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Biomedical subjects

M Moore

Publications and source records attributed to M Moore.

At least 523 records · Page 29Linked to original sources
PubMed1973

Immunogenicity of rat hepatoma membrane fractions.

The principal expression of immunity elicited in syngeneic rats immunized with rat hepatoma membrane fractions was the development of a tumour specific antibody response. This antibody was demonstrable by membrane immunofluorescence staining of viable hepatoma cells in suspension and the sera exhibited complement dependent cytotoxicity for cultured hepatoma cells. In the absence of complement, however, membrane immune sera were highly "blocking", protecting plated hepatoma cells from attack by sensitized lymph node cells. The cell mediated immune response elicited by hepatoma membrane immunization was weak, as evaluated by the colony inhibitory activity of lymph node cells for hepatoma cells in vitro or the adoptive transfer of immunity with peritoneal exudate cells. Correlated with this overall pattern of immune response, membrane immunization did not elicit tumour rejection reactions. These findings are relevant to current views that humoral factors operate antagonistically to limit cell mediated immunity to tumours. A further relevant feature was the observation that membrane immunization, eliciting a prominent humoral immune reaction, conditioned the recipients so that they subsequently failed to elicit a tumour rejection immunity on treatment with irradiated tumour cells.

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PubMed1973

Tumour-associated transplantation antigens of neoplasms induced by a naturally occurring murine sarcoma virus (FBJ-MSV).

FBJ osteosarcoma virus (FBJ-MSV) isolated originally from a spontaneously arising osteosarcoma in a CF1 mouse is the only known naturally occurring murine sarcoma virus (MSV). It is unique among strains of MSV in producing primarily sarcomata in mice. The capacity of tumour cells transformed in vivo by this agent to elicit specific transplantation immunity in syngeneic hosts was investigated. A low level of resistance (10(4)-10(5) cells) was consistently induced by implantation of x-irradiated (15,000 rad) tumours or surgical excision of developing subcutaneous grafts. By contrast intraperitoneal inoculation of virus containing cellfree extracts of FBJ-MSV sarcomata was a far less effective immunization procedure. Confirmatory evidence for the antigenicity of these neoplasms was obtained in tests in which preincubation of tumour cells with lymphoid cells from specifically immune donors inhibited in vivo outgrowth of the FBJ-MSV cells in untreated syngeneic recipients. The induction of host resistance to FBJ-MSV cells by immunization with identical and independently-induced FBJ-MSV tumours established that FBJ-MSV cells possess common cell surface antigenic specificities in a manner analogous to those of experimental neoplasms induced by other oncogenic DNA and RNA viruses. Since FBJ-MSV cells release infectious virus it was not possible in this system to establish whether the tumour-rejection antigen was cellular or virion in nature. The antigenic weakness of FBJ-MSV cells in syngeneic hosts is comparable with that of virus-induced murine leukaemias of the Gross (G) or "wild" type subgroup to which category FBJ-MSV also belongs. These features suggest that FBJ-MSV exemplifies naturally occurring sarcomagenic viruses more closely than those of the Friend-Moloney-Rauscher-Graffi (FMRGr) subgroup which in general induce highly antigenic neoplasms.

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PubMed1972

Studies on the antigenicity of radiation-induced murine osteosarcomata.

The immunogenicities of 15 murine osteosarcomata induced with a bone seeking radioisotope ((90)Sr) in normal and chimaeric CBA mice were studied. Attempts were made to induce tumour-specific immunity in syngeneic mice by treatment with x-irradiated (15,000 rad) tumour or surgical excision of developing subcutaneous tumour grafts. Resistance was evoked against 6 tumours and this was relatively weak. With the remaining tumours, no resistance against the immunizing tumour could be demonstrated, even though the transplantation tests were made highly sensitive by the use of inocula of as few as 2 × 10(3) cells in pre-irradiated (400 rad) hosts. Sera from mice immunized against each of the tumours were tested against viable cells of the immunizing tumour by indirect immunofluorescence. In no instance did tumour antisera give a convincing reaction with tumour cells although alloantisera raised by hyperimmunization of H-2 identical and H-2 different donors with osteosarcomata consistently gave strongly positive reactions. The results are interpreted as illustrating the weak tumour specific antigenicity of radiation-induced murine osteosarcomata. The possibility that antigenic deficiency is a consequence of immunosurveillance in this tumour system is discussed.

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PubMed1972

FBJ virus-induced tumours in mice. A histopathological study of FBJ virus tumours and their relevance to murine and human osteosarcoma arising in bone.

Nine of the 15 neonatal CBA mice injected intramuscularly with a Moloney concentrate containing FBJ virus developed tumours: likewise 5 of 7 CBA neonates injected intraperitoneally with a cell-free filtrate derived from a transplanted tumour of the former group.Of soft tissue origin, these FBJ sarcomata have a characteristic histological appearance and are of low grade malignancy. Although occasional islets of cartilage osteoid and bone were noted, these were regarded as indicative of evolutionary metaplasia in the collagenous matrix of pleomorphic fibroblastic sarcoma. No tumour was acceptable as osteosarcoma of conventional type and osseous origin. There were, however, additionally 2 minute spindle cell sarcomata arising in femoral periosteum and non-neoplastic periosteal proliferation was observed. The differences of these FBJ fibroblastic sarcomata from murine osteosarcoma-either spontaneous or induced by Sr 90-are emphasized. Furthermore, their deviation from the structural pattern and behaviour of human osteosarcoma is discussed.

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