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Biomedical subjects

M Moya

Publications and source records attributed to M Moya.

At least 55 records · Page 3Linked to original sources
PubMed1995

Studies of the specificities of IgE antibodies found in sera from subjects with allergic reactions to penicillins.

Penicillins are immunogenic when administered to humans and in some instances they can also be allergenic, inducing specific IgE antibodies. Whilst the major haptenic group, the penicilloyl, is well characterised, less is known about the relative importance of the different parts of the structure for antibody binding and how this can influence the specificity of patients response. In order to investigate this further, sera from subjects who had suffered an IgE-mediated reaction to penicillins were studied using the radioallergosorbent test (RAST) and RAST inhibition. The assays employed reagents related to the penicillins causing the reaction. Using 173 sera, positive RAST results were only found with reagents based on benzyl penicillin (BP) and amoxicillin (AX). Fifty-three positive sera were selected for further studies and categorized into three groups: (A) sera only RAST positive to AX, (B) sera only positive to BP and (C) sera positive to both penicillins. RAST inhibition studies were then carried out using monomeric penicilloyl conjugates and compounds representing parts of the penicilloyl structures of BP and AX. For all three groups, monomeric penicilloyl conjugates were the most efficient inhibitors but there were differences for the other compounds. Group A sera were also inhibited by the side chain amoxicillin, whereas group B sera were poorly inhibited by all other inhibitors. Group C sera showed two patterns of inhibition, both consistent with their more cross-reactive profile.

Amoxicillin↗
PubMed1995

[Evaluation of the nutritional status of a rural child population in a Valencia community: the Pego study].

OBJECTIVE: To evaluate the nutritional state of the child population of Pego (Alicante) in order to define the patterns of normality and check the validity of the anthropometric parameters most commonly used in evaluating nutritional status. DESIGN: A descriptive crossover study. SETTING: Valls de Pego Primary Care Health Centre. PATIENTS: 943 healthy children and adolescents aged 0 to 17 were studied out of a target population of 2,150 who were seen for treatment at the Health Centre for reasons which had nothing to do with nutritional problems. INTERVENTIONS: Between july 1989 and december 1990, a simple anthropometric assessment of each patient was carried out: obtaining weight, height, the nutrition or Shukla index and the Quetelet index. MAIN RESULTS: Nutritional distribution was clearly displaced towards high values on the nutritional scale, with 14% obesity and 15% overweight as against 13% at risk of malnutrition. There was more overweight and obesity at a higher age and in males. CONCLUSIONS: This prevalence of nutritional disturbances is similar to that in other Spanish studies and confirms both the size of the problem of obesity in the prevention of related pathologies in adults and the relevance of the adult's Nutritional History throughout his/her life. The study made clear the good correlation and utility of the Shukla and Quetelet indexes.

Adolescent↗
PubMed1993

DSS4-1 is a dominant suppressor of sec4-8 that encodes a nucleotide exchange protein that aids Sec4p function.

The protein Sec4p plays an essential role at the final stage of the yeast secretory pathway and belongs to the ras superfamily of GTP-binding proteins, more specifically to a branch that includes Ypt1p in Saccharomyces cerevisiae and rab proteins in mammalian cells. GTP-binding proteins change conformation depending on whether GTP or GDP is bound and can thus act as a regulatory switch. The protein remains in its inactive, GDP-bound form until exchange of GTP for GDP allows it to stimulate a downstream effector. This interaction is curtailed by GTP hydrolysis. The rates of nucleotide exchange and GTP hydrolysis can be regulated by interaction with accessory proteins. Although GDP dissociation stimulators (GDS) have been identified that act on members of the ras and rho branches of the superfamily, less is known regarding GDSs that act on members of the Sec4/Ypt1/Rab subgroup. A preliminary characterization of a Rab3A GDP dissociation stimulating activity has been presented. We report here the use of suppressor analysis to clone a gene, dss4, encoding a 17K protein that aids Sec4p action in vivo by functioning as a GDP dissociation stimulator.

Amino Acid Sequence↗
PubMed1993

The cycle of SEC4 function in vesicular transport.

