background fluorescence was absent, facilitating the reading of the slides and the detection of a specific fluorescence reaction in sera with low levels of specific IgM. Moreover, the indirect immunofluorescence assay with monoclonal antibodies to IgM proved more sensitive than the indirect immunofluorescence assay with polyclonal antibodies to IgM. The absorption of human sera on staphylococcal protein A avoided false reactions due to the presence of rheumatoid factor and high levels of specific IgG in test sera.

Antibodies, Monoclonal↗
PubMed1985

Detection of active Epstein-Barr infection in pregnant women.

Sera taken from pregnant patients and their newborns at delivery were examined for evidence of primary or reactivated Epstein-Barr virus infection. Of 102 women, 37 showed serological signs of reactivated and two signs of primary infection. A mild congenital defect was observed in association with one of the two cases of maternal primary infection. Infants of mothers with reactivated infections remained healthy during the one year follow-up after birth.

Adolescent↗
PubMed1985

Effect of heat shock on Epstein-Barr virus and cytomegalovirus expression.

The effect of heat shock was investigated on lymphoblastoid cell lines Raji and P3HR1 harbouring Epstein-Barr virus (EBV) genomes, and on Vero cells abortively infected with human cytomegalovirus (HCMV). A heat shock at 44 degrees C for 10 min induced the appearance of EBV early antigens in Raji cells and increased the percentage of cells expressing EBV viral capsid antigens in P3HR1 cells. Heat shock performed on Vero cells just before HCMV infection resulted in an approximately fourfold increase in the number of cells exhibiting early nuclear antigens, and in the appearance of HCMV-induced Fc receptors.

Antigens, Viral↗
PubMed1985

Rapid neutralization assay for human cytomegalovirus antibody.

Human cytomegalovirus induces the appearance of immediate early antigens in infected cells 1 h after infection. This provided the basis for the development of a rapid neutralization assay for cytomegalovirus antibody which was able to yield results within a single day. Indirect immunofluorescence to visualize immediate early antigen-positive cells was applied to the rapid determination of cytomegalovirus-neutralizing antibody. The neutralization titers obtained with this assay on 92 serum samples were in accordance with the immune status as determined by enzyme-linked immunosorbent assay to cytomegalovirus-induced immediate early, early, and late antigens.

Antibodies, Viral↗
PubMed1985

Alkaline phosphatase immunoenzymatic staining for detection of antigens induced by cytomegalovirus.

An indirect alkaline phosphatase immunoenzymatic staining method was developed to localise antigens in human embryo fibroblasts that have been induced by cytomegalovirus. The enzyme label was developed with a naphthol salt and fast blue to obtain a bright blue staining of the antigens that could be clearly visualised under an ordinary light microscope. The procedure is rapid, sensitive, and specific and can be used in diagnostic laboratories to detect active infection caused by cytomegalovirus.

Alkaline Phosphatase↗
PubMed1984

Serological screening for the prevention of transfusion-acquired cytomegalovirus infection.

The presence of antibodies against cytomegalovirus (CMV)-induced immediate-early antigens (IEA), early antigens (EA) and late antigens (LA) was sought in 500 healthy blood donors. Antibodies to late antigens were detected in 76% and antibodies to immediate-early and early antigens were detected in 9.6% and 10.2%, respectively. The incidence of antibodies to CMV-induced immediate-early and early antigens increased with age, was higher in females than in males and was related to high values for antibodies against CMV late antigens. Since antibodies to CMV immediate-early and early antigens are significantly associated with active CMV infection, criteria for choosing blood samples unlikely to transmit infective CMV are evaluated.

Adult↗
PubMed1984

Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay.

An enzyme linked immunosorbent assay (ELISA) for detecting antibodies against cytomegalovirus induced immediate early antigens and early antigens was developed using purified nuclear antigens and was compared with the indirect immunofluorescence test. The tests were comparable in their ability to detect positive and negative sera, and antibody titres determined by both assays were similar. The use of ELISA for the detection of antibodies against cytomegalovirus induced immediate early and early antigens is advocated in diagnostic and research laboratories.

Antibodies, Viral↗
PubMed1982

Serum antibody profile against herpesviruses in renal transplant recipients.

Forty-eight renal allograft recipients who had received a kidney graft 2 months to 8 years previously, were followed for the presence of serum antibody against Cytomegalovirus (CMV), Epstein-Barr virus (EBV), Herpes simplex virus (HSV), and Varicella-Zoster virus (VZV). These investigations were performed over a 6 months period and in at least two serum samples from each patient, obtained at intervals of 2-3 months. The presence of serological signs of recent or active infection were observed in 31 patients for CMV, in 22 patients for EBV (18 patients were positive both for CMV and EBV) and only in 3 patients for HSV, while no patients showed serological signs of recent or active infection by VZV.

Adolescent↗
PubMed1979

Inhibition of a complete replication cycle of human cytomegalovirus in actinomycin pre-treated cells.

The study of human cytomegalovirus (HCMV) in cultures of human embryo lung fibroblasts, pre-treated with actinomycin D, has shown that under these conditions the virus infection does not proceed beyond the 'early' events of the virus replication cycle. In the same experimental conditions the growth of poliovirus type I, vaccinia virus and herpes simplex type I virus, was completely unaffected. These results suggest that the complete HCMV replication cycle requires some cellular function(s) between early transcription of the input virus genome and virus DNA synthesis.

Antigens, Viral↗