Search PubMed⌕ Search

Biomedical subjects

M Moon

Publications and source records attributed to M Moon.

At least 19 recordsLinked to original sources
PubMed2005

DEG1, encoding the tRNA:pseudouridine synthase Pus3p, impacts HOT1-stimulated recombination in Saccharomyces cerevisiae.

In Saccharomyces cerevisiae, HOT1-stimulated recombination has been implicated in maintaining homology between repeated ribosomal RNA genes. The ability of HOT1 to stimulate genetic exchange requires RNA polymerase I transcription across the recombining sequences. The trans-acting nuclear mutation hrm3-1 specifically reduces HOT1-dependent recombination and prevents cell growth at 37 degrees . The HRM3 gene is identical to DEG1. Excisive, but not gene replacement, recombination is reduced in HOT1-adjacent sequences in deg1Delta mutants. Excisive recombination within the genomic rDNA repeats is also decreased. The hypo-recombination and temperature-sensitive phenotypes of deg1Delta mutants are recessive. Deletion of DEG1 did not affect the rate of transcription from HOT1 or rDNA suggesting that while transcription is necessary it is not sufficient for HOT1 activity. Pseudouridine synthase 3 (Pus3p), the DEG1 gene product, modifies the anticodon arm of transfer RNA at positions 38 and 39 by catalyzing the conversion of uridine to pseudouridine. Cells deficient in pseudouridine synthases encoded by PUS1, PUS2 or PUS4 displayed no recombination defects, indicating that Pus3p plays a specific role in HOT1 activity. Pus3p is unique in its ability to modulate frameshifting and readthrough events during translation, and this aspect of its activity may be responsible for HOT1 recombination phenotypes observed in deg1 mutants.

Alleles↗
PubMed2001

Medicare.

Explore the source record for details and available documents.

Aged↗
PubMed2001

Pharmacokinetics of sufentanil in patients undergoing coronary artery bypass graft surgery.

OBJECTIVE: To determine the pharmacokinetics of sufentanil in patients undergoing coronary artery bypass graft surgery. DESIGN: Prospective, multigroup study. SETTING: University-affiliated hospital. PARTICIPANTS: Patients with good left ventricular function undergoing elective surgery (n = 103). INTERVENTIONS: Sufentanil was administered by target-controlled infusion, with target effect-site concentrations ranging from 0.4 to 4.5 ng/mL. Isoflurane was administered as required to maintain stable hemodynamics. Sufentanil pharmacokinetics were determined by population modeling. The potential effects of gender, weight, different premedications (lorazepam, morphine-scopolamine, or clonidine), and coinduction with propofol on sufentanil pharmacokinetics were explored. MEASUREMENTS AND MAIN RESULTS: The first model determined was a simple 3-compartment model, without any covariates, which had these parameters: V(1) = 5.7 L, V(2) = 18.1 L, V(3) = 225 L, Cl(1) = 0.69 L/min, Cl(2) = 3.1 L/min, and Cl(3) = 1.4 L/min. The overall predictive ability during the entire pre-cardiopulmonary bypass period of this model was excellent, with virtually no bias (median prediction error, -0.4%) and good precision (median absolute prediction error, 18.4%). More complex models with the various premedications used or coinduction with propofol as covariates did not improve the predictive accuracy or precision compared with the simple 3-compartment model. Similarly, including either gender or weight as a covariate did not improve predictive ability. CONCLUSION: The authors have determined a pharmacokinetic model for sufentanil that can be used to maintain desired target concentrations of sufentanil before cardiopulmonary bypass, with a high degree of accuracy and acceptable variability. Concomitantly administered medications (lorazepam, morphine-scopolamine, clonidine, or propofol) do not appear to have any clinically important effects on distribution-phase sufentanil pharmacokinetics.

Aged↗
PubMed1999

Does sufentanil concentration influence isoflurane requirements during coronary artery bypass grafting?

