Biomedical subjects

M Morohashi

Publications and source records attributed to M Morohashi.

115 records · Page 7Linked to original sources

PubMedDate not supplied

Distribution and antimicrobial susceptibility of coagulase-negative staphylococci from skin lesions.

The distribution and antimicrobial susceptibility of coagulase-negative staphylococci from skin lesions were investigated. Staphylococcus epidermidis was found on all areas of the body, whereas S. capitis, S. haemolyticus and S. hominis were mainly found on the face/head or arm/leg. The distribution of coagulase-negative staphylococci in skin lesions and at the same location on normal skin was similar. Staphylococcus lugdunensis was the most susceptible to the nine tested antimicrobials (benzylpenicillin, ampicillin, piperacillin, cefazolin, erythromycin, minocycline, gentamicin, vancomycin and ofloxacin) and S. epidermidis the least susceptible. S. haemolyticus also showed low susceptibility to all nine antimicrobials. Low susceptibility to penicillins may be explained by beta-lactamase production. The existence of coagulase-negative staphylococci, especially concerning their potential pathogenicity and multiple drug resistance, should not be neglected.

Adolescent↗

PubMedDate not supplied

Characterization of Peptostreptococcus species in skin infections.

We examined the characteristics of Peptostreptococcus species in infectious skin diseases. P. magnus was the species identified most frequently, followed by P. asaccharolyticus. Peptostreptococcus species were mainly isolated from infected atheroma and secondary infections due to ulcers; their resistance to five antimicrobial agents was generally low. The resistance of the three predominant Peptostreptococcus species to the antimicrobials was similar to that of all of the Peptostreptococcus species. The predominant Peptostreptococcus species isolated from infected atheroma might be pathogenic. It was considered that the presence of anaerobes as well as aerobes was important to induce the infectious condition.

Adolescent↗

PubMedDate not supplied

Predominant Staphylococcus aureus isolated from various skin diseases.

We examined predominant Staphylococcus aureus isolated from lesions of various skin diseases, and during the past 3 years, 113 methicillin-sensitive and 31 methicillin-resistant S. aureus have been isolated. The predominant species isolated from almost all of the primary bacteriological cultures was S. aureus. The skin diseases from which cultures were most frequently prepared were atopic dermatitis, followed by ulcers. S. aureus was the predominant species in two-thirds or more of cases of all of the different skin diseases examined. The predominant species identified, other than S. aureus, included Streptococcus species and Pseudomonas aeruginosa. Evaluation of the predominance of S. aureus is important to the determination of the severity of skin lesions and, if needed, appropriate antimicrobial therapy.

Dermatitis, Atopic↗

PubMedDate not supplied

An ultrastructural and immunohistochemical study of pigmented dermatofibrosarcoma protuberans (Bednar tumor).

A case of Bednar tumor on the right shoulder of a 47-year-old Japanese woman is reported. Histological examination showed plump, spindle cells arranged in a storiform pattern in central areas of the tumor and a diffuse infiltration of the dermal stroma, which was frequently extended into the subcutis at the periphery of the tumor. The tumor contained a fairly identified population of dendritic pigmented cells. Ultrastructurally, most cells had folded nuclei, were spindle-shaped and had long, slender cytoplasmic projections. Dendritic pigmented cells, which were dispersed among neoplastic cells, contained premelanosomes and mature melanosomes. Immunohistochemically, tumor cells exhibited positive reactions for vimentin and CD 34 and failed to show a positive reaction for neuron specific enolase, HMB-45 or S-100 protein. Factor X IIIa was only expressed on tumor cells around melanin-containing cells, which reacted positively with antibodies to S-100 protein and vimentin. These results indicate that the phenotype of tumor cells around melanin-containing cells differs from other tumor cells and that this difference may be caused by the relationship of tumor cells and melanin-containing cells.

Dendritic Cells↗

PubMedDate not supplied

Infundibulocystic basal cell carcinoma.

We report on a 72-year-old male patient with a solitary, dome-shaped tumor on the preauricular region. Histologically, it showed an intradermal basaloid cell tumor composed of many anastomosing epithelial cords. There were multiple infundibular cystic structures containing corneocytes lined by follicular infundibular epithelium and bud-like structures simulating the follicular germs throughout the neoplasm. Follicular bulbs and papillae were absent within the tumor. The stroma was scant and not highly fibroplastic. The tumor was diagnosed as infundibulocystic basal cell carcinoma, a recently described, uncommon variant of basal cell carcinoma.

Aged↗

PubMedDate not supplied

Dyskeratosis congenita: a light microscopic and ultrastructural study.

We have examined, histologically and ultrastructurally, a case of dyskerato-sis congenita in a 46-year-old man. Clinically, a net-like pigmentation with partial poikiloderma atrophicans vasculare was observed. Dystrophic changes of the nails and whitish thickening of the oral mucosa were also present. Histological examination showed atrophy of the epidermis, disappearance of the rete ridges and cleft formation of dermo-epidermal junction. Vacuoles could be seen on electron microscopy in the cytoplasm of basal cells and above the basal lamina. Tonofibrils in the cytoplasm of basal cells were decreased in number and size. Duplication or multiplication of basal lamina was also seen. From these results, it appears that the vacuoles observed ultrastructurally correspond to the cleft of the dermo-epidermal junction seen by light microscopy and that atrophy of the epidermis and/or disappearance of rete ridges result from sporadic degeneration of basal cells.

Biopsy↗

PubMedDate not supplied

A specific cutaneous lesion revealing myelodysplastic syndrome.

We report on an 86-year-old man with an ulcerated nodule on his left lower leg. Peripheral blood examination and bone marrow findings were compatible with the refractory anemia with an excess of blasts in transformation (RAEB-T) which is typical of the myelodysplastic syndrome (MDS). Because histological examination showed an infiltration of atypical cells of myeloid origin, this lesion was diagnosed as a specific lesion of MDS. Sometimes, only a subjective symptom, such as a skin lesion, precedes the diagnosis of MDS.

Aged↗