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Biomedical subjects

M Motocu

Publications and source records attributed to M Motocu.

13 recordsLinked to original sources

ATP-ase activity of mitochondria isolated from needle-biopsy liver samples of diabetic subjects.

ATP-ase activity in mitochondria isolated from liver needle-biopsy samples was measured in 5 diabetics, aged 30-63 years and in 4 control subjects of similar age. The mitochondrial fraction was isolated by differential centrifugation of the homogenate and the ATP-ase activity was determined in the optimal conditions previously described for human liver mitochondria. The basal and Mg-stimulated ATP-ase activities were higher, while the DNP-stimulated ATP-ase activity was lower in diabetics compared to controls. The ratio of DNP-ATP-ase/Mg2+-ATP-ase was between 1-2 in diabetics and above 5 in controls. This pattern of ATP-ase activity in diabetics is indicative of mitochondrial damage. No quantitative changes in the amount of mitochondria isolated from liver (expressed in micrograms mitochondrial protein/mg wet tissue) could be noticed in diabetics compared to controls. Consequently, the alterations of ATP-ase activity is probably reflective of impairments of functional integrity of liver mitochondria in diabetics.

Adenosine Triphosphatases

Low insulin secretion in decompensated liver cirrhosis with diabetes mellitus.

The present study aimed to investigate the pathogenesis of altered glucose tolerance frequently observed in liver cirrhosis by means of oral glucose tolerance test (OGTT) and i.v. glucagon followed by determining the immunoreactive insulinemia and insulinogenic index (Seltzer). In these conditions of beta islet cells upper limit stimulation a low insulin secretion was found in decompensated liver cirrhosis with alteration of the glucose tolerance (DM or IGT) and a tendency toward hepatic resistance to i.v. glucagon.

Blood Glucose