OBJECTIVE: To search for concentration-related suppression of hemodynamic responsiveness by sufentanil. DESIGN: Prospective, randomized, double-blind study. SETTING: University hospital. PARTICIPANTS: Patients undergoing elective coronary artery bypass grafting (CABG). INTERVENTION: Patients were assigned to target effect-site sufentanil concentrations of 1.5 ng/mL (group L; n = 14), 3.0 ng/mL (group M; n = 13), or 4.5 ng/mL (group H; n = 12). Sufentanil was administered by computer-assisted continuous infusion. Isoflurane was used to maintain intraoperative hemodynamics near preoperative values. MEASUREMENTS AND MAIN RESULTS: Hemodynamics, the electroencephalographic spectral edge (SE95), and end-tidal isoflurane concentration (ET-ISO) were measured every 10 to 30 seconds during the prebypass period. Serum sufentanil concentration was measured at intervals. Prebypass serum sufentanil concentrations were stable, averaging 3.0 +/- 0.7, 5.1 +/- 1.1, and 7.1 +/- 1.3 ng/mL in groups L, M, and H, respectively. The groups did not differ with respect to the speed of induction, intraoperative hemodynamics, incidence of isoflurane use, or isoflurane concentrations required. ET-ISO and serum sufentanil levels were not correlated. Among seven group L patients who did not require isoflurane, the average prebypass serum sufentanil concentration ranged from 1.7 to 3.3 ng/mL. CONCLUSION: Sufentanil does not induce concentration-related suppression of hemodynamic responsiveness over the range studied. A stable serum sufentanil concentration of 3.0 +/- 0.7 ng/mL induces the maximal opioid effect and need not be exceeded in patients undergoing CABG. A sufentanil concentration of 1.7 ng/mL provides clinically adequate anesthesia without supplementation in some premedicated patients undergoing CABG.

Aged↗
PubMed1999

Women's characteristics and gender role attitudes: support for father involvement with children.

Women's (N = 364) personal characteristics and gender role attitudes were examined in relation to their support for father involvement with children. The respondents completed measures of trust, attitudes toward women, hostility, self-esteem, and father involvement. Nontraditional gender role attitudes, positive ratings of their own interpersonal trust, and low hostility toward men were predictive of the respondents' support for father involvement. Participant demographics (including age, marital status, and number of children) were unrelated to their views of father involvement. Results indicate the importance of considering the characteristics and attitudes women bring to the co-parental relationship in the examination of factors influencing father involvement with children. Findings are discussed within the context of mothers' primary child-care and gatekeeping roles.

Adolescent↗
PubMed1999

Can Medicare survive its saviors?

It is now an open question whether Medicare will provide adequate health coverage to the baby-boom generation as it begins turning 65 just over a decade from now. The source of uncertainty is not whether America has the resources to sustain the program; we do. The real challenge comes instead from proposals to save Medicare. Far from preserving its benefits, the major restructuring proposals under discussion would radically alter the principles on which Medicare rests and erode the protection it affords.

Aged↗
PubMed1999

State-level variation in Medicare spending.

Theoretically, Medicare provides one standard benefit package to all enrollees. But because of State-level variations in populations, service supply, and local practice patterns, national policy changes may have unequal impacts on access and service utilization. Across-the-board policy changes may create hardships in one area while appropriately discouraging use in another area. In this article, the authors describe State-level variations in Medicare enrollees, their insurance coverage, 1995 Medicare and beneficiary spending patterns in aggregate, per capita, and by service, and certain spending patterns for dually eligible beneficiaries. These data are useful for considering the State-level effects of payment reform.

Accounting↗
PubMed1998

The significance of donor leg distal runoff in femorofemoral bypass grafting.