Sec4 is a Ras-like GTP-binding protein required for exocytosis in yeast. Unlike Ras, it is the ability of Sec4 to cycle between the GTP- and GDP-bound forms rather than the absolute levels of the GTP-bound form that is critical for function. This cycle may be coupled to an observed cycle of Sec4 localization within the cell. Sec4 binds to secretory vesicles which then fuse with the plasma membrane in exocytosis. Sec4 can recycle from the plasma membrane through a soluble pool to rebind to a new round of vesicles. We have found an activity in yeast (Saccharomyces cerevisiae) comparable to that of the GDP dissociation inhibitor protein isolated from mammalian cells that releases GDP-bound Sec4 from membranes. DSS4-1, a dominant suppressor of the sec4-8 temperature-sensitive mutation, encodes a nucleotide exchange protein. The cycle of Sec4 may function to allow the assembly and subsequent disassembly of a set of proteins necessary for exocytosis. Candidates for members of this set of proteins are encoded by sec genes which show strong genetic interactions with sec4-8. Two of these (SEC8 and SEC15) encode large proteins which form a complex that is peripherally associated with the plasma membrane.

Biological Transport↗
PubMed1992

Short-term polycose substitution for lactose reduces calcium absorption in healthy term babies.

A vitamin D-independent paracellular and not saturable mechanism important for intestinal calcium absorption turned out to be less effective in lactose-free diets. Twelve normal term babies with identical repletion levels of 25-hydroxyvitamin D (25D) were fed sequentially with starting formulas that differed only in their carbohydrate sources: lactose (control) and polycose. A 3-day test was performed after a 1-week adjustment to the new diet. Fecal excretion of calcium was significantly higher in the lactose-free formula period compared to the control one: 56.6 (13.9) versus 39.6 (12.8) mg/kg/day (p less than 0.02); subsequently, the net retention was considerably higher and statistically significant (p less than 0.01) for the control period versus the lactose-free formula period: 99.4 (15.3) versus 78.1 (14.3). Plasma 25D values were 21.8 (9.1) and 23.7 (7.1) ng/ml at the beginning of the lactose and polycose periods, respectively, and 1,25-dihydroxyvitamin-D values were 64 (62) and 75 (45) pg/ml, not significant. Results suggest that formulas containing polycose as the sole carbohydrate source may reduce the intestinal absorption of calcium in term newborns, but determining long-term biological significance requires further observation.

Calcium↗
PubMed1991

Interstitial 15q deletion without a classic Prader-Willi phenotype.

We report on a newborn boy with pronounced hypotonia, cryptorchidism, minor facial anomalies, congenital heart defect, neurologic anomaly, deafness, renal anomaly, and bifid uvula. The patient has a de novo proximal interstitial deletion of chromosome 15 reaching to band q14, larger than that usually seen in Prader-Willi and Angelman syndromes.

Chromosome Banding↗
PubMed1991

Febrile convulsions induced by microwaves and the alteration in behavior of albino mouse OF1.

We studied the effects of microwave (2,440 MHz) induced febrile convulsions (FCs) on the behavior and development of 31 albino mice (OF1 strain), between 25 and 30 days old, in comparison with a group of 33 animals from the same litters. Previously a string test was adapted to measure the neurodevelopment of mice in the first 6 months of life with 82 animals of the same characteristics. The animals submitted to convulsions (fit temperature = 39.2 +/- 0.96 degrees C) showed normal growth, in fact they obtained better results in the test than the control mice (2-way ANOVA, p less than 0.01). FCs have no negative influence on the normal behavior of the mouse.

Animals↗
PubMed1989

Pseudohyperaldosteronism due to application of an antihemorrhoid cream.

Three cases of pseudohyperaldosteronism produced by the application of an antihemorrhoid cream containing 9 alpha-fluoroprednisolone are reported. All three presented edema, hypokalemia and metabolic alkalosis. Two of the patients also exhibited slight arterial hypertension. This iatrogenic possibility must be ruled out in cases of hypermineralocorticoidism, thus avoiding unnecessary and costly examinations.

Administration, Topical↗
PubMed1989

[Computer analysis of single channel current record by extracellular patch clamp].

Neher and Sakmann had provided the presence of the channel protein by the direct measurement of single channel current from excitable membrane in 1976. The advantage of this method "patch clamp" is to isolate a small part of membrane and pick up electrical excitability. In order to record single channel current the seal resistance between patch electrode and cell membrane must be at least more than gigaohm. To improve this recording condition enzyme treated cell or culture cell is necessary since fibroblast which attaches to a target cell disturbs gigaseal formation. In this study we use the computer to improve the single channel current recorded by low seal resistance.

Electric Conductivity↗