BACKGROUND: The aim of this study is to determine if femorofemoral bypass grafting is an effective operation for unilateral iliac occlusive disease, and to assess for factors that may predict a poorer outcome. A retrospective review was conducted of cases identified from a prospective audit database. METHODS: Information was obtained from the clinical notes of 97 patients who had 100 femorofemoral bypass graft operations. All living patients were invited for assessment which included colour duplex sonography of the graft and measurement of the ankle brachial index. RESULTS: Using life-table analysis, the five-year graft secondary patency for claudicants was 79% and for those with critical ischaemia 78%. Five-year survival for claudicants was 65% and for those with critical ischaemia, 38%. Poor distal runoff in the infrapopliteal arteries of the donor limb adversely affected graft patency. Graft survival was not affected by the indication for operation, by donor or recipient superficial femoral artery patency, or by the need for endarterectomy, pre-operative angioplasty or the graft material. CONCLUSIONS: Femorofemoral bypass grafting is the procedure of choice for all patients who require surgical intervention for unilateral iliac occlusion and donor limb distal runoff may be an important determinant of graft patency.

Adult↗
PubMed1998

Options for aiding low-income Medicare beneficiaries.

This paper examines the effectiveness of the Qualified Medicare Beneficiary (QMB), Specified Low-Income Medicare Beneficiary (SLMB), and Qualified Individual (QI) programs that seek to ease the financial burdens of health costs on low-income Medicare beneficiaries. While participation in these programs has been increasing steadily over time, much remains to be done to extend benefits to all those currently eligible. This paper estimates levels of participation in the existing programs and considers several scenarios under which participation could be increased further, including federalizing the existing programs, raising or eliminating asset limits, and raising income ceilings.

Eligibility Determination↗
PubMed1997

Difference in interaction of fibronectin with type I collagen and type IV collagen.

In our studies on fibronectin, difference in binding to type I collagen and type IV collagen was observed and analysed. Four different fragments, which consist of I6-II1-II2-I7-I8-I9, I6-II1-II2-I7, I6-II1-II2, and I8-I9 within the collagen binding domain, have been isolated from proteolytic digests of fibronectin. The N-terminal fragments of the collagen binding domain showed the binding affinity to both of type I and type IV collagens. On the other hand, the C-terminal portion of the domain, I8-I9, only bound to type I collagen. The newly developed monoclonal antibody FN 40, which recognizes type I9 homology repeat of the collagen binding domain, inhibited the binding of fibronectin to type I collagen in a dose-dependent manner. Three overlapping fragments, I6-II1-II2-I7, I6-II1-II2-I7-I8, and I7-I8-I9, which have been expressed in Escherichia coli, showed the similar binding affinity to type IV collagen, whereas the fragment containing type I9 repeat (I7-I8-I9) showed the significantly stronger binding activity to type I collagen. In addition, the expressed fragment lacking I9 competitively inhibited the binding of fibronectin to type IV collagen. In contrast, the interference of this fragment to type I collagen binding activity of fibronectin was not significant. These data indicate that the collagen binding domain contains at least 2 sites for interaction with each type of collagen and that type I9 repeat is important for type I collagen binding, whereas I7 is for type IV collagen binding. Chemical modification studies revealed that the three-dimensional structure of fibronectin is more important for type I collagen binding.

Antibodies, Monoclonal↗
PubMed1997

Maintenance of aerobic capacity during recovery from right foot Jones fracture: a case report.

PURPOSE: We sought to evaluate the effectiveness of continued training with deep water running (DWR) on the healing process from a lower leg fracture in maintaining aerobic fitness. CASE SUMMARY: An elite male middle distance runner presented with a Jones fracture of the right foot. The patient opted for nonsurgical treatment. Non-weight-bearing immobilization in a cast proceeded for 14 weeks. The patient continued training by simulating land running and team land workouts with DWR. DWR was the principal mode of exercise through week 24. Physiological and race performance assessments noted a progressive and complete return to preinjury fitness and performance levels. DISCUSSION: DWR can be used by competitive runners during a rehabilitative period to maintain fitness and does not impede the healing process. In developing a DWR regimen, it should be noted that the exercise heart rate when working above the ventilatory threshold will be approximately 12 bpm lower in water. RELEVANCE: DWR may be feasible for maintenance of competitive edge during lower trunk injury rehabilitation.

Adult